稻纵卷叶螟颗粒体病毒亚致死作用对宿主生长、繁殖和生理生化的影响

doi:10.16409/j.cnki.2095-039x.2022.09.024

摘要/Abstract

摘要： 稻纵卷叶螟颗粒体病毒(CnmeGV)是感染稻纵卷叶螟的专性杆状病毒。明确CnmeGV亚致死作用对稻纵卷叶螟生长发育、繁殖能力和生理生化的影响,为应用CnmeGV防治稻纵卷叶螟提供依据。CnmeGV 1×10⁵ OB/g浓度处理3龄稻纵卷叶螟幼虫10 d后存活幼虫虫体内仍普遍检测到CnmeGV的存在,从而产生亚致死作用。亚致死CnmeGV处理对稻纵卷叶螟的生长发育产生明显影响,主要表现在变态发育的化蛹、羽化和产卵过程,存活个体蛹重减轻了15.95%,羽化率下降了24.81%,产卵量减少41.5%,差异显著。同时雌雄性比从1.11下降到0.82。CnmeGV亚致死处理诱导幼虫能量代谢增强,中肠消化酶中淀粉酶、脂肪酶活性显著提高,而解毒酶中羧酸酯酶活性显著下降,谷胱甘肽转移酶活性显著提高。CnmeGV亚致死作用影响到虫体激素分泌,幼虫体内蜕皮激素、保幼激素水平较对照分别下降了4.59和5.62倍。CnmeGV亚致死处理雌成虫卵黄蛋白含量下降了40.63%,可能是导致产卵量降低的主要因素。

关键词: 稻纵卷叶螟, 颗粒体病毒, 亚致死作用, 生长发育

Abstract: Cnaphalocrocis medinalis granulovirus,CnmeGV,is a specific pathogenic baculovirus of rice pest C.medinalis.This study analyzed the sub-lethal effects of CnemGV on the growth,reproduction,and physiology of C.medinalis to provide a theoretical basis for the application of the baculovirus for C.medinalis control.CnmeGV was generally detected in the surviving larvae at 10 days post treatment of 1×10⁵ OB/g dose of CnmeGV to the 3^rd instar larvae of C.medinalis,resulting in sub-lethal effect on C.medinalis.Sub-lethal effect greatly affected the development of C.medinalis,especially in the metamorphosis process of pupation,eclosion,and oviposition.The pupal weight was decreased by 15.95%,eclosion rate was reduced by 24.81%,oviposition was dropped by 41.5%,significantly different from that of the control.The ratio of female to male was also declined from 1.11 to 0.82.The sub-lethal effect of CnmeGV enhanced the energy metabolism of larvae,with the activities of amylase and lipase raised remarkably.Among detoxification enzymes,the activity of GST was improved,while ACT level was dropped significantly compared to that of the control.The sub-lethal of CnmeGV influenced the secretion of hormones.The levels of juvenile and molting hormone were declined by 5.62 and 4.59 folds,respectively.Vitellogenin content was reduced at about 40.63% in the sub-lethal treatment,which may be responsible for the reduced oviposition rate.

Key words: Cnaphalocrocis medinalis, granulovirus, sub-lethal effect, development and reproduction

中图分类号:

S476.1

刘琴, 李传明, 韩光杰, 徐彬, 黄立鑫, 陆玉荣, 夏杨, 徐健. 稻纵卷叶螟颗粒体病毒亚致死作用对宿主生长、繁殖和生理生化的影响[J]. 中国生物防治学报, 2022, 38(4): 982-989.

LIU Qin, LI Chuanming, HAN Guangjie, XU Bin, HUANG Lixin, LU Yurong, XIA Yang, XU Jian. Sub-lethal Effect of Cnaphalocrocis Medinalis Granulovirus to the Growth,Reproduction and Physiology of Host[J]. Chinese Journal of Biological Control, 2022, 38(4): 982-989.

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