马铃薯疮痂病拮抗菌ZF517的分离鉴定及生防效果初步研究

doi:10.16409/j.cnki.2095-039x.2025.02.020

中国生物防治学报 ›› 2025, Vol. 41 ›› Issue (2): 411-422.DOI: 10.16409/j.cnki.2095-039x.2025.02.020

• 研究论文 • 上一篇

马铃薯疮痂病拮抗菌ZF517的分离鉴定及生防效果初步研究

卫鑫辰^1,2, 刘易³, 杨茹薇³, 段广荣⁴, 谢学文¹, 石延霞¹, 柴阿丽¹, 李宝聚¹, 李磊¹

1. 中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室, 北京100081;
2. 天津农学院园艺园林学院, 天津 300384;
3. 新疆农业科学院综合试验场, 乌鲁木齐 830011;
4. 中化作物保护品有限公司, 上海 200121

收稿日期:2024-11-27 发布日期:2025-04-19
通讯作者: 李磊, 李宝聚
作者简介:卫鑫辰, 男, 硕士研究生, E-mail:weixinchen2022@163.com;刘易, 男, 硕士, 研究员, E-mail:liuyun_5511@163.com;通信作者, 李磊, 男, 博士, 副研究员, E-mail:lilei01@caas.cn;李宝聚, 男, 博士, 研究员, E-mail:libaojuivf@163.com。
基金资助:
新疆维吾尔自治区重点研发计划项目（2022B02044）；中国农业科学院科技创新工程项目（CAAS-ASTIP-IVFCAAS）；农业农村部园艺作物生物学与种质创制重点实验室（IVF2023）；蔬菜生物育种全国重点实验室开放课题

Isolation and Identification of Bacillus subtilis ZF517 and Its Control Effect on Potato Common Scab

WEI Xinchen^1,2, LIU Yi³, YANG Ruwei³, DUAN Guangrong⁴, XIE Xuewen¹, SHI Yanxia¹, CHAI Ali¹, LI Baoju¹, LI lei¹

1. State Key Laboratory of Vegetable Biobreeding/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. College of Horticultural and Landscape Architecture, Tianjin Agricultural University, Tianjin 300384, China;
3. Xinjiang Academy of Agricultural Sciences, Comprehensive Experimental Station, Urumqi 830011, China;
4. Sinochem Crop Protection Co., Ltd., Shanghai 200121, China

Received:2024-11-27 Published:2025-04-19

摘要/Abstract

摘要： 马铃薯疮痂病（potato common scab）近年来在我国主要产区广泛发生，造成严重危害，且缺乏绿色高效的防治措施。为筛选对疮痂病具有显著拮抗效果的生防菌株，本研究以疮痂链霉菌Streptomyces scabies为靶标，筛选到一株拮抗效果显著的生防菌株ZF517。通过形态学观察、生理生化特性测定以及多基因系统发育树分析，鉴定该菌株为枯草芽胞杆菌Bacillus subtilis。抑菌谱分析显示，菌株ZF517对6种病原真菌和3种病原细菌具有较高的拮抗效果。生防性状检测表明，ZF517能产生蛋白酶和嗜铁素，并含有Surfactin、Macrolactin、Fengycin、Difficidin、Bacillibactin、Bacillaene、Bacilysin和Iturin的合成基因。此外，ZF517的发酵液可抑制疮痂链霉菌菌丝的生长。经萝卜幼苗法验证，ZF517发酵液可显著抑制疮痂链霉菌对萝卜幼苗的侵染，其防效达到63.94%；盆栽试验结果表明，其对马铃薯疮痂病的防效达58.07%，显著优于对照药剂20%噻唑锌悬浮剂的防治效果。综上，菌株ZF517在防治马铃薯疮痂病方面表现出较大的生防潜力。

关键词: 枯草芽胞杆菌, 马铃薯疮痂病, 脂肽类物质, 生物防治

Abstract: Potato common scab, a severe soilborne disease caused by Streptomyces scabies, has become increasingly prevalent in major potato-producing regions in China, causing significant yield and quality losses. However, effective and environmentally friendly control strategies remain limited. In this study, a biocontrol strain, ZF517, exhibiting strong antagonistic activity against S. scabies, was isolated. Based on morphological characteristics, physiological and biochemical tests, and multi-gene phylogenetic analysis, ZF517 was identified as Bacillus subtilis. Antagonistic spectrum analysis revealed that ZF517 exhibited potent inhibitory effects against six pathogenic fungi and three pathogenic bacteria. Biocontrol-related traits analysis showed that ZF517 secretes proteases and siderophores and harbours biosynthetic genes for Surfactin, Macrolactin, Fengycin, Difficidin, Bacillibactin, Bacillaene, Bacilysin, and Iturin. Moreover, the fermentation broth of ZF517 significantly inhibited the mycelial growth of S. scabies. A radish seedling assay confirmed that the fermentation broth effectively suppressed S. scabies infection, achieving a biocontrol efficacy of 63.94%. In pot experiments, ZF517 exhibited a biocontrol efficacy of 58.07% against potato common scab, significantly surpassing the efficacy of the chemical control treatment (20% thiazole zinc suspension concentrate). These findings indicate that ZF517 holds great potential as a biocontrol agent for managing potato common scab.

Key words: Bacillus subtilis, potato common scab, lipopeptides, biological control

中图分类号:

S476

卫鑫辰, 刘易, 杨茹薇, 段广荣, 谢学文, 石延霞, 柴阿丽, 李宝聚, 李磊. 马铃薯疮痂病拮抗菌ZF517的分离鉴定及生防效果初步研究[J]. 中国生物防治学报, 2025, 41(2): 411-422.

WEI Xinchen, LIU Yi, YANG Ruwei, DUAN Guangrong, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Baoju, LI lei. Isolation and Identification of Bacillus subtilis ZF517 and Its Control Effect on Potato Common Scab[J]. Chinese Journal of Biological Control, 2025, 41(2): 411-422.

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马铃薯疮痂病拮抗菌ZF517的分离鉴定及生防效果初步研究

Isolation and Identification of Bacillus subtilis ZF517 and Its Control Effect on Potato Common Scab

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