群体感应信号分子3-oxo-C8-HSL对褐飞虱肠道共生菌多样性的影响

doi:10.16409/j.cnki.2095-039x.2024.07.009

摘要/Abstract

摘要： 由酰基高丝氨酸内酯（acyl homoserine lactone，AHL）介导的细菌群体感应（quorum sensing，QS）是革兰氏阴性细菌中广泛存在的调控系统。然而，对于QS感应与昆虫肠道菌群的互作以及QS与肠道免疫的关系尚未明确。本试验通过向褐飞虱Nilaparvata lugens St?l饲喂细菌QS信号分子——三氧代辛酰基高丝氨酸内脂（3-oxo-C8-HSL），探索QS对昆虫肠道微生物群落多样性及肠道免疫的影响。结果表明，3-oxo-C8-HSL处理第5 d的褐飞虱死亡率较对照组高出61.39%，生存时间缩短5 d。褐飞虱肠道微生物菌门、菌属和物种数（ASV）较对照组减少，细菌多样性升高，真菌多样性降低；细菌和真菌群落的原优势菌属相对丰度降低。与此同时，3-oxo-C8-HSL激活肠道免疫，导致免疫效应物大量表达。综上所述，3-oxo-C8-HSL通过改变褐飞虱肠道微生物群落的多样性并激活褐飞虱的肠道免疫响应，实现有效的杀虫效果，该方法为未来寻找到更有效的害虫防治方法提供新策略。

关键词: 褐飞虱, 细菌群体感应, 肠道共生菌, 免疫基因, 微生物多样性

Abstract: Bacterial quorum sensing (QS) mediated by acyl homoserine lactone (AHL) molecules is a widespread regulatory system in Gram-negative bacteria. However, the interactions and intestinal immunity between QS and insect gut microbes have not been clarified. In this experiment, we explored the effects of QS on the diversity of insect gut microbial communities and intestinal immunity by feeding a bacterial community sensing signaling molecule, 3-oxooctanoyl-homoserine lactone (3-oxo-C8-HSL), to the Nilaparvata lugens Stål. The results showed that the mortality of N. lugens on the fifth day after 3-oxo-C8-HSL treatment was 61.39% higher than that of the control group, and the survival time was shortened by 5 days. The number of microbial phyla, genera and species (ASV) in the intestinal microorganisms of N. lugens were reduced compared with that of the control group, and the diversity of bacteria was increased and the diversity of fungi was decreased; the relative abundance of the dominant genera in the bacterial and fungal communities was reduced. At the same time, the treatment activated intestinal immunity and upregulated expression of immune effectors. In summary, 3-oxo-C8-HSL had effective insecticidal activity, altered the diversity of N. lugens gut microbial communities and activated the gut immune response of N. lugens.

Key words: Nilaparvata lugens, bacterial quorum sensing, intestinal symbiotic microbes, immune genes, microbial diversity

中图分类号:

S476.1

范琳琳, 宋阳, 申屠旭萍, 俞晓平. 群体感应信号分子3-oxo-C8-HSL对褐飞虱肠道共生菌多样性的影响[J]. 中国生物防治学报, 2025, 41(3): 710-720.

FAN Linlin, SONG Yang, SHENTU Xuping, YU Xiaoping. Effect of Quorum Sensing Signaling Molecule 3-oxo-C8-HSL on Intestinal Symbiont Microbial Diversity of Nilaparvata lugens Stål[J]. Chinese Journal of Biological Control, 2025, 41(3): 710-720.

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