高通量测序分析高温覆膜对韭菜根际微生物多样性的影响

doi:10.16409/j.cnki.2095-039x.2020.06.024

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (6): 938-945.DOI: 10.16409/j.cnki.2095-039x.2020.06.024

• 研究论文 • 上一篇

高通量测序分析高温覆膜对韭菜根际微生物多样性的影响

宋健¹, 张海剑¹, 刘莉², 杜立新³, 柳健虎¹, 曹伟平¹

1. 河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;
2. 河北省植保植检总站, 石家庄 050000;
3. 廊坊师范学院生命科学学院, 廊坊 065000

收稿日期:2019-12-20 发布日期:2021-01-09
通讯作者: 曹伟平,研究员,E-mail:cwplx751209@163.com。
作者简介:宋健,副研究员,E-mail:sj3250@163.com
基金资助:
国家重点研发计划（2017YFD0201205）；河北省自然科学基金（C2018301023）；河北省重点研发项目（19226510D）；河北省农村科学院现代农业科技创新工程（2019-1-03）；河北省农林科学院科学技术研究与发展计划项目（2018120302）

Effect of High Temperature Film Mulching on Soil Microbial Functional Diversity by High Throughput Sequencing

SONG Jian¹, ZHANG Haijian¹, LIU Li², DU Lixin³, LIU Jianhu¹, CAO Weiping¹

1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;
2. Hebei Provincial General Station of Plant Protection and Quarantine, Shijiazhuang 050000, China;
3. College of Life Sciences, Langfang Normal University, Langfang 065000, China

Received:2019-12-20 Published:2021-01-09

摘要/Abstract

摘要： 为明确高温覆膜对韭菜根际土壤微生物群落多样性和组成的影响。本研究在覆膜前后对韭菜根际土壤中总DNA进行提取，采用Illumina Hiseq 2500高通量测序技术对土壤中细菌的16S rDNA和真菌的ITS基因进行了序列测定，分析了高温覆膜前后韭菜根际土壤中微生物群落结构特征。结果表明，高温覆膜前后分别获得细菌OTUs 1849个和1819个，真菌OTUs 148个和151个，无显著差异。覆膜前后物种分类显示，细菌种类均隶属于24门53纲112目218科322属，其中优势类群为蛋白菌门、厚壁菌门和放线菌门，覆膜前后的相对丰度分别为43.79%、21.70%、21.03%和42.36%、17.55%、23.92%。真菌种类均隶属于4门10纲21目27科34属，优势类群为子囊菌门和担子菌门，覆膜前后的相对丰度分别为62.48%、15.28%和51.13%、40.95%。可见，土壤中细菌群落的多样性和丰富度均高于真菌，覆膜前后微生物总量无显著变化，细菌、真菌在多样性指数上无显著差异，而在结构组成上差异显著。

关键词: 高温覆膜, 高通量测序, 韭菜迟眼蕈蚊, 土壤微生物多样性

Abstract: The aim of this study was to clear the effect of high temperature film mulching technology on soil microbial community in the rhizosphere of Chinese chive. The total DNA of Chinese chive rhizosphere soil before and after the high temperature film mulching were extracted, the 16Sr DNA of bacteria and ITS genes of fungal in the soil were sequenced by Illumina Hiseq 2500 high throughput sequencing, and the microbial community structure characteristics of the rhizosphere soil were analyzed. The results showed that the bacteria OTUs and the fungi OTUs before and after the high temperature film mulching were 1849 and 1819, 148 and 151, respectively, and, and there was no significant difference. According to the classification of species before and after the high temperature film mulching, the species of bacteria belonged to 24 phyla, 53 classes, 112 orders, 218 families and 322 genera. The dominant groups were Proteobacteria, Firmicutes and Actinobacteria, and their relative abundances were 43.79%, 21.03%, 21.70% and 42.36%, 23.92%, 17.55%, respectively. Fungal species belonged to 4 phyla, 10 classes, 21 orders, 27 families and 34 genera. The dominant species were Ascomycota and Basidiomycota, and their relative abundances were 62.48%, 15.28% and 51.13%, 40.95%, respectively. The results indicated that the diversity and richness of bacterial community in soil were higher than those of fungi. There was no significant change in total microbial biomass before and after mulching. There was no significant difference in diversity index between bacteria and fungi, but significant difference in structure composition.

Key words: high temperature film mulching, high throughput sequencing, Bradysia odoriphaga, soil microbial functional diversity

中图分类号:

S476.1

宋健, 张海剑, 刘莉, 杜立新, 柳健虎, 曹伟平. 高通量测序分析高温覆膜对韭菜根际微生物多样性的影响[J]. 中国生物防治学报, 2020, 36(6): 938-945.

SONG Jian, ZHANG Haijian, LIU Li, DU Lixin, LIU Jianhu, CAO Weiping. Effect of High Temperature Film Mulching on Soil Microbial Functional Diversity by High Throughput Sequencing[J]. Chinese Journal of Biological Control, 2020, 36(6): 938-945.

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高通量测序分析高温覆膜对韭菜根际微生物多样性的影响

Effect of High Temperature Film Mulching on Soil Microbial Functional Diversity by High Throughput Sequencing

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