一株氟啶草酮降解细菌的分离鉴定及降解特性研究

doi:10.16409/j.cnki.2095-039x.2026.02.044

中国生物防治学报 ›› 2026, Vol. 42 ›› Issue (4): 993-1005.DOI: 10.16409/j.cnki.2095-039x.2026.02.044

• 研究论文 • 上一篇

一株氟啶草酮降解细菌的分离鉴定及降解特性研究

李红^1,2,4, 牛新湘^3,5, 吴凤康^1,6, 盛月^1,4, 杨红梅^1,4,5, 楚敏^1,4,5, 包慧芳^1,4, 王宁^1,4,5, 詹发强^1,4, 杨蓉^1,4, 娄恺^1,4, 史应武^1,2,4,5

1. 新疆维吾尔自治区农业科学院微生物研究所, 乌鲁木齐 830091;
2. 新疆大学生命科学与技术学院, 乌鲁木齐 830046;
3. 新疆维吾尔自治区农业科学院农业资源与环境研究所, 乌鲁木齐 830091;
4. 新疆特殊环境微生物实验室, 乌鲁木齐 830091;
5. 农业农村部西北绿洲农业环境重点实验室, 乌鲁木齐 830091;
6. 新疆农业大学农学院, 乌鲁木齐 830052

收稿日期:2025-07-30 发布日期:2026-08-26
通讯作者: 史应武，博士，研究员，E-mail:syw1973@126.com。
作者简介:李红，女，硕士研究生，E-mail:2303276171@qq.com
基金资助:
自治区重点研发计划项目(2022B02053-2);国家重点研发计划项目(2022YFD1400304);国家重点研发计划项目(2021YFD1400205);自治区重大科技专项(2022A02005-3)

Isolation and Identification of a Fluridone-Degrading Strain and Its Degradation Characteristics

LI Hong^1,2,4, NIU Xinxiang^3,5, WU Fengkang^1,6, SHENG Yue^1,4, YANG Hongmei^1,4,5, CHU Min^1,4,5, BAO Huifang^1,4, WANG Ning^1,4,5, ZHAN Faqiang^1,4, YANG Rong^1,4, LOU Kai^1,4, SHI Yingwu^1,2,4,5

1. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
3. Institute of Agricultural Resources and Environment, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
4. Special Environment Microbiological Laboratory of Xinjiang, Urumqi 830091, China;
5. Key Laboratory of Northwest Oasis Agricultural Environment, Ministry of Agriculture and Rural Affairs, Urumqi 830091, China;
6. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China

Received:2025-07-30 Published:2026-08-26

摘要/Abstract

摘要： 本研究旨在利用微生物修复技术消减土壤氟啶草酮残留，缓解其对后茬作物的毒害效应。本研究以新疆长期施用氟啶草酮的棉田土壤为材料，通过富集培养法筛选获得可利用氟啶草酮为唯一碳源的降解菌株；结合形态观察、生理生化测定及16S rRNA基因序列分析完成菌株分类鉴定，并利用单因素试验探究其降解特性，同时借助盆栽试验验证菌株对小麦药害的缓解效果。结果表明，筛选得到的降解菌F9-1被鉴定为Acinetobacter oryzae。在氟啶草酮初始浓度100 mg/L条件下，该菌株最优降解参数为接种量5%、pH7.0、培养温度35℃；在此条件下培养7 d,F9-1对氟啶草酮的降解率可达79.62%。盆栽试验表明，菌株F9-1能够显著降低污染土壤中小麦幼苗叶片白化指数，提升叶片总叶绿素含量。综上，菌株F9-1丰富了氟啶草酮降解微生物资源，也为后续降解酶的挖掘与应用研究奠定了基础。

关键词: 氟啶草酮, 降解菌, 水稻不动杆菌, 生物降解, 降解特性

Abstract: This study aims to use microbial remediation technology to reduce soil fluridone residues and alleviate its toxic effects on subsequent crops. Using cotton field soil with long-term exposure to fluridone in Xinjiang, this study screened a degrading strain capable of utilizing fluridone as its sole carbon source via enrichment culture method. Combined with morphological observation, physiological and biochemical determination and 16S rRNA gene sequence analysis, the strain was classified and identified, and its degradation characteristics were explored by single factor test. At the same time, the mitigation effect of the strain on wheat phytotoxicity was verified by pot experiment. The results showed that the strain F9-1 was identified as Acinetobacter oryzae. Under the condition of initial concentration of fluridone 100 mg/L, the optimal degradation parameters of the strain were inoculation amount of 5%, pH 7.0, and culture temperature of 35°C. Under these conditions, the degradation rate of fluridone could reach 79.62% after 7 days of culture. Pot experiments confirmed that the inoculation strain F9-1 could significantly reduce the albino index of wheat seedlings in contaminated soil and increase the total chlorophyll content of leaves. In summary, strain F9-1 enriched the microbial resources for fluridone degradation, and also laid a foundation for the subsequent excavation and application of degrading enzymes.

Key words: fluridone, degrading bacteria, Acinetobacter oryzae, biodegradation, degradation characteristics

中图分类号:

S476

李红, 牛新湘, 吴凤康, 盛月, 杨红梅, 楚敏, 包慧芳, 王宁, 詹发强, 杨蓉, 娄恺, 史应武. 一株氟啶草酮降解细菌的分离鉴定及降解特性研究[J]. 中国生物防治学报, 2026, 42(4): 993-1005.

LI Hong, NIU Xinxiang, WU Fengkang, SHENG Yue, YANG Hongmei, CHU Min, BAO Huifang, WANG Ning, ZHAN Faqiang, YANG Rong, LOU Kai, SHI Yingwu. Isolation and Identification of a Fluridone-Degrading Strain and Its Degradation Characteristics[J]. Chinese Journal of Biological Control, 2026, 42(4): 993-1005.

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参考文献

[1] 秦培元,哈小菲,唐子人,等.新疆哈密棉田杂草组成及群落结构分析[J]. 新疆农业科技, 2024(1):36-39.
[2] 孙利忠,马保建,刘彤.新疆北疆滴灌机采棉田杂草生态位机理研究[J]. 棉花学报, 2024, 36(1):66-78.
[3] 郭世俭,章振,赵东,等. 42%氟啶草酮悬浮剂滴施防治新疆棉田杂草研究[J]. 中国棉花, 2020, 47(9):11-16.
[4] 马明亮,张卓亚,吴靖涛,等.氟啶草酮与扑草净混用防除棉田杂草应用技术[J]. 农药, 2023, 62(10):777-780.
[5] VAN Frost S R, White A M, Jauquet J M, et al. Laboratory measurements underestimate persistence of the aquatic herbicide fluridone in lakes[J]. Environmental Science:Processes & Impacts, 2024, 26(2):368-379.
[6] 赵冰梅,丁丽丽,张强,等. 42%氟啶草酮悬浮剂桶混二甲戊灵对覆膜棉田恶性杂草防除效果及安全性[J]. 中国棉花, 2018, 45(2):33-36,43.
[7] Zou Z, Zou X, Zhao S, et al. Fluridone induces leaf bleaching by inhibiting pigment biosynthesis via downregulated transcription levels of pigment biosynthetic genes in rice(Oryza sativa L.)[J]. Journal of Plant Growth Regulation, 2018, 37(4):1385-1395.
[8] 赵霞,夏丽娟,李婷,等. 42%氟啶草酮悬浮剂对棉花后茬作物的安全性[J]. 农药, 2021, 60(12):897-899.
[9] Schroeder J, Banks P A. Persistence and activity of norflurazon and fluridone in five georgia soils under controlled conditions[J]. Weed Science, 1986,34(4):599-606.
[10] Elsie I H, Mohammed K H. Residual fluridone in humid tropical soils:Carryover effects on germination and seedling growth of maize(Zea mays L.)[J]. Resources and Environment, 2018, 8(2):5-10.
[11] Hill Z T, Norsworthy J K, Barber L T, et al. Assessing the potential for fluridone carryover to six crops rotated with Ccotton[J]. Weed Technology, 2016,30(2):346-354.
[12] 郭世俭,赵东,周国栋,等. 42%氟啶草酮悬浮剂桶混二甲戊灵对7种后茬作物的安全性研究[J]. 中国棉花, 2020, 47(4):14-17.
[13] 王哲,屠春宝,王如月等.农业土壤环境污染及修复研究进展[J]. 农业与技术, 2023, 43(19):94-99.
[14] 杨霞,荆常亮,余佳敏,等.农药残留降解技术研究现状[J]. 安徽农业科学, 2023, 51(2):24-26, 36.
[15] West S D, Burger R O. Gas chromatographic determination of fluridone aquatic herbicide and its major metabolite in fish[J]. Journal of Association of Official Analytical Chemists, 1980, 63(6):1304-1309.
[16] West S D, Day E W Jr. Liquid chromatographic determination of fluridone aquatic herbicide and its metabolite in fish and crayfish[J]. Journal of Association of Official Analytical Chemists, 1986, 69(5):856-859.
[17] West SD, Turner LG. Residue level determination of the aquatic herbicide fluridone and a potential photoproduct(N-methylformamide)in water[J]. Journal of Association of Official Analytical Chemists, 1988, 71(5):1049-1053.
[18] 冯娟,杨凯淇,王高红,等.土壤生物修复技术的研究现状与发展[J]. 陕西农业科学, 2023, 69(3):104-109.
[19] 雷向荣.氟咯草酮降解菌株的筛选及其降解效果的研究[D]. 西宁:青海大学, 2022.
[20] 雷向荣,沈硕,李玮. 3株氟咯草酮降解菌株的筛选及其生物降解效果研究[J]. 中国生物防治学报, 2021, 37(6):1231-1240.
[21] Książek-Trela P, Bielak E, Węzka D, et al. Effect of three commercial formulations containing effective microorganisms(EM)on diflufenican and flurochloridone degradation in soil[J]. Molecules, 2022, 27(14):4541.
[22] 颜宇.虫砂及其复配降解菌缓解氟啶草酮及二甲戊灵对小麦残留药害效果[D]. 乌鲁木齐:新疆农业大学, 2024.
[23] 山东省农药科学研究院.一种氟啶草酮残留量的HPLC-MS/MS测定方法:CN201810818911.3[P]. 2018-12-07.
[24] 濮文均,孙均燕,戴宝江.氟啶草酮原药的高效液相色谱分析[J]. 精细化工中间体, 2013, 43(6):71-73.
[25] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[26] 王玉威,张李婷,徐敏,等.一株聚乙烯塑料降解细菌的分离、鉴定及其降解特性[J]. 生物工程学报, 2025, 41(6):2405-2414.
[27] 伍强强,房欢欢,李小虎,等.不同土壤封闭除草剂对麦田阔叶杂草的防效研究[J]. 现代农业科技, 2022,(24):83-85, 94.
[28] 章振,郭世俭,张卓亚,等.氟啶草酮对新疆当地常见作物的安全性研究[J]. 世界农药, 2021, 43(12):38-41+49.
[29] Cahoon C W, York A C, Jordan D L, et al. Fluridonecarryover to rotational crops following application to cotton[J]. Journal of Cotton Science,2015, 19:631-640.
[30] 秦胜红,李新宇,李旭,等.不同土壤细菌种群结构对氯嘧磺隆胁迫的响应及降解菌系的获得[J]. 生态学杂志, 2012, 31(7):1724-1732.
[31] Yang R, Wang S, Yousaf G, et al. Bioremediation of fluridone by Acinetobacter bereziniae FJ-5:Metabolic pathway, metabolomics mechanism and biotoxicity evaluation[J]. Journal of Hazardous Materials, 2026, 496:141160.
[32] Książek-Trela P, Potocki L, Szpyrka E. The impact of novel bacterial strains and their consortium on diflufenican degradation in the mineral medium and soil[J]. Scientific Reports. 2025, 15(1):18051.
[33] Pinto A P, Rodrigues S C, Caldeira A T, et al. Exploring the potential of novel biomixtures and Lentinula edodes fungus for the degradation of selected pesticides. Evaluation for use in biobed systems[J]. Science of The Total Environment, 2016, 541:1372-1381.
[34] Rouchaud J, Neus O, Callens D, et al. Herbicide flurochloridone soil biodegradation in potato crops[J]. Toxicological & Environmental Chemistry, 1997,61(1-4):251-257.
[35] Pirog T P, Lutsai D A, Muchnyk F V. Biotechnological potential of the Acinetobacter genus bacteria[J]. Microbiological Journal, 2021, 83(3):92-109.
[36] Huang X, He J, Yan X, et al. Microbial catabolism of chemical herbicides:microbial resources, metabolic pathways and catabolic genes[J]. Pesticide Biochemistry and Physiology, 2017, 143:272-297.
[37] Wu Q, Li F, Zhu X, et al. Isolation and characterization of cyromazine degrading Acinetobacter sp. ZX01 from a Chinese ginger cultivated soil[J]. Environmental Science and Pollution Research, 2022, 29(45):67765-67775.
[38] West S D, Day Jr E W, Burger R O. Dissipation of the experimental aquatic herbicide fluridone from lakes and ponds[J]. Journal of Agricultural and Food Chemistry, 1979, 27(5):1067-1072.
[39] Chen F, Wu C, Wang Y, et al. Phytotoxicity of fluridone and emerging transformation products in agricultural soils:Insights into molecular interactions and photosynthetic disruptions in maize[J]. Journal of Hazardous Materials, 2025, 495:139252.
[40] 郭世俭,赵东,周国栋,等. 42%氟啶草酮悬浮剂桶混二甲戊灵对7种后茬作物的安全性研究[J]. 中国棉花, 2020, 47(4):14-17.
[41] Hill Z T, Norsworthy J K, Barber L T, et al. Assessing the potential for fluridone carryover to six crops rotated with cotton[J]. Weed Technology, 2016,30(2):346-354.
[42] He P, Xing Z, Zhao T, et al. Are microbial consortia superior? A systematic quantitative assessment of biological traits and multi-pollutant degradation under comparable experimental contexts[J]. Environmental Microbiology, 2026, 28(7):e70370.

一株氟啶草酮降解细菌的分离鉴定及降解特性研究

Isolation and Identification of a Fluridone-Degrading Strain and Its Degradation Characteristics

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