温度对胡瓜新小绥螨生殖力及钠钾泵α亚基基因表达的影响

doi:10.16409/j.cnki.2095-039x.2016.05.002

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (5): 562-568.DOI: 10.16409/j.cnki.2095-039x.2016.05.002

温度对胡瓜新小绥螨生殖力及钠钾泵α亚基基因表达的影响

赵云龙^1,2,3, 李敦松¹, 张敏², 陈威², 张古忍²

1. 广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640;
2. 中山大学昆虫研究所/有害控制与资源利用国家重点实验室, 广州 510275;
3. 通化师范学院, 通化 134002

收稿日期:2016-02-26 出版日期:2016-10-08 发布日期:2016-10-08
通讯作者: 李敦松, 张古忍
作者简介:赵云龙,博士研究生,E-mail:252268020@qq.com
基金资助:
国家公益性行业（农业）科研专项（200903032）；国家“973”计划（2013CB127602）；广东省科技计划项目（2015A020209071）

Effects of Temperature on the Fertility and the Expression of Na⁺/K⁺-ATPase α Subunit of the Biological Control Agent, Neoseiulus cucumeris (Oudemans)

ZHAO Yunlong^1,2,3, LI Dunsong¹, ZHANG Min², CHEN Wei², ZHANG Guren²

1. Guangdong Provincial Key Laboratory of High Technology for Plant Protection/Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. State Key Laboratory for Biocontrol/Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, China;
3. Tonghua Normal University, Tonghua 134002, China

Received:2016-02-26 Online:2016-10-08 Published:2016-10-08

摘要/Abstract

摘要： 胡瓜新小绥螨Neoseiulus cucumeris（Oudemans）广泛地用于蓟马类害虫的防治、控制及管理。本研究克隆了1条胡瓜新小绥螨钠钾泵α亚基基因序列，分析了温度对雌螨生殖力及钠钾泵α亚基基因表达的影响。在15、20、25和30℃下，胡瓜新小绥螨在25℃时生殖力最优。在上述温度条件下胁迫48 h，雌螨钠钾泵α亚基在15和30℃下表达量显著上升，能量代谢活跃。表达量越多，表明能量消耗越大，投入生殖的能量就越少，结合生殖力研究结果，二者一致。高温或低温均会降低胡瓜新小绥螨的生殖力，且高温影响更为严重。

关键词: 胡瓜新小绥螨, 温度, 生殖力, 钠钾泵α亚基

Abstract: Neoseiulus cucumeris (Oudemans) is widely used as a biological control agent for some insect pests. The effects of temperature on the fertility and the expression of Na⁺/K⁺-ATPase α subunit based on the clone of Na⁺/K⁺-ATPase α subunit gene of female adults were assessed in the present paper. The results show that 25℃ is the optimal temperature for female fertility among a temperature series of 15, 20, 25 and 30℃. Expression of Na⁺/K⁺-ATPase α subunit gene increased significantly in females stressed at 15 and 30℃ for 48 h, indicating that energy metabolism in stressed females was more active than in females at optimal temperature. The results suggest that females would spend less energy for fertility if they were stressed with high or low temperature.

Key words: Neoseiulus cucumeris, temperature, fertility, Na⁺/K⁺-ATPase α subunit

中图分类号:

S476.2

赵云龙, 李敦松, 张敏, 陈威, 张古忍. 温度对胡瓜新小绥螨生殖力及钠钾泵α亚基基因表达的影响[J]. 中国生物防治学报, 2016, 32(5): 562-568.

ZHAO Yunlong, LI Dunsong, ZHANG Min, CHEN Wei, ZHANG Guren. Effects of Temperature on the Fertility and the Expression of Na⁺/K⁺-ATPase α Subunit of the Biological Control Agent, Neoseiulus cucumeris (Oudemans)[J]. journal1, 2016, 32(5): 562-568.

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温度对胡瓜新小绥螨生殖力及钠钾泵α亚基基因表达的影响

Effects of Temperature on the Fertility and the Expression of Na⁺/K⁺-ATPase α Subunit of the Biological Control Agent, Neoseiulus cucumeris (Oudemans)

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温度对胡瓜新小绥螨生殖力及钠钾泵α亚基基因表达的影响

Effects of Temperature on the Fertility and the Expression of Na+/K+-ATPase α Subunit of the Biological Control Agent, Neoseiulus cucumeris (Oudemans)

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Effects of Temperature on the Fertility and the Expression of Na⁺/K⁺-ATPase α Subunit of the Biological Control Agent, Neoseiulus cucumeris (Oudemans)