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中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (5): 644-649.DOI: 10.16409/j.cnki.2095-039x.2017.05.010

• 研究论文 • 上一篇    下一篇

丁香假单胞菌对亚洲玉米螟幼虫抗寒能力的影响

阿依谢姆古丽·亚库普1, 安尼瓦尔·库尔班1, 郭文超2   

  1. 1. 新疆农业大学农学院, 乌鲁木齐 830052;
    2. 新疆农业科学院植物保护研究所, 乌鲁木齐 830091
  • 收稿日期:2017-03-29 出版日期:2017-10-08 发布日期:2017-10-11
  • 通讯作者: 安尼瓦尔·库尔班
  • 作者简介:阿依谢姆古丽·亚库普,E-mail:2538627821@qq.com;安尼瓦尔·库尔班,博士,副教授,E-mail:1834238969@qq.com。
  • 基金资助:
    国家自然科学基金(31260428)

Effect of Pseudomonas syringae on Cold Hardiness of Ostrinia furnacalis Larvae

AYXEMGVL Yakup1, ANWAR Kurban1, GUO Wenchao2   

  1. 1. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2017-03-29 Online:2017-10-08 Published:2017-10-11

摘要: 本文用丁香假单胞菌Pseudomonas syringae处理亚洲玉米螟Ostrinia furnacalis(Guenée)幼虫,使用过冷却仪测定过冷却点,分析该菌株对参试幼虫耐寒能力的影响。结果表明,亚洲玉米螟5龄非滞育幼虫抗寒能力比其他龄期非滞育幼虫强。亚洲玉米螟滞育5龄幼虫抗寒能力比非滞育5龄幼虫强。使用丁香假单胞菌处理亚洲玉米螟5龄非滞育和滞育幼虫,24 h后非滞育幼虫的过冷却点和结冰点分别提高了5.14和2.83℃。5龄滞育幼虫过冷却点和结冰点分别提高了11.25和4.65℃。丁香假单胞菌菌悬液(浓度为8×108 cfu/mL)对亚洲玉米螟具有较好的杀虫效果,非滞育5龄幼虫在(15±1)℃条件下处理24 h后平均死亡率39.1%。

关键词: 亚洲玉米螟, 过冷却点, 低温驯化, 丁香假单胞菌, 抗寒能力

Abstract: Effect of Pseudomonas syringae on cold tolerance of the Asian corn borer larvae, Ostrinia furnacalis (Guenée), was analyzed by measureing the supercooling point. Results indicated that cold resistance of the non diapause 5th instar O. furnacalis larvae was stronger than that of other age groups. The cold resistance of the 5th instar diapause O. furnacalis larvae was higher than that of non diapause 5th instar larvae. When treated with Pseudomonas syringae for 24 h, the supercooling point and freezing point of non diapause 5th instar O. furnacalis larvae increased by 5.14℃ and 2.83℃, respectively; and diapause 5th instar larvae increased by 11.46℃ and 4.65℃, respectively. P. syringae (at concentration of 8×108 cfu/ml) had a good insecticidal effect on O. furnacalis, with mortality rate of non-diapause 5th instar larvae being 39.1% under 15℃ after 24 h.

Key words: Ostrinia furnacalis, supercooling point, cold acclimation, Pseudomonas Syringae, cold tolerance

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