两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较

doi:10.16409/j.cnki.2095-039x.2017.06.002

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (6): 726-731.DOI: 10.16409/j.cnki.2095-039x.2017.06.002

• 生物农药与化学农药协同作用专栏 • 上一篇下一篇

两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较

许国升¹, 王翠翠¹, 张贤^2,3, 束长龙², 李振宇⁴, 史雪岩¹, 张杰²

1. 中国农业大学植物保护学院, 北京 100193;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
3. 山西师范大学, 临汾 041004;
4. 广东省农业科学院植物保护研究所, 广州 510640

收稿日期:2016-10-26 出版日期:2017-12-08 发布日期:2017-12-16
通讯作者: 史雪岩,博士,副教授,E-mail:shixueyan@cau.edu.cn
作者简介:许国升,硕士研究生,E-mail:guoshengx@126.com
基金资助:
国家重点研发计划（2017YFD0201204）

Comparison of Susceptibilities to Cry1Ac between Two Populations of Plutella xylostella with Different Resistance Backgrounds to Chemical Insecticides

XU Guosheng¹, WANG Cuicui¹, ZHANG Xian^2,3, SHU Changlong², LI Zhenyu⁴, SHI Xueyan¹, ZHANG Jie²

1. College of Plant Protection, China Agricultural University, Beijing 100193, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Shanxi Normal University, Linfen 041004, China;
4. Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received:2016-10-26 Online:2017-12-08 Published:2017-12-16

摘要/Abstract

摘要： 为在小菜蛾防治中合理使用化学杀虫剂和生物农药Bt，探究了小菜蛾对化学农药的抗药性与其对Bt杀虫蛋白Cry1Ac的敏感度之间的关系。采用浸叶法测定了2个抗性背景不同的小菜蛾种群对9种常用杀虫剂和Cry1Ac蛋白的敏感度，通过比较敏感度差异，初步探索了小菜蛾对化学农药的抗药性和其对Cry1Ac蛋白敏感度之间的关系。结果表明：采自广东博罗县田间的小菜蛾种群（GDBL）对除Cry1Ac之外的9种常用杀虫剂，均已产生了不同程度的抗药性；而北京室内小菜蛾种群（BJNK），虽然对高效氯氰菊酯、阿维菌素、茚虫威、多杀菌素、溴虫腈具有抗性，对Cry1Ac在内的其他5种杀虫剂仍比较敏感。可见，对多种不同类型的杀虫剂产生了不同程度抗性的两个小菜蛾种群，对Cry1Ac蛋白仍处于敏感状态。由于小菜蛾对化学杀虫剂的抗性机制与其对Cry1Ac的抗性机制不同，小菜蛾对化学杀虫剂的抗药性与其对Cry1Ac的抗药性之间不存在交互抗性。因此，在田间可以通过加强Bt杀虫剂的使用来提高对多种化学杀虫剂产生抗性的小菜蛾的防治效果。

关键词: 小菜蛾, 化学农药, 生物农药, BtCry1Ac蛋白, 抗药性

Abstract: For rational use of chemical insecticides and bio-pesticides Bt in the control of Plutella xylostella, the relationship between the resistance of P. xylostella to chemical insecticides and susceptibility to Bt Cry1Ac protein was investigated. The susceptibilities of two P. xylostella populations with different resistance backgrounds to 9 commonly used insecticides and Cry1Ac protein were determined using leaf-dipping method and the cross-resistance of the two P. xylostella populations to chemical insecticides and Cry1Ac protein was clarified. The results showed that the GDBL population, collected from fields in Boluo County, Guangdong province, exhibited different resistance levels to the nine commonly used insecticides, except to Cry1Ac protein. Though the laboratory population BJNK showed resistance to beta-cypermethrin, abamectin, indoxacarb, spinosadand chlorfenapyr, BJNK population is still susceptible to the other five insecticides including Cry1Ac protein. Our results showed that the two P. xylostella populations of different resistance backgrounds to several insecticides are still susceptible to Cry1Ac protein. No cross-resistance was observed between the resistance to chemical insecticides and that to Cry1Ac protein, because the resistance mechanisms to chemical insecticides are different from that to Cry1Ac protein. Therefore, Bt can be used for effectively control of field P. xylostella populations that have already exhibited resistance to a variety of chemical insecticides.

Key words: Plutella xylostella, chemical insecticides, biological insecticide, Bt Cry1Ac protein, resistance

中图分类号:

S476.1

许国升, 王翠翠, 张贤, 束长龙, 李振宇, 史雪岩, 张杰. 两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较[J]. 中国生物防治学报, 2017, 33(6): 726-731.

XU Guosheng, WANG Cuicui, ZHANG Xian, SHU Changlong, LI Zhenyu, SHI Xueyan, ZHANG Jie. Comparison of Susceptibilities to Cry1Ac between Two Populations of Plutella xylostella with Different Resistance Backgrounds to Chemical Insecticides[J]. journal1, 2017, 33(6): 726-731.

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两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较

Comparison of Susceptibilities to Cry1Ac between Two Populations of Plutella xylostella with Different Resistance Backgrounds to Chemical Insecticides

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