假单胞菌产生的抗生素

doi:10.16409/j.cnki.2095-039x.2015.05.015

摘要/Abstract

摘要： 假单胞菌属Pseudomonas群体庞大,广泛分布于土壤、水体和动植物中,并产生大量的次生代谢产物以适应环境的变化。抗生素为假单胞菌在自然生态条件下的生存提供了重要的竞争手段,同时也是生防假单胞菌防治植物病害的主要机制之一。本文介绍假单胞菌产生的抗生素种类、作用机制、不同浓度下抗生素的生物学功能,以及假单胞菌抗生素在医学和农业病害防治中的应用。

关键词: 假单胞菌, 抗生素, 生物农药, 信号

Abstract: Pseudomonas is a large genus known for its wide spreading in natural habitats, including soil, water, and its association with plants and animals. Members of Pseudomonas genus produce a long list of secondary metabolites during their adaption of environmental changes. Antibiotics produced by Pseudomonas spp. facilitate bacterial colonization in the competition against the indigenous microbial community, and function as major components for biocontrol agents to suppress plant diseases in agricultural environments. This article introduces the characteristics and action model of antibiotics produced by Pseudomonas, their biological functions in different concentrations, and the commercial application of these antibiotics in agriculture and medicine.

Key words: Pseudomonas, antibiotics, biological pesticide, signal

中图分类号:

S476
Q93

张力群, 张俊威. 假单胞菌产生的抗生素[J]. 中国生物防治学报, 2015, 31(5): 750-756.

ZHANG Liqun, ZHANG Junwei. Antibiotics Produced by Pseudomonas spp.[J]. journal1, 2015, 31(5): 750-756.

参考文献

[1] Gross H, Loper J E. Genomics of secondary metabolite production by Pseudomonas spp.[J]. Natural Product Reports, 2009, 26(11): 1408-1446.
[2] Wu X, Monchy S, Taghavi S, et al. Comparative genomics and functional analysis of niche-specific adaptation in Pseudomonas putida[J]. FEMS Microbiology Reviews, 2010, 35(2): 299-323.
[3] Loper J E, Hassan K A, Mavrodi D V, et al. Comparative genomics of plant-associated Pseudomonas spp.: insights into diversity and inheritance of traits involved in multitrophic interactions[J]. PLoS Genetics, 2012, 8(7): e1002784.
[4] Haas D, Defago G. Biological control of soil-borne pathogens by fluorescent pseudomonads[J]. Nature Reviews Microbiology, 2005, 3(4): 307-319.
[5] Stockwell V O, Stack J P. Using Pseudomonas spp. for integrated biological control[J]. Phytopathology, 2007, 97(2): 244-249.
[6] Brucker R M, Baylor C M, Walters R L, et al. The identification of 2,4-diacetylphloroglucinol as an antifungal metabolite produced by cutaneous bacteria of the salamander Plethodon cinereus[J]. Journal of Chemical Ecology, 2008, 34(1): 39-43.
[7] Weller D M, Landa B B, Mavrodi O V, et al. Role of 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. in the defense of plant roots[J]. Plant Biology, 2007, 9(1): 4-20.
[8] Weller D M, Raaijmakers J M, McSpadden Gardener B B, et al. Microbial populations responsible for specific soil suppressiveness to plant pathogens[J]. Annual Review of Phytopathology, 2002, 40: 309-348.
[9] Zhang L Q, Yang Q, Tosa Y, et al. Involvement of gacA gene in the suppression of tomato bacterial wilt by Pseudomonas fluorescens FPT9601[J]. Journal of General Plant Pathology, 2001, 67(2): 134-143.
[10] de Souza J T, Arnould C, Deulvot C, et al. Effect of 2,4-diacetylphloroglucinol on Pythium: cellular responses and variation in sensitivity among propagules and species[J]. Phytopathology, 2003, 93(8): 966-975.
[11] Keel C, Schnider U, Mauurhofer M, et al. Suppression of root diseases by Pseudomonas fluorescens CHA0: importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol[J]. Molecular Plant-Microbe Interactions, 1992, 5(1): 4-13.
[12] Troppens D M, Dmitriev R I, Papkovsky D B, et al. Genome-wide investigation of cellular targets and mode of action of the antifungal bacterial metabolite 2,4-diacetylphloroglucinol in Saccharomyces cerevisiae[J]. FEMS Yeast Research, 2013, 13(3): 322-334.
[13] Kwak Y S, Han S, Thomashow L S, et al. Saccharomyces cerevisiae genome-wide mutant screen for sensitivity to 2,4-diacetylphloroglucinol, an antibiotic produced by Pseudomonas fluorescens[J]. Applied and Environmental Microbiology, 2011, 77(5): 1770-1776.
[14] Laursen J B, Nielsen J. Phenazine natural products: biosynthesis, synthetic analogues, and biological activity[J]. Chemical Reviews, 2004, 104: 1663-1686.
[15] Pierson L S, Thomashow L S. Cloning and heterologous expression of the phenazine biosynthetic locus from Pseudomonas aureofaciens 30-84[J]. Molecular Plant-Microbe Interactions, 1992, 5(4): 330-339.
[16] Chin-A-Woeng T F C, Bloemberg G V, van der Bij A J, et al. Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici[J]. Molecular Plant-Microbe Interactions, 1998, 11(11): 1069-1077.
[17] Pierson L S, Pierson E A. Metabolism and function of phenazines in bacteria: impacts on the behavior of bacteria in the environment and biotechnological processes[J]. Applied Microbiology and Biotechnology, 2010, 86(6): 1659-1670.
[18] Arima K, Imanaka I, Kousaka M, et al. Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas[J]. Agricultural and Biological Chemistry, 1964, 28: 575-576.
[19] Selin C, Habibian R, Poritsanos N, et al. Phenazines are not essential for Pseudomonas chlororaphis PA23 biocontrol of Sclerotinia sclerotiorum, but do play a role in biofilm formation[J]. FEMS Microbiology Ecology, 2010, 71(1): 73-83.
[20] Hill D S, Stein J I, Torkewitz N R, et al. Cloning of genes involved in the synthesis of pyrrolnitrin from Pseudomonas fluorescens and role of pyrrolnitrin synthesis in biological control of plant disease[J]. Applied and Environmental Microbiology, 1994, 60(1): 78-85.
[21] Tripathi R K, Gottlieb D. Mechanism of action of antifungal antibiotic pyrrolnitrin[J]. Journal of Bacteriology, 1969, 100(1): 310-318.
[22] Pillonel C, Meyer T. Effect of phenylpyrroles on glycerol accumulation and protein kinase activity of Neurospora crassa[J]. Pesticide Science, 1997, 49(3): 229-236.
[23] Okada A, Banno S, Ichiishi A, et al. Pyrrolnitrin interferes with osmotic signal transduction in Neurospora crassa[J]. Journal of Pesticide Science, 2005, 30: 378-383.
[24] Ligon J M, Hill D S, Hammer P E, et al. Natural products with antifungal activity from Pseudomonas biocontrol bacteria[J]. Pest Management Science, 2000, 56(8): 688-695.
[25] Partida-Martinez L P, Hertweck C. Pathogenic fungus harbours endosymbiotic bacteria for toxin production[J]. Nature, 2005, 437(6): 884-888.
[26] Loper J E, Henkels M D, Shaffer B T, et al. Isolation and identification of rhizoxin analogs from Pseudomonas fluorescens Pf-5 by using a genomic mining strategy[J]. Applied and Environmental Microbiology, 2008, 74(10): 3085-3093.
[27] Takeuchi K, Noda N, Katayose Y, et al. Rhizoxin analogs contribute to the biocontrol activity of a newly isolated Pseudomonas strain[J]. Molecular Plant-Microbe Interactions, 2015, 28(3): 333-342.
[28] Takahashi M, Matsumoto S, Iwasaki S, et al. Molecular basis for determining the sensitivity of eucaryotes to the antimitotic drug rhizoxin[J]. Molecular and General Genetics, 1990, 222(2-3): 169-175.
[29] Fuller A T, Mellows G, Woolford M, et al. Pseudomonic acid: an antibiotic produced by Pseudomonas fluorescens[J]. Nature, 1971, 234(5329): 416-417.
[30] Yanagisawa T, Lee J T, Wu H C, et al. Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli. A proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-tRNA synthetase[J]. Journal of Biological Chemistry, 1994, 269(39): 24304-24309.
[31] Hughes J, Mellows G, Soughton S. How does Pseudomonas fluorescens, the producing organism of the antibiotic pseudomonic acid A, avoid suicide?[J]. FEBS Letters, 1980, 122(2): 322-324.
[32] Howell C R, Stipanovic R. D. Suppression of Pythium ultimum-induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin[J]. Phytopathology, 1980, 70(8): 712-715.
[33] Cazorla F M, Duckett S, Bergström E, et al. Biocontrol of avocado Dematophora root rot by antagonistic Pseudomonas fluorescens PCL1606 correlates with the production of 2-hexyl, 5-propyl resorcinol[J]. Molecular Plant-Microbe Interactions, 2006, 19(4): 418-428.
[34] Kaur R, Macleod J, Foley W, et al. Gluconic acid: an antifungal agent produced by Pseudomonas species in biological control of take-all[J]. Phytochemistry, 2006, 67(6): 595-604.
[35] de Werra P, Péchy-Tarr M, Keel C, et al. Role of gluconic acid production in the regulation of biocontrol traits of Pseudomonas fluoresce CHA0[J]. Applied and Environmental Microbiology, 2009, 75(12): 4162-4174.
[36] van de Mortel J E, Tran H, Govers F, et al. Cellular responses of the late blight pathogen Phytophthora infestans to cyclic lipopeptide surfactants and their dependence on G proteins[J]. Applied and Environmental Microbiology, 2009, 75(15): 4950-4957.
[37] Raaijmakers J M, de Bruijn I, Nybroe O, et al. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics[J]. FEMS Microbiology Reviews, 2010, 34(6): 1037-1062.
[38] Ramette A, Moenne-Loccoz Y, Defago G. Prevalence of fluorescent pseudomonads producing antifungal phloroglucinols and/or hydrogen cyanide in soils naturally suppressive or conducive to tobacco black root rot[J]. FEMS Microbiology Ecology, 2003, 44(1): 35-43.
[39] Dietrich L E, Price-Whelan A, Petersen A, et al. The phenazine pyocyanin is a terminal signaling factor in the quorum sensing network of Pseudomonas aeruginosa[J]. Molecular Microbiology, 2006, 61(5): 1308-1321.
[40] Combes-Meynet E, Pothier J F, Moenne-Loccoz Y, et al. The Pseudomonas secondary metabolite 2,4-diacetylphloroglucinol is a signal inducing rhizoplane expression of Azospirillum genes involved in plant-growth promotion[J]. Molecular Plant-Microbe Interactions, 2011, 24: 271-284.
[41] Powers M J, Sanabria-Valentín E, Bowers A A, et al. Inhibition of cell differentiation in Bacillus subtilis by Pseudomonas protegens[J]. Journal of Bacteriology, 2015, 197(13): 2129-2138.
[42] Weller D M, Mavrodi D V, van Pelt J A, et al. Induced systemic resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato by 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens[J]. Phytopathology, 2012, 102(4): 403-412.
[43] Iavicoli A, Boutet E, Buchala A, et al. Induced systemic resistance in Arabidopsis thaliana in response to root inoculation with Pseudomonas fluorescens CHA0[J]. Molecular Plant-Microbe Interactions, 2003, 16(10): 851-858.
[44] de Vleesschauwer D, Cornelis P, Höfte M. Redox-active pyocyanin secreted by Pseudomonas aeruginosa 7NSK2 triggers systemic resistance to Magnaporthe grisea but enhances Rhizoctonia solani susceptibility in rice[J]. Molecular Plant-Microbe Interactions, 2006, 19(12): 1406-1419.
[45] Ligon J M, Hill D S, Hammer P E, et al. Natural products with antifungal activity from Pseudomonas biocontrol bacteria[J]. Pest Management Science, 2000, 56(8): 688-695.
[46] 方运玲, 孙爽, 申阅, 等. 微生物源农药申嗪霉素的研制与应用[J]. 农药学学报, 2014, 16(4): 387-393.
[47] Ge Y, X. Huang S, Wang X, et al. Phenazine-1-carboxylic acid is negatively regulated and pyoluteorin positively regulated by gacA in Pseudomonas sp. M18[J]. FEMS Microbiology Letters, 2004, 237(1): 41-47.
[48] Hurdle J G, O'Neill A J, Chopra I. Prospects for aminoacyl-tRNA synthetase inhibitors as new antimicrobial agents[J]. Antimicrobial Agents and Chemotherapy, 2005, 49(12): 4821-4833.
[49] Sutherland R, Boon R J, Griffin K E, et al. Antibacterial activity of mupirocin (pseudomonic acid), a new antibiotic for topical use[J]. Antimicrobial Agents and Chemotherapy, 1985, 27(4): 495-498.
[50] Mendes R, Kruijt M, de Bruijn I, et al. Deciphering the rhizosphere microbiome for disease-suppressive bacteria[J]. Science, 2011, 332(6033): 1097-1100.
[51] Stallforth P, Brock D, Cantley A M, et al. A bacterial symbiont is converted from an inedible producer of beneficial molecules into food by a single mutation in the gacA gene[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(36): 14528-14533.