不同Bt杀虫蛋白通过食物链对大草蛉的影响

doi:10.16409/j.cnki.2095-039x.2019.04.003

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (4): 557-562.DOI: 10.16409/j.cnki.2095-039x.2019.04.003

不同Bt杀虫蛋白通过食物链对大草蛉的影响

赵曼^1,2, 汤金荣², 牛琳琳¹, 陈琳¹, 梁革梅¹

1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
2. 河南农业大学植物保护学院, 郑州 450002

收稿日期:2019-02-21 出版日期:2019-08-08 发布日期:2019-08-10
通讯作者: 梁革梅,博士,研究员,E-mail:gmliang@ippcaas.cn。
作者简介:赵曼,博士,讲师,E-mail:zhaoman821@126.com
基金资助:
国家转基因生物新品种培育重大专项（2016ZX08011-002）

Tritrophic Effects of Different Bt Insecticidal Proteins on Chrysopa pallens

ZHAO Man^1,2, TANG Jinrong², NIU Linlin¹, CHEN Lin¹, LIANG Gemei¹

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China

Received:2019-02-21 Online:2019-08-08 Published:2019-08-10

摘要/Abstract

摘要： 为了明确目前转基因抗虫植物中表达的Bt蛋白对农田重要捕食性天敌大草蛉的生态安全性，采用三级营养食物链评价体系，分别以对供试Bt蛋白Cry1Ac、Cry1F和Cry2Ab具有抗性的靶标害虫或不敏感的非靶标害虫作为大草蛉的猎物，研究了供试3种蛋白对大草蛉的影响。结果表明，当大草蛉以抗性棉铃虫（抗Cry1Ac或Cry2Ab）或抗性玉米螟（抗Cry1F）为猎物时，供试3种Bt蛋白通过食物链对大草蛉幼虫（4龄幼虫重量和幼虫发育至蛹历期）、蛹（化蛹率、蛹重和蛹发育历期）及成虫（羽化率、雌虫重量、雄虫重量和单雌产卵量）的生长发育及产卵量均无显著影响；同样地，当大草蛉以对3种Bt蛋白不敏感的非靶标昆虫——绿盲蝽作为猎物时，通过食物链传递的Bt蛋白对大草蛉生长发育及繁殖也无显著影响。因此，目前转基因棉花、玉米或水稻中广泛表达的Cry1Ac、Cry1F和Cry2Ab蛋白，通过三级营养食物链，对大草蛉生长发育及繁殖均无潜在毒性。

关键词: Bt杀虫蛋白, 食物链, 大草蛉, 转基因抗虫植物

Abstract: In order to clarify the ecological safety of Bt proteins to Chrysopa pallens through tritrophic food chain, we investigated the effects of Cry1Ac, Cry1F and Cry2Ab insecticidal proteins that are widely transformed into several plant species on C. pallens using resistant target insects or insensitive non-target insects as preys. The results indicated that the three Bt proteins showed no significant adverse effects on the larvae (weight of the fourth instars and the larval duration), pupae (pupation rate, pupal weight and duration) and adults (eclosion rate, female and male weight and fecundity) of C. pallens through tritrophic food chain when using resistant Helicoverpa armigera (resistant to Cry1Ac or Cry2Ab) or resistant Ostrinia furnacalis (resistant to Cry1F) as the prey. Similarly, the three Bt proteins also had no obvious detrimental effects on the development and reproduction of C. pallens through tritrophic food chain when Bt-insensitive non-target insects Aploygus lucorum were used as the prey. Therefore, Cry1Ac, Cry1F and Cry2Ab protein, which have been widely transformed into current Bt cotton, Bt maize or Bt rice, showed no potential toxicity on the development and reproduction of C. pallens through tritrophic food chain.

Key words: Bt insecticidal protein, food chain, Chrysopa pallens, transgenic insect-resistant plants

中图分类号:

S476.2

赵曼, 汤金荣, 牛琳琳, 陈琳, 梁革梅. 不同Bt杀虫蛋白通过食物链对大草蛉的影响[J]. 中国生物防治学报, 2019, 35(4): 557-562.

ZHAO Man, TANG Jinrong, NIU Linlin, CHEN Lin, LIANG Gemei. Tritrophic Effects of Different Bt Insecticidal Proteins on Chrysopa pallens[J]. Chinese Journal Of Biological Control, 2019, 35(4): 557-562.

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不同Bt杀虫蛋白通过食物链对大草蛉的影响

Tritrophic Effects of Different Bt Insecticidal Proteins on Chrysopa pallens

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