灰茶尺蠖为害诱导茶树释放的互利素的鉴定

doi:10.16409/j.cnki.2095-039x.2019.05.023

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (1): 65-71.DOI: 10.16409/j.cnki.2095-039x.2019.05.023

灰茶尺蠖为害诱导茶树释放的互利素的鉴定

范培珍^1,2, 韩善捷¹, 韩宝瑜¹

1. 中国计量大学生命科学学院/浙江省生物计量与检验检疫技术重点实验室, 杭州 310018;
2. 六安职业技术学院, 六安 237158

收稿日期:2019-05-24 出版日期:2020-02-08 发布日期:2020-02-26
通讯作者: 韩宝瑜,博士,教授,E-mail:han-insect@263.net
作者简介:范培珍,硕士,副教授,E-mail:fanpeizhen@126.com
基金资助:
国家重点研发计划（2018YFC1604402）；荷兰Solidaridad基金会项目（SOL2010/714）

Identification of Synomones Emitted from Tea Plants Damaged by Ectropis grisescens

FAN Peizhen^1,2, HAN Shanjie¹, HAN Baoyu¹

1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2. Liu'an Vocational Technical College, Liu'an 237158, China

Received:2019-05-24 Online:2020-02-08 Published:2020-02-26

摘要/Abstract

摘要： 为鉴定灰茶尺蠖为害诱导茶树释放的互利素，遂以SDE法提取灰茶尺蠖为害茶梢和正常茶梢挥发物，经GC-MS检测，分别鉴定出31和25种组分，二者总含量相对于等剂量内标的含量分别是1863.8%和1124.6%。与正常茶梢相比，灰茶尺蠖为害茶梢挥发物中苯甲醛、反-2-癸烯醛、吲哚、水杨酸、反-2-己烯醛和己醛含量显著增加。将灰茶尺蠖为害茶梢挥发物的18种主要组分分别溶于液体石蜡制成10^-4g/mL味源，以Y形嗅觉仪检测茶尺蠖绒茧峰对味源的趋性；与液体石蜡相比，苯甲醛、水杨酸、反-2-己烯醛和己醛诱效显著，反-2癸烯醛、4-庚烯醛和吲哚的诱效明显，其他11组分无明显诱效。再将前7种组分配成10^-2g/mL引诱剂，分别载于橡皮头制成诱芯，附于芽绿粘板上，在茶园茶尺蠖绒茧蜂盛发期实施诱捕，携带前4种诱芯的芽绿粘板诱捕的蜂数明显多于后3种、且显著多于无诱芯的芽绿粘板，而且携带10^-2g/mL苯甲醛、水杨酸和反-2-己烯醛等量混合物诱芯的诱效最强。认为苯甲醛、水杨酸、反-2-己烯醛和己醛是灰茶尺蠖取食诱导的茶树互利素。

关键词: 灰茶尺蠖, 茶树互利素, 引诱, 茶尺蠖绒茧峰, 诱芯

Abstract: In order to identify the synomones released from tea plants damaged by Ectropis grisescens, the volatile compounds from E. grisescens-damaged tea shoots (EGDTS) and the intact tea shoots (ITS) were collected by the simultaneous distillation extraction method (SDE), and then detected by gas chromatography coupled of mass spectrometer. Thirty-one and twenty-five components were respectively identified, and the total contents of these volatile components from the two samples relative to internal standards of the same quality were respectively 1863.8% and 1124.6%. Compared with the volatiles from ITS, ample benzaldehyde, E-2-decenal and indole were detected in volatiles from EGDTS and contents of salicylic acid, E-2-hexenal and hexanal were significantly higher. The eighteen principal components identified from EGDTS were respectively dissolved in liquid paraffin to make a series of odor sources at 10^-4g/mL, and responses of the parasitic wasp Apanteles sp. to these odors were observed using Y-tube olfactometer. The results showed that benzaldehyde, compared with liquid paraffin, benzaldehyde, salicylic acid, E-2-hexenal and hexanal significantly attracted the wasps, E-2-decenal, 4-heptenal and indole showed obvious attraction, while the other eleven components showed no attraction. The seven components showing attractive activities were further prepared as attractants at 10^-2g/mL and then loaded onto rubber septa. A rubber spetum was singly attached on a bud green sticky board and used to trap the parasitic wasp at its peak occurrence. The wasp catches of the former 4 components were obviously more than those of the latter 3 components, and significantly more than those of the blank control. Especially, the blend of equal amount of benzaldehyde, salicylic acid and E-2-hexenal at 10^-2g/mL showed the strongest attraction. Therefore, benzaldehyde, salicylic acid, E-2-hexenal and hexanal are the synomones of tea plant induced by E. grisescens.

Key words: Ectropis grisescens, synomone of tea plant, attraction, Apanteles sp., lure

中图分类号:

S476

范培珍, 韩善捷, 韩宝瑜. 灰茶尺蠖为害诱导茶树释放的互利素的鉴定[J]. 中国生物防治学报, 2020, 36(1): 65-71.

FAN Peizhen, HAN Shanjie, HAN Baoyu. Identification of Synomones Emitted from Tea Plants Damaged by Ectropis grisescens[J]. Chinese Journal Of Biological Control, 2020, 36(1): 65-71.

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灰茶尺蠖为害诱导茶树释放的互利素的鉴定

Identification of Synomones Emitted from Tea Plants Damaged by Ectropis grisescens

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