广德市笄罩山毛竹根际绿僵菌物种多样性及生态位研究

doi:10.16409/j.cnki.2095-039x.2020.06.007

摘要/Abstract

摘要： 为研究毛竹Phyllostachys edulis根际绿僵菌Metarhizium spp.的组成及其生态位分布，本研究在安徽省广德市笄罩山按不同海拔、不同坡向分别设置了6处样地，于2018年秋、冬和2019年春、夏4个季节采集毛竹根系，分离根际绿僵菌。采用传统形态分类与分子鉴定相结合的方法鉴定分离菌株。结果显示:采集的样品中共分离出4种绿僵菌属真菌，分别是罗伯茨绿僵菌M. robertsii、平沙绿僵菌M. pingshaense、贵州绿僵菌M. guizhouense和一种待鉴定绿僵菌Metarhizium sp.，其中优势种为罗伯茨绿僵菌，广泛分布于海拔100 m处，而一种待鉴定绿僵菌则常见于海拔400 m处。罗伯茨绿僵菌数量在夏季达到峰值，其他3种绿僵菌的数量随月份的增加而递减。各绿僵菌生态位研究结果表明，4种绿僵菌的空间生态位宽度和空间生态位重叠，呈现明显的月变化；一种待鉴定绿僵菌的时间生态位宽度最大，贵州绿僵菌最小；一种待鉴定绿僵菌和贵州绿僵菌的时间生态位重叠值最高，贵州绿僵菌和罗伯茨绿僵菌时间生态位重叠值最低。

关键词: 毛竹, 根际, 绿僵菌, 生态位

Abstract: Rhizosphere Metarhizium strains were isolated from the roots of Phyllostachys edulis collected in autumn and winter of 2018 and spring and summer of 2019 from six sample plots set up at different altitudes and different slope directions in Jizhao Mountains, Guangde City, Anhui Province for analysis of the composition and niche distribution of Metarhizium in the rhizosphere of Phyllostachys edulis. The isolated strains were identified with the classic morphological classification and molecular methods. The results showed that four Metarhizium species including M. robertsii, M. pingshaense, M. guizhouense and an unknown species of Metarhizium were identified from the isolates. The dominant species is M. robertsii, distributing widely at 100 m above sea level, while Metarhizium sp. commonly distributes at 400 m above sea level. The population size of M. robertsii peaked in summer, while those of the other three species decreased with the progress of the month. The width and overlap of spatial niche of the four species of Metarhizium showed obvious changes with progress of month. The temporal niche width maximized in M. sp and minimized in M. guizhouense, the temporal niche of Metarhizium. sp and M. guizhouense overlapped the most, and the temporal niche of M. guizhouense and M. robertsii overlapped the least.

Key words: Phyllostachys edulis, rhizosphere, Metarhizium spp., niche

中图分类号:

S476.1

陈名君, 侯囡嵩, 林俨, 刘玉军, 黄勃. 广德市笄罩山毛竹根际绿僵菌物种多样性及生态位研究[J]. 中国生物防治学报, 2021, 37(1): 117-123.

CHEN Mingjun, HOU Nansong, LIN Yan, LIU Yujun, HUANG Bo. Species Diversity and Niche of Metarhizium spp. in the Rhizosphere of Phyllostachys edulis in Jizhao Mountains, Guangde City[J]. Chinese Journal of Biological Control, 2021, 37(1): 117-123.

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