三种农药对龟纹瓢虫细胞色素P450基因表达的影响

doi:10.16409/j.cnki.2095-039x.2021.11.020

摘要/Abstract

摘要： 为探究棉田常用杀菌剂、除草剂、植物生长调节剂对龟纹瓢虫的潜在影响，选取甲基硫菌灵、精喹禾灵、缩节胺对其成虫进行胁迫处理，分析其繁殖力与存活率变化及解毒酶的转录响应。经缩节胺、甲基硫菌灵和精喹禾灵处理后，龟纹瓢虫繁殖力和存活率均无显著性变化。从龟纹瓢虫基因组中鉴定出CYP307A1、CYP314A1、CYP345D2、CYP6BQ22、CYP9Z1和CYP9Z4共六个P450基因，蛋白序列结构分析发现其分别编码510、421、353、314、343和385个氨基酸，均包含P450结构域，且前三者属于CYP3家族、后二者属于CYP9家族，CYP6BQ22属于CYP6家族。利用实时荧光定量PCR技术检测了在不同农药处理下的上述基因表达量变化，结果表明，经甲基硫菌灵处理后，CYP307A1和CYP6BQ22表达量显著上调；经精喹禾灵处理后，CYP307A1、CYP345D2和CYP6BQ22表达量显著上调，CYP9Z1和CYP9Z4表达量显著下调；经缩节胺处理后，CYP307A1和CYP9Z1表达量显著上调。综上所述，虽然龟纹瓢虫经三种农药处理后生物学数据无显著性变化，但均可使部分P450基因表达上调，可能会促进龟纹瓢虫的解毒代谢效应。该研究为探究农药对龟纹瓢虫的潜在影响及其抗药性提供了重要证据。

关键词: 龟纹瓢虫, 细胞色素P450, 甲基硫菌灵, 精喹禾灵, 缩节胺

Abstract: The potential effects of commonly used fungicides, herbicides and plant growth regulators (thiophanate-methyl, quizalofop-p-ethyl, and mepiquat chloride) on Propylea japonica were measured by investigating the fertility, survival rate and the transcriptional response of detoxification enzymes. Interestingly, fecundity and survival rate of P. japonica showed no significant changes after the insects were treated with thiophanate-methyl, quizalofop-ethyl, or mepiquat chloride. Six P450 genes (CYP307A1, CYP314A1, CYP345D2, CYP6BQ22, CYP9Z1, CYP9Z4) were identified from the P. japonica genome. The protein sequence structure analysis revealed that the genes encode 510, 421, 353, 314, 343, 385 amino acids, respectively, and all contain Pfam P450 domain. The P450 genes CYP307A1, CYP314A1, CYP345D2 belong to the CYP3 family, the CYP9Z1 and CYP9Z4 belong to the CYP9 family, and CYP6BQ22 belongs to the CYP6 family. The real-time fluorescent quantitative PCR technology was used to detect the transcriptional responses of the genes in P. japonica to the treatment by the agricultural agents. The results showed that thiophanate-methyl treatment significantly up-regulated the expression levels of CYP307A1 and CYP6BQ22, quizalofop-p-ethyl stress significantly up-regulated the expression of CYP307A1, CYP345D2, and CYP6BQ22 while significantly down-regulated the expression of CYP9Z1 and CYP9Z4. The treatments with mepiquat chloride significantly up-regulated expression of CYP307A1 and CYP9Z1. Although no significant biological variations were observed in P. japonica treated with the three pesticides, several P450 genes expressions were up, which might contribute to the detoxification metabolic effect of P. japonica. This study provides important evidence for exploring the potential influences of agricultural agents on P. japonica.

Key words: Propylea japonica, cytochrome P450, thiophanate-methyl, quizalofop-p-ethyl, mepiquat chloride

中图分类号:

S476

常国凤, 薛汇, 皇甫宁博, 张开心, 李东阳, 王利沙, 姬继超, 马伟华. 三种农药对龟纹瓢虫细胞色素P450基因表达的影响[J]. 中国生物防治学报, 2022, 38(6): 1449-1458.

CHANG Guofeng, XUE Hui, HUANGFU Ningbo, ZHANG Kaixin, LI Dongyang, WANG Lisha, JI Jichao, MA Weihua. Influence of Three Pesticides on the Expression of Six P450 Genes in Propylea japonica[J]. Chinese Journal of Biological Control, 2022, 38(6): 1449-1458.

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