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中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (4): 1030-1036.DOI: 10.16409/j.cnki.2095-039x.2022.01.005

• 绿色防控方法与技术 • 上一篇    下一篇

亮腹釉小蜂产品的质量标准

陈玲玲1,2, 黄承志1,2, 毛润乾2, 修宝林2, 高晶2, 叶凤娴2, Mubasher Hussain2, 邱栋梁1, 申宝营1, 王朝红2   

  1. 1. 福建农林大学园艺学院, 福州 350002;
    2. 广东省科学院动物研究所/广东省动物保护与资源利用重点实验室/广东省野生动物保护与利用公共 实验室/广东省矿物油农药工程技术研究中心, 广州 510260
  • 收稿日期:2021-07-15 发布日期:2022-08-11
  • 通讯作者: 王朝红,博士后,E-mail:wangzh@giz.gd.cn
  • 作者简介:陈玲玲,硕士研究生,Email:1627236708@qq.com
  • 基金资助:
    广东省科学院科技发展专项(2022GDASZH-2022010106,2019GDASYL-0501006);广东省科技计划项目(2017B020202005,2016B090923009,2016B090923005);2022年广州市重点研发计划农业和社会发展科技专题项目-对口科技帮扶合作及城乡融合发展(47柑橘木虱专一性寄生蜂规模化生产技术与应用)

Quality Standard of Mass-Produced Tamarixia radiata

CHEN Lingling1,2, HUANG Chengzhi1,2, MAO Runqian2, XIU Baolin2, GAO Jing2, YE Fengxian2, Mubasher Hussain2, QIU Dongliang1, SHEN Baoying1, WANG Zhaohong2   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Guangdong Key Laboratory of Animal Conservation and Resource Utilization/Guangdong Public Laboratory of Wild Animal Conservation and Utilization/Guangdong Engineering Research Center for Mineral Oil Pesticides/Institute of Zoology, Guangdong Academy of Science, Guangzhou 510260, China
  • Received:2021-07-15 Published:2022-08-11

摘要: 本文对工厂化生产的亮腹釉小蜂Tamarixia radiata (Waterston)成虫产品进行了一系列检测,检测物种形态及分子特征、外观(体色暗淡率、残肢残翅率)、产品数量、雌蜂率、寄生率及不同温度下(15℃、25℃及30℃)亮腹釉小蜂贮存死亡率共7个质量指标,并根据检测结果提出了亮腹釉小蜂产品的质量标准。结果表明,亮腹釉小蜂规模化生产的产品应符合以下标准:形态特征吻合,分子序列(COI、ITS-1和ITS-2)相似度 ≥ 97%,体色暗淡率 ≤ 2%、残肢残翅率 ≤ 1%、实际数量占标识数量的比率 ≥ 95%、雌蜂率 ≥ 70%、不同温度下贮存死亡率均 ≤ 5%、寄生率 ≥ 90%。本标准各项指标的准确性和专属性强、灵敏度高,具有较高可行性,可用于亮腹釉小蜂质量控制和质量管理。

关键词: 亮腹釉小蜂, 质量标准, 工厂化生产, 产品

Abstract: A series of tests of the factory-produced Tamarixia radiata (Waterston) adults were carried out in this study,including the morphology and molecular characteristics,appearance (body color,residual limb and residual wing rate),product quantity,female rate,parasitic rates,storage mortalities at 15℃,25℃ and 30℃.According to the results,the quality standard of the mass-produced T.radiata is proposed.Morphologically,the wasps should be consistent.Molecular sequences (COI,ITS-1 and ITS-2) should be similar at more than 97%.Wasps of dim body color should be less than 2%.Deformity rate is less than 1%.The ratio of actual quantity to labeled quantity is more than 95%.Female wasp rate is more than 70%.Mortalities at 15℃,25℃ and 30℃ are less than 5%.Parasitisam rate is more than 90%.The parameters of this standard are specific,accurate,sensitive and easy to use in the production of T.radiata for the quality control and management.

Key words: Tamarixia radiata, quality standard, factory production, products

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