球孢白僵菌内生定殖豇豆对美洲斑潜蝇种群的影响

doi:10.16409/j.cnki.2095-039x.2024.07.018

摘要/Abstract

摘要： 为明确球孢白僵菌Beauveria bassiana对豇豆Vigna unguiculata上美洲斑潜蝇Liriomyza sativae种群的防控效果，本文在实验室内分别以球孢白僵菌内生定殖的豇豆和健康豇豆植株为寄主植物，构建美洲斑潜蝇室内实验种群的年龄-阶段特异生命表，评价球孢白僵菌内生定殖豇豆对美洲斑潜蝇生长发育、繁殖和种群参数的影响。此外，田间调查比较球孢白僵菌定殖的豇豆与对照植株上美洲斑潜蝇的种群发展动态。室内试验结果表明：在球孢白僵菌定殖组与对照组之间，美洲斑潜蝇的发育历期、存活率和繁殖率均存在显著差异。其中，球孢白僵菌定殖组美洲斑潜蝇卵期（3.00 d）比对照组（2.92 d）显著延长；尽管两组间的全幼虫期和蛹期无显著差异，但球孢白僵菌定殖组美洲斑潜蝇的蛹重（0.43 g/千粒）明显低于对照（0.49 g/千粒）。球孢白僵菌定殖组成虫前期存活到成虫期的概率（29.88%）显著低于对照（83.33%）。球孢白僵菌定殖组美洲斑潜蝇雌虫寿命（3.92 d）、雄虫寿命（3.69 d）和产卵总量（71.96）均显著低于对照组（雌虫： 7.48 d；雄虫： 6.23 d；总产卵量： 140.92）；球孢白僵菌定殖组美洲斑潜蝇种群的内禀增长率（r）（0.1385/d）、周限增长率（λ）（1.1486/d）和净增殖率（R₀）（10.3222）分别低于对照组的0.2448/d、1.2774/d和62.6333。田间试验结果表明：调查期内，处理组的斑潜蝇种群数量始终低于对照组。综上所述，与对照相比，取食球孢白僵菌内生定殖的豇豆植株的美洲斑潜蝇种群受到了明显抑制作用。将球孢白僵菌作为豇豆的内共生真菌防治美洲斑潜蝇具有可行性。

关键词: 美洲斑潜蝇, 球孢白僵菌, 豇豆, 内生定殖, 种群

Abstract: This study aimed to elucidate the control of Liriomyza sativae by Beauveria bassiana on cowpea Vigna unguiculata. With B. bassiana-colonized cowpea and non-colonized cowpea (the control) as the host plants, the growth, development, reproduction, and population parameters of L. sativae were measured. In addition, the population dynamics of L. sativae was compared between B. bassian-colonized cowpea and control cowpea in a field test. The results of laboratory experiments showed significant differences in the development period, survival rate, and reproductive rate of L. sativae. Specifically, the egg period of L. sativae on B. bassian-colonized cowpea was significantly prolonged, although there was no significant difference in the total larval and pupal periods between the two groups. The pupal weight of L. sativae in B. bassian-colonized group (0.43 g/1000 pupae) was significantly lower than that in the control (0.49 g/1000 pupae). The probability of survival from the pre-adult stage to the adult stage in B. bassian-colonized group (29.88%) was significantly lower than that in the control (83.33%). The lifespans of female (3.92 days) and male (3.69 days) adults, as well as the total egg production (71.96), in the treatment group were significantly lower than those in the control group (female: 7.48 days; male: 6.23 days; total egg production: 140.92). The intrinsic growth rate (r) (0.1385/day), finite growth rate (λ) (1.1486/day), and net reproductive rate (R₀) (10.3222) in the treatment group were lower than those in the control group (r: 0.2448/day; λ: 1.2774/day; R₀: 62.6333). Field experiment results showed that the population size of L. sativae in B. bassian-colonized group was consistently lower than that in the control group throughout the investigation period. In summary, compared with the control, the population of L. sativae feeding on cowpea plants with endophytic colonization of B. bassiana is significantly suppressed, indicating the feasibility of using B. bassiana as an endophytic fungus for controlling L. sativae on cowpea.

Key words: Liriomyza sativae, Beauveria bassiana, Vigna unguiculata, endophytic colonization, population

中图分类号:

S476.1

钟绍忆, 梁兴慧, 王登杰, 李桂英, 张治科, 王海鸿, 雷仲仁, 吴圣勇, 高玉林. 球孢白僵菌内生定殖豇豆对美洲斑潜蝇种群的影响[J]. 中国生物防治学报, 2025, 41(3): 691-701.

ZHONG Shaoyi, LIANG Xinghui, WANG Dengjie, LI Guiying, ZHANG Zhike, WANG Haihong, LEI Zhongren, WU Shengyong, GAO Yulin. The Effect of Endophytic Colonization of Cowpeas by Beauveria bassiana on Liriomyza sativae[J]. Chinese Journal of Biological Control, 2025, 41(3): 691-701.

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