CO2浓度增加条件下球孢白僵菌定殖对玉米促生和抗性作用的转录组学分析

doi:10.16409/j.cnki.2095-039x.2024.11.008

摘要/Abstract

摘要： 为解析CO₂浓度增加条件下球孢白僵菌定殖对植物作用的潜在分子机制，本研究以玉米B73品种为试验材料，采用盆栽控制试验，在开顶式气室内研究CO₂浓度增加条件下球孢白僵菌定殖对玉米植株生长和抗性的影响，对玉米叶片RNA进行转录组测序，并进行生物信息学分析，以探讨CO₂浓度增加条件下球孢白僵菌定殖对植物作用的相关机制。通过转录组测序和荧光定量PCR验证，明确了正常CO₂浓度（Control），CO₂浓度增加（eCO₂）和球孢白僵菌定殖（Bb）对玉米体内促生和抗性相关基因表达之间的显著影响。Bb与eCO₂+Bb，Control与Bb，Control与eCO₂和eCO₂与eCO₂+Bb各组差异表达基因数目分别为907、803、589和669个，其中上调基因数目分别为407、429、360和238个，下调基因数目分别为500、374、229和431个。不同处理组样本两两比较后对比组之间共表达基因数目有16个。差异表达基因中与植物生长和抗性相关的基因主要富集在代谢途径、次生代谢产物的生物合成、糖胺聚糖降解、类固醇生物合成、二萜生物合成和苯并恶嗪类生物合成等通路中，差异基因qRT-PCR验证结果与转录组分析结论一致。CO₂浓度增加和球孢白僵菌定殖在一定程度上对玉米生长和抗性相关的功能基因和途径具有正向调控作用，进而对玉米植株的生长发育产生积极影响。

关键词: 气候变化, 虫生真菌, 内生定殖, 促生作用, 转录组

Abstract: The objective of this study was to clarify the underlying molecular mechanism of Beauveria bassiana colonization on plant growth and resistance under elevated CO₂. The study, employing maize B73 cultivar as the plant material and open top chambers for enrichment of CO₂, measured the growth and stress resistance of maize plants with B. bassiana colonization and explored the underlying mechanism through transcriptome sequencing and bioinformatics analysis. Transcriptome sequencing and fluorescence quantitative PCR confirmed that, when compared with normal atmospheric CO₂ concentration (Control), the increase of CO₂ concentration (eCO₂) and the colonization of B. bassiana (Bb) had significant effects on the expression of genes related to resistance and growth promotion in maize. The comparisons of Bb vs eCO₂+Bb, Control vs Bb, Control vs eCO₂, and eCO₂ vs eCO₂+Bb revealed 907, 803, 589, and 669 differentially expressed genes (DEGs), respectively, which included 407, 429, 360, and 238 genes with up-regulated expression and 500, 374, 229, and 431 genes with down-regulated expression, respectively. Among these DEGs, 16 were common in the pairwise comparisons of samples from different treatment groups. The DEGs related to plant growth and resistance were mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, glycosaminoglycan degradation, steroid biosynthesis, the pathways of diterpenoid biosynthesis and benzoxazinoid biosynthesis. The qRT-PCR verified the transcriptome analysis results of DEGs. The increase of CO₂ concentration and the colonization of B. bassiana have a positive regulatory effect on the functional genes and pathways related to maize growth and resistance to a certain extent, and then have a positive impact on the growth and development of maize plants.

Key words: climate change, entomopathogenic fungi, endophytic, growth promotion, transcriptome

中图分类号:

S476.1

文欢, 张正坤, 路杨, 隋丽, 李启云. CO₂浓度增加条件下球孢白僵菌定殖对玉米促生和抗性作用的转录组学分析[J]. 中国生物防治学报, 2025, 41(5): 1063-1077.

WEN Huan, ZHANG Zhengkun, LU Yang, SUI Li, LI Qiyun. Transcriptomic Analysis of the Effects of Beauveria bassiana Colonization on Maize Growth and Resistance under Elevated CO₂[J]. Chinese Journal of Biological Control, 2025, 41(5): 1063-1077.

参考文献

[1] Qin X, Zhao X, Huang S, et al. Pest management via endophytic colonization of tobacco seedlings by the insect fungal pathogen Beauveria bassiana[J]. Pest Management Science, 2021, 77: 2007-2018.
[2] 武玉环, 彭焕, 葛逢勇, 等. 5株生防真菌对孢囊线虫的杀线活性测定[J]. 生物技术通报, 2022, 38(11): 220-226.
[3] Rivas-Franco F, Hampton G J, Morán-Diez E M, et al. Effect of coating maize seed with entomopathogenic fungi on plant growth and resistance against Fusarium graminearum and Costelytra giveni[J]. Biocontrol Science and Technology, 2019, 29(9): 877-900.
[4] 隋丽, 路杨, 姜媛媛, 等. 内生性虫生真菌在生物防治中的研究现状与展望[J]. 玉米科学, 2021, 29(6): 169-174, 183.
[5] Russo M L, Jaber L R, Scorsetti A C, et al. Effect of entomopatho-genic fungi introduced as corn endophytes on the development, reproduction, and food preference of the invasive fall armyworm Spodoptera frugiperda[J]. Journal of Pest Science, 2020, 150: 104347.
[6] 谢敏, 路杨, 张云月, 等. 球孢白僵菌-玉米共生体对亚洲玉米螟个体发育的影响及其机理[J]. 应用昆虫学报, 2023, 60(6): 1851-1859.
[7] 隋丽, 路杨, 谢敏, 等. 亚洲玉米螟对球孢白僵菌分生孢子和芽生孢子-玉米共生体的取食选择和嗅觉反应[J]. 昆虫学报, 2023, 66(11): 1482-1489.
[8] Corneille J F, Thomas B, Frédéric F. Direct and indirect effect via endophytism of entomopathogenic fungi on the fitness of Myzuspersicae and its ability to spread PLRV on tobacco[J]. Insects, 2021, 12(2): 89.
[9] Jaber L R, Salem N M. Endophytic colonisation of squash by the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales) for managing Zucchini yellow mosaic virus in cucurbits[J]. Bio-control Science and Technology, 2014, 24(10): 1096-1109.
[10] Jaber L R. Grapevine leaf tissue colonization by the fungal entomopathogen Beauveria bassiana s.l. and its effect against downy mildew[J]. BioControl, 2015, 60(1): 103-112.
[11] 隋丽, 路杨, 迟瑞凯, 等. 玉米大斑病胁迫下球孢白僵菌对玉米植株的影响及定殖规律[J]. 中国生物防治学报, 2023, 39(4): 804-812.
[12] 李彦生, 金剑, 刘晓冰. 作物对大气CO₂浓度升高生理响应研究进展[J]. 作物学报, 2020, 46(12): 1819-1830.
[13] Wei Z H, Du T S, Li X N, et al. Interactive effects of elevated CO₂ and N fertilization on yield and quality of tomato grown under reduced irrigation regimes[J]. Frontiers in Plant Science, 2018, 9: 328.
[14] 宗毓铮, 张函青, 李萍, 等. 大气CO₂与温度升高对北方冬小麦旗叶光合特性、碳氮代谢及产量的影响[J]. 中国农业科学, 2021, 54(23): 4984-4995.
[15] 赵光影, 刘景双, 王洋. CO₂浓度升高与氮添加对三江平原湿地小叶章生长的影响[J]. 应用生态学报, 2011, 22(6): 1653-1658.
[16] Li H, Wang Z, Li S, et al. Multigenerational elevated atmospheric CO₂ concentration induced changes of wheat grain quality via altering nitrogen reallocation and starch catabolism[J]. Environmental and Experimental Botany, 2023, 205: 105-127.
[17] 蒋跃林, 姚玉刚, 张庆国, 等. 大气二氧化碳浓度升高条件下大豆光合色素含量的变化[J]. 作物研究, 2006, 20(2): 144-146.
[18] Sui L, Zhu H, Wang D, et al. Tripartite interactions of an endophytic entomopathogenic fungus, Asian corn borer, and host maize under elevated carbon dioxide[J]. Pest Management Science, 2024, 80(9): 4575-4584.
[19] Lee S H, Woo S Y, Je S M. Effects of elevated CO₂ and water stress on physiological responses of Perilla frutescens var. japonica HARA[J]. Plant Growth Regulation, 2015, 75(2): 427-434.
[20] 张春兰, 秦孜娟, 王桂芝, 等. 转录组与RNA-Sep技术[J]. 生物技术通报, 2012(12): 51-56.
[21] Pleijel H, Sk?rby L, Wallin G, et al. Yield and grain quality of spring wheat (Triticum aestivum L. cv. Drabant) exposed to different concentrations of ozone in open-top chambers[J]. Enironmental Pollution, 1991, 69(2-3): 151-168.
[22] Rangaswamy T, Sridhara S, Ramesh N, et al. Assessing the impact of higher levels of CO₂ and temperature and their interactions on tomato (Solanum lycopersicum L.)[J]. Plants, 2021, 10(2): 256.
[23] Teri K, Kristiina R, Kaisa R, et al. Fluxes of N₂O, CH₄ and CO₂ in a meadow ecosystem exposed to elevated ozone and carbon dioxide for three years[J]. Enironmental Pollution, 2007, 145(3): 818-828.
[24] De Costa W A, Weerakoon W M, Herath H M, et al. Physiology of yield determination of rice under elevated carbon dioxide at high temperatures in a subhumid tropical climate[J]. Field Crops Research, 2006, 96(2-3): 336-347.
[25] Sui L, Zhu H, Xu W, et al. Elevated air temperature shifts the interactions between plants and endophytic fungal entomopathogens in an agroecosystem[J]. Fungal Ecology, 2020, 47: 100940.
[26] 周如月, 林嘉龙, 李烨凡, 等. 灵芝β-葡萄糖苷酶基因的克隆与功能分析[J]. 菌物学报, 2022, 41(12): 1971-1979.
[27] Erb M, Kliebenstein D. Plant secondary metabolites as defenses, regulators, and primary metabolites: the blurred functional trichotomy[J]. Plant physiology, 2020, 184(1): 39-52.
[28] Wang Y, Wang Q, Zhao Y, et al. Systematic analysis of maize class III peroxidase gene family reveals a conserved subfamily involved in abiotic stress response[J]. Gene, 2015, 566(1): 95-108.
[29] Simonetti E, Veronico P, Melillo M, et al. Analysis of class III peroxidase genes expressed in roots of resistant and susceptible wheat lines infected by Heterodera avenae[J]. Molecular Plant-Microbe Interactions, 2009, 22(9): 1081.
[30] 黄先忠, 蒋才富, 廖立力, 等. 赤霉素作用机理的分子基础与调控模式研究进展[J]. 植物学通报, 2006(5): 499-510.
[31] 叶元土, 吴萍, 蔡春芳, 等. 基于类固醇合成途径的生物进化及其产物的饲料资源利用[J]. 饲料工业, 2023, 44(6): 1-10.
[32] Hedden P, Thomas S G. Gibberellin biosynthesis and its regulation [J]. The Biochemical journal, 2012, 444(1): 11-25.
[33] Celedon J M, Bohlmann J. Oleoresin defenses in conifers: chemical diversity, terpene synthases and limitations of oleoresin defense under climate change[J]. New Phytologist, 2019, 224(4): 1444-1463.
[34] Otsuka H, Hirai Y, Nagao T, et al. Anti-inflammatory activity of benzoxazinoids from roots of Coix lachryma-jobi var. ma-yuen[J]. Journal of Natural Products, 2004, 51(1): 74-79.
[35] 隋丽, 万婷玉, 路杨, 等. 内生真菌对植物促生、抗逆作用研究进展[J]. 中国生物防治学报, 2021, 37(6): 1325-1331.

CO₂浓度增加条件下球孢白僵菌定殖对玉米促生和抗性作用的转录组学分析

Transcriptomic Analysis of the Effects of Beauveria bassiana Colonization on Maize Growth and Resistance under Elevated CO₂

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

联系我们

[1]	黄震, 刘思, 农向群, 高琼华. 防控红火蚁球孢白僵菌饵剂的载体饵料与引诱剂筛选[J]. 中国生物防治学报, 2025, 41(3): 680-690.
[2]	钟绍忆, 梁兴慧, 王登杰, 李桂英, 张治科, 王海鸿, 雷仲仁, 吴圣勇, 高玉林. 球孢白僵菌内生定殖豇豆对美洲斑潜蝇种群的影响[J]. 中国生物防治学报, 2025, 41(3): 691-701.
[3]	张维佳, 杨洪佳, 王艺晓, 威力斯, 吕佳炜, 单志勐, 张鑫鑫, 樊东. 黏虫乙酰胆碱酯酶基因MsAChE克隆与生物学功能[J]. 中国生物防治学报, 2025, 41(2): 309-320.
[4]	漆婷, 汪辛宇, 黄世会, 胡冯彬, 徐婷婷, 梁才康, 吴丽娟, 牛熙, 冉雪琴, 王嘉福. 长枝木霉菌与黄曲霉菌互作期间基因表达谱分析[J]. 中国生物防治学报, 2025, 41(2): 321-334.
[5]	朱文亭, 张梦宁, 赵培怡, 王子明, 施艳, 孙炳剑, 陈琳琳, 李洪连. 小麦茎基腐病拮抗菌FCR-Y1的分离鉴定及其防病促生效果[J]. 中国生物防治学报, 2025, 41(2): 373-383.
[6]	王恩东, 明蒙, 刘正玲, 谢永辉, 张博, 徐学农. 巴氏新小绥螨和剑毛帕厉螨与球孢白僵菌田间联合应用防治烟草西花蓟马[J]. 中国生物防治学报, 2024, 40(6): 1243-1249.
[7]	黄鹏, 张杰, 姚锦爱, 余德亿, 侯翔宇. 金龟子绿僵菌和蜡蚧轮枝菌共培养对石蒜绵粉蚧的室内侵染活性和防治效果[J]. 中国生物防治学报, 2024, 40(6): 1267-1274.
[8]	张萌, 陈森, 唐登国, 黄艳, 龙治坚, 王博雅, 胡尚连, 曹颖. 比较转录组分析揭示贝莱斯芽胞杆菌激活JA和SA途径增强花魔芋对软腐病抗性[J]. 中国生物防治学报, 2024, 40(6): 1375-1385.
[9]	赵誉霞, 陈丽华, 贾小琦, 邵朝阳, 舒金平, 张亚波. 九龙山自然保护区四种生境土壤的虫生真菌多样性[J]. 中国生物防治学报, 2024, 40(5): 1135-1148.
[10]	区翠仪, 方康, 张珂, 胡琼波, 翁群芳. 罗伯茨绿僵菌与苦参碱复配防治瓜蚜[J]. 中国生物防治学报, 2024, 40(4): 820-827.
[11]	王芳, 宁丽平, 邓稳桥, 秦菁菁, 许秀美, 孙正祥, 程毅. 贝莱斯芽胞杆菌C1B1生防活性及促生作用研究[J]. 中国生物防治学报, 2024, 40(4): 948-957.
[12]	胡亚杰, 范啟铃, 李群岭, 周效峰, 丁婷, 汪章勋, 吴峰. 枯草芽胞杆菌GXHZ16抑制烟草疫霉病菌的表达谱分析[J]. 中国生物防治学报, 2024, 40(3): 608-619.
[13]	田艺帆, 张正坤, 隋丽, 赵宇, 路杨, 孟钊, 石旺鹏, 李启云. 斜纹夜蛾高致病力生防真菌的筛选与鉴定[J]. 中国生物防治学报, 2024, 40(2): 282-290.
[14]	胡育硕, 张春雨, 孟祥昕, 李朔涵, 张津博, 樊东. 黏虫保幼激素结合蛋白基因*MsJHBP3*生物学功能及对黏虫生长发育的影响 [J]. 中国生物防治学报, 2024, 40(1): 80-90.
[15]	段天坤, 王燕, 袁佳琪, 苏雅馨, 史璐欣, 王琳, 苏健, 王美琴, 王春伟. 异辛醇对灰葡萄孢的抑菌作用及转录组分析[J]. 中国生物防治学报, 2023, 39(6): 1434-1445.