水稻香豆素对褐飞虱的抗性作用

doi:10.16409/j.cnki.2095-039x.2025.09.019

摘要/Abstract

摘要： 次生代谢物在植物防御病虫害过程中发挥重要作用。本研究基于前期水稻代谢组分析结果，采用HPLC技术测定了目标成分香豆素的含量，发现褐飞虱取食的抗性水稻R1叶鞘中香豆素含量相较感性水稻S9增长2.88倍。将香豆素添加到人工取食液饲喂褐飞虱，结果表明，0.1 mg/mL和0.5 mg/mL香豆素处理褐飞虱若虫7 d后，存活率相较于对照组分别下降38.89%和80%。香豆素胁迫下雌成虫体重减少；雌虫平均产卵量减少89粒且孵化率下降16.29%；内共生真菌的数量在5 d后相较于对照组下降49.73%，其中Ascomycetes和Arsenophonus等内共生真菌在褐飞虱多个组织中有不同程度的降低。通过分子对接技术分析了香豆素与褐飞虱候选靶蛋白碳酸酐酶的互作特点，初步揭示了香豆素对褐飞虱的作用机制。本研究明确了水稻香豆素对褐飞虱及其共生真菌的抗性作用，为水稻抗虫机制研究及害虫绿色防控提供了新的线索。

关键词: 水稻, 香豆素, 褐飞虱, 共生菌

Abstract: Secondary metabolites play a crucial role in plant defense against pests and diseases. Based on previous metabolomic analyses of rice, this study employed HPLC to determine the content of the target compound coumarin. The results revealed that, when the rice plants were infested by the brown planthopper(BPH), the coumarin content in the leaf sheaths of the resistant rice line R1 was increased by 2.88 folds compared to the susceptible line S9. When coumarin was added to an artificial diet at 0.1 mg/mL and 0.5 mg/mL and fed to BPH, the survival rates of nymphs decreased by 38.89% and 80% at 7 days after treatment, respectively, compared to the control. Coumarin stress also led to a reduction in adult female body weight, an average decrease of 89 eggs in oviposition, and a 16.29% reduction in egg hatchability. The abundance of endosymbiotic fungi was decreased by 49.73% at 5 days after treatment, with significant reductions observed in fungi such as Ascomycetes and Arsenophonus in various BPH tissues. Molecular docking analysis revealed the interaction characteristics between coumarin and the candidate target protein carbonic anhydrase in BPH, providing preliminary insights into the mechanism of coumarin's effects on BPH. This study elucidates the resistance role of rice-derived coumarin against BPH and its endosymbiotic fungi, offering new clues for understanding rice insect resistance mechanisms and for the development of environmentally friendly pest management strategies.

Key words: rice, coumarin, brown planthopper, symbiotic bacteria

中图分类号:

S476

姚欣璇, 周雯润, 陈桐桐, 俞晓平, 郝培应. 水稻香豆素对褐飞虱的抗性作用[J]. 中国生物防治学报, 2026, 42(3): 569-581.

YAO Xinxuan, ZHOU Wenrun, CHEN Tongtong, YU Xiaoping, HAO Peiying. Resistance to the Brown Planthopper Afforded by the Secondary Metabolite Coumarin in Rice[J]. Chinese Journal of Biological Control, 2026, 42(3): 569-581.

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