蠋蝽非典型气味受体基因的克隆及组织表达谱分析

doi:10.16409/j.cnki.2095-039x.2025.11.002

摘要/Abstract

摘要： 为明确捕食性天敌昆虫蠋蝽Arma chinensis的非典型气味受体共受体（Odorant receptor co-receptor，Orco）基因的分子特性及其不同组织表达谱，利用RT-PCR技术克隆蠋蝽的Orco基因，对Orco蛋白进行结构预测和系统发育分析，并采用qPCR技术测定Orco基因在雌雄成虫不同组织中的表达量。结果表明，克隆获得蠋蝽Orco基因并命名为AchiOrco（GenBank登录号为PP965794）。该基因开放阅读框全长1425 bp，编码474个氨基酸。AchiOrco蛋白分子量为53.41 kD，等电点为6.10，无信号肽，为非分泌性蛋白；具有7个跨膜结构域，是疏水性膜蛋白；蛋白质空间结构主要由α螺旋和无规则卷曲缠绕折叠组成。系统发育分析表明蠋蝽与茶翅蝽Halyomorpha halys亲缘关系最近。qPCR结果显示，AchiOrco基因在蠋蝽成虫触角中的表达量显著高于其他组织，且在雌成虫触角中的表达量最高，为雄成虫触角中表达量的1.33倍；AchiOrco基因在雌成虫腹部中的表达量显著高于雄成虫腹部的表达量，在喙、头、胸、足和翅中有少量表达。本文克隆获得蠋蝽Orco基因序列，分析其蛋白结构具有昆虫非典型气味受体的结构特征。qPCR结果表明，蠋蝽Orco基因主要在触角中表达，触角是昆虫主要的嗅觉器官，推测Orco在蠋蝽嗅觉识别和信号传导中发挥重要作用。

关键词: 蠋蝽, 非典型气味受体, 基因克隆, 跨膜域, 组织表达谱

Abstract: To determine the molecular characteristics and tissue expression profiles of the odorant receptor co-receptor (Orco) gene in Arma chinensis, a predatory species successfully used for the biocontrol of lepidopteran and coleopteran pests. The Orco gene was cloned using reverse transcription-PCR (RT-PCR) techniques. Structural and phylogenetic analysis was performed on the Orco protein, and the expression of the Orco gene in different adult tissues was determined using qPCR． The cloned Orco gene of A. chinensis was named AchiOrco (GenBank acc. no. PP965794). The open reading frame of this gene is 1425 bp and encodes 474 amino acids. The secondary structure of AchiOrco protein mainly consists of α helices and irregular coiled-coil folds; The AchiOrco protein is an non-secretory protein without a signal peptide, with a molecular weight and isoelectric point of 53.41 kD and 6.10, respectively. It has seven transmembrane domains and was thus identified as a hydrophobic transmembrane protein. Phylogenetic analysis suggests that AchiOrco is closely related to the Orco gene of Halyomorpha halys, a hemipteran pest. qPCR results indicate that AchiOrco is mainly expressed in the antennae of adult A. chinensis, with relatively low expression in the proboscis, head, thorax, abdomen, legs and wings. Expression of AchiOrco in adult female antennae was 1.33-fold higher than in adult male antennae. Expression in the abdomen was also significantly higher in females than males. Analysis of the AchiOrco protein structure indicates that it is structurally atypical compared to other insect odorant receptors. The qPCR results indicate that the AchiOrco gene is mainly expressed in the adult antennae, which is the main insect olfactory organ. It is possible, therefore, that the Orco gene plays an important role in olfactory recognition and signaling in insects.

Key words: Arma chinensis, odorant receptor co-receptor, gene cloning, transmembrane domain, tissue expression profile

中图分类号:

S476.2

王娟, 张福玉, 虎都, 朱文雅, 张礼生, 郭义, 张烨. 蠋蝽非典型气味受体基因的克隆及组织表达谱分析[J]. 中国生物防治学报, 2025, 41(5): 1054-1062.

WANG Juan, ZHANG Fu-Yu, HU Du, ZHU Wen-Ya, ZHANG Li-Sheng, GUO Yi, ZHANG Ye. Cloning and tissue expression profiling of the odorant receptor co-receptor gene in Arma chinensis (Hemiptera: Pentatomidae)[J]. Chinese Journal of Biological Control, 2025, 41(5): 1054-1062.

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