Abstract: Methanol extract from cell-free supernatants of Bacillus subtilis B006 suppressed hyphal growth of Fusarium oxysporunm f. sp. cucumerinum more greatly than that of Phytophthora capsici, while suppressed spore germination of F. oxysporunm f. sp. cucumerimerinum less than that of zoospores of P. capsici. The inhibition was not changed when the extract was heated at 60, 90, or 120℃ for 10 minutes. TLC analysis of the HCL hydrolysis solution of the extract gave rise to orange spots, indicating that the extract belongs to cyclopeptide. HPLC-ESI-MS analysis showed that the extract contained surfactin and fengycin with m/z at 995, 1009, 1023, 1037, 1051 and 1436, 1450, 1478, 1492. When cucumber and pepper were planted in sterile nursery substrate inoculated with B006 at 10⁶ cfu·g^-1, surfactin and fengycin could also be detected with HPLC-ESI-MS from SPE-extracted lipopeptides of roots of the plants at 4 to 6 leaf stage, while not from that of uninoculated plant roots. This research indicated that B. subtilis B006 produced lipopeptides, an important group of antibiotics, in plant rhizosphere, suggesting its possible role in biological control of soilborne plant disease in vivo.