生防菌Pc01-6对丹参根腐病的生防效果及基因组学分析

doi:10.16409/j.cnki.2095-039x.2025.02.015

摘要/Abstract

摘要： 为发掘丹参根腐病的优良生防资源，本研究通过平板对峙法从丹参根际土壤中分离筛选出1株高效拮抗菌株Pc01-6。该菌株对丹参根腐病原菌的抑制率达到63.67%～68.40%，并对其他10种植物病原真菌表现出广谱拮抗活性。经形态学、生理生化特性分析及16S rDNA和gyrB序列鉴定，确定菌株Pc01-6为绿针假单胞菌桔黄亚种Pseudomonas chlororaphis subsp.aurantiaca。盆栽试验结果显示，菌株Pc01-6对丹参根腐病的防治效果为53.30%～65.22%，同时显著促进丹参生长，使茎叶鲜重和根鲜重分别增加35.10%和72.34%。大田试验进一步证实，该菌株对丹参根腐病的田间防效达50.91%。全基因组测序分析表明，菌株Pc01-6基因组大小为6 835 850 bp，GC含量为62.87%，共编码6159个基因；基因功能预测显示，其基因组中含有环脂肽类化合物、硝吡咯菌素等多种具有抑菌活性的次生代谢产物生物合成相关基因簇。综上，菌株Pc01-6对丹参根腐病具有显著生防效果，并展现出良好的应用潜力。

关键词: 丹参根腐病, 拮抗菌株, 绿针假单胞菌桔黄亚种, 全基因组测序, 次生代谢产物生物合成基因簇

Abstract: In this study, an effective antagonistic isolate, Pc01-6, was isolated and screened from the rhizosphere soil of Salvia miltiorrhiza using the plate confrontation method to explore superior bioncontrol resources against S. miltiorrhiza root rot. The isolate exhibited an inhibition rate of 63.67% to 68.40% against the root rot pathogen, and demonstrated broad-spectrum antagonistic activity against 10 other plant pathogenic fungi. Based on morphological, physiological and biochemical characteristics, as well as 16S rDNA and gyrB sequence analysis, the isolate Pc01-6 was identified as Pseudomonas chlororaphis subsp. Aurantiaca. Pot experiments revealed that the isolate provided a control efficacy of 53.30% to 65.22% against S. miltiorrhiza root rot, while significantly promoting plant growth, with increases of 35.10% and 72.34% in fresh stem and leaf weight and root weights, respectively. Field trials further confirmed its efficiency, showing a control rate of 50.91% against root rot. Whole-genome sequencing analysis indicated that the genome of Pc01-6 is 6,835,850 bp in size, with a GC content of 62.87%, and encodes 6,159 genes. Function gene prediction revealed the presence of gene clusters associated with the biosynthesis of secondary metabolites with antibacterial activity, such as cyclolipopeptides and pyrrolnitrin. In summary, isolate Pc01-6 demonstrates significant biocontrol effects against S. miltiorrhiza root rot and holds promise for practical applications.

Key words: Salvia miltiorrhiza root rot, antagonistic isolate, Pseudomonas chlororaphis subsp. Aurantiaca, whole genome sequencing, secondary metabolite biosynthesis gene cluster

中图分类号:

S476

王飞, 李雪梦, 杨瑾, 高素霞, 郭治辰, 秦艳红, 文艺, 鲁书豪, 赵莹, 宋露洋, 杨健, 万修福, 李绍建. 生防菌Pc01-6对丹参根腐病的生防效果及基因组学分析[J]. 中国生物防治学报, 2025, 41(3): 542-553.

WANG Fei, LI Xuemeng, YANG Jin, GAO Suxia, GUO Zhichen, QIN Yanhong, WEN Yi, LU Shuhao, ZHAO Ying, SONG Luyang, YANG Jian, WAN Xiufu, LI Shaojian. Biocontrol Effects of Antagonistic Isolate Pc01-6 on Salvia miltiorrhiza Root Rot and Its Genomic Analysis[J]. Chinese Journal of Biological Control, 2025, 41(3): 542-553.

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