一株绿针假单胞菌桔黄亚种在防治番茄匍柄霉叶斑病中的应用

doi:10.16409/j.cnki.2095-039x.2021.06.018

摘要/Abstract

摘要： 为筛选对番茄匍柄霉叶斑病具有良好拮抗效果的生防细菌，本研究采用稀释涂布法，从四川番茄根际土壤分离筛选到一株对番茄匍柄霉Stemphylium lycopersici（Enjoji）Yamamoto具有显著抑制效果的生防菌株YS05。经过形态学特征观察、生理生化特征、Biolog测定和多基因系统发育树分析，鉴定其为绿针假单胞菌桔黄亚种Pseudomonas chlororaphis subsp.aurantiaca。生防机理初步研究发现，菌株YS05在代谢过程中可产生蛋白酶、氢氰酸、吩嗪-1-羧酸（phenazine-1-carboxilic acid）和硝吡咯菌素（pyrrolnitrin），具有广谱拮抗作用，能够有效抑制9种病原真菌和3种病原细菌的生长。采用二分皿法测定其挥发性物质对7种病原菌均有抑制效果。离体叶片和活体盆栽条件下，接种菌株YS05的番茄发病率和病情指数均显著降低，离体叶片防效可达71.52%，活体盆栽防效可达61.27%。综上所述，菌株YS05具有良好的生防潜力和应用前景。

关键词: 绿针假单胞菌桔黄亚种, 番茄匍柄霉叶斑病, 番茄匍柄霉, 生物防治

Abstract: In order to obtain the antagonistic strain with stronger inhibition and biocontrol effect on gray leaf spot of tomato. In this study, the biocontrol bacterium YS05 isolated from tomato field soil samples had significant inhibitory effect on Stemphylium lycopersici (Enjoji) Yamamoto by gradient dilution plate method. The strain YS05 was identified as Pseudomonas chlororaphis subsp. aurantiaca based on biological characteristics, physiological and biochemical characteristics, and multilocus sequence analysis. The results of biological characters assay show that strain YS05 could produce protease, HCN, phenazine-1-carboxilic acid and pyrrolnitrin during the metabolic process, and exhibited broad inhibitory spectrum against nine pathogenic fungi and three pathogenic bacteria. Under the condition of in vitro leaves and potted plants in greenhouse, the results of biocontrol study show that the disease incidence and index of tomato inoculated with strain YS05 were significantly reduced. The control efficacy was 71.40% and 58.27%, respectively. In summary, the strain YS05 has excellent biocontrol characteristics and potential in biocontrol of vegetable diseases.

Key words: Pseudomonas chlororaphis subsp. aurantiaca, gray leaf spot of tomato, Stemphylium lycopersici (Enjoji) Yamamoto, biological control

中图分类号:

S476

黄艺烁, 谢学文, 石延霞, 柴阿丽, 李磊, 李宝聚. 一株绿针假单胞菌桔黄亚种在防治番茄匍柄霉叶斑病中的应用[J]. 中国生物防治学报, 2021, 37(6): 1265-1275.

HUANG Yishuo, XIE Xuewen, SHI Yanxia, CHAI Ali, LI Lei, LI Baoju. Application of Pseudomonas chlororaphis subsp. aurantiaca against Gray Leaf Spot of Tomato[J]. Chinese Journal of Biological Control, 2021, 37(6): 1265-1275.

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