假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应

doi:10.16409/j.cnki.2095-039x.2016.02.002

中国生物防治学报

假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应

韩善捷, 潘铖, 韩宝瑜

中国计量学院生命科学学院/浙江省生物计量及检验检疫技术重点实验室, 杭州 310018

收稿日期:2015-05-15 出版日期:2016-04-08 发布日期:2016-04-08
通讯作者: 韩宝瑜
作者简介:韩善捷(1992-),男,硕士研究生,E-mail:hanshanjie@126.com
基金资助:
国家自然科学基金(31071744);杭州市科技计划(20140533B01);浙江省大学生创新创业(2014R409056)

Changes in Volatiles of Tea Shoots Damaged by Tea Green Leafhoppers and Their Attraction to Schizophragma parvula Ogloblin

HAN Shanjie, PAN Cheng, HAN Baoyu

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences of China Jiliang University, Hangzhou 310018, China

Received:2015-05-15 Online:2016-04-08 Published:2016-04-08

摘要/Abstract

摘要： 以SDE法提取健康茶梢、假眼小绿叶蝉为害茶梢挥发物,用GC-MS配合标准样品从二者中鉴定了17种共有成分,包括顺-3-己烯-1-醇、反-2-己烯醛、芳樟醇、香叶醇、β-紫罗酮和橙花叔醇等常见茶叶香气成分。从前者另外检出顺-3-己烯基丁酸酯等9种成分,还从后者另外测出了己醛等7种成分。假眼小绿叶蝉为害茶梢挥发物各组分总含量、健康茶梢挥发物各组分总含量相对于内标含量的比例分别是1783.8%和360.3%。从蝉害茶梢测出了丰富的反-2-己烯醛、苯甲醛、α-法呢烯和水杨酸甲酯,4种成分占蝉害茶梢、健康茶梢挥发物总量分别为11.8%和8.3%;室内以蝉害茶梢19种主要挥发物的10^-2 g/mL剂量为味源,液体石蜡为CK,以Y管嗅觉仪进行行为测定,结果表明这4种成分皆显著引诱微小裂骨缨小蜂(P<0.05)。认为蝉害改变了茶梢挥发性化合物的组成及其相对含量,尤其是产生的反-2-己烯醛、苯甲醛、α-法呢烯和水杨酸甲酯等互利素显著地引诱该叶蝉的卵寄生蜂微小裂骨缨小蜂。

关键词: 假眼小绿叶蝉, 微小裂骨缨小蜂, 互利素, 茶梢挥发物, 引诱

Abstract: The volatile compounds from healthy intact tea shoots (ITS) and the tea green leafhopper Empoasca vitis (Göthe) damaged tea shoots (TGLDTS) were collected by simultaneous distillation extraction (SDE) method, and identified by gas chromatography-mass spectrometry and further confirmed by comparison with synthetic standard samples. Seventeen volatile components shared by both ITS and TGLDTS were identified, including Z-3-hexen-1-ol, E-2-hexenal, linalool, geraniol, β-ionone and nerolidol and other common aromatic compounds. Nine other components including Z-3-hexenyl butyrate were identified only from ITS; whereas, additional seven components including hexanal were detected only from TGLDTS samples. The total volatile contents from TGLDTS and ITS samples were estimated to be 17.84 and 3.6 times as much as the internal standard, respectively. E-2-hexenal, benzaldehyde, α-farnesene and methyl salicylate were four abundant volatile components from TGLDTS, accounting for 11.8% and 8.3% of total volatile contents of TGLDTS and ITS, respectively. Nineteen synthetic volatile components, identified from TGLDTS, each at a dosage of 10^-2 g/mL, were tested in the lab Y-tube olfactometer against a mymarid parasitoid (Schizophragma parvula Ogloblin). Our bioassay results clearly showed that four of the tested compounds from TGLDTS E-2-hexenal, benzaldehyde, α-farnesene and methyl salicylate were significantly attractive to the mymarid. The feeding damage by tea green leafhopper seemed to increase the releases of E-2-hexenal, benzaldehyde, α-farnesene and methyl salicylate that may act as synomone components attracting the egg parastoid of the tea green leafhopper, S. parvula.

Key words: tea green leafhopper, Schizophragma parvula, synomone, tea shoot volatiles, attraction

中图分类号:

S476.3

韩善捷, 潘铖, 韩宝瑜. 假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应[J]. 中国生物防治学报, DOI: 10.16409/j.cnki.2095-039x.2016.02.002.

HAN Shanjie, PAN Cheng, HAN Baoyu. Changes in Volatiles of Tea Shoots Damaged by Tea Green Leafhoppers and Their Attraction to Schizophragma parvula Ogloblin[J]. journal1, DOI: 10.16409/j.cnki.2095-039x.2016.02.002.

参考文献

[1] Sobhy I S, Erb M, Turlings T C J. Plant strengtheners enhance parasitoid attraction to herbivore-damaged cotton via qualitative and quantitative changes in induced volatiles[J]. Pest Management Science, 2015, 71(5):686-693.
[2] Turlings T C J, Loughrin J H, MeCall P J, et al. How caterpillar-damaged plants protect themselves by attracting parasitic wasps[J]. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92(10):4169-4174.
[3] Dicke M. Evolution of induced in direct defense of plant//Tollrian R, Harvell C D, eds. The Ecology and Evolution of Inducible Defenses[M]. Princeton:Princeton University Press, 999, 62-88.
[4] DeMoraes C M, Lewis W J, Par P W, et al. Herbivore-infested plants selectively attract parasitoids[J]. Nature, 1998, 393(6685):570-573.
[5] Pare P W, Tumlinson J H. Cotton volatiles synthesized and released distal to the site of insect damage[J]. Phytochemistry, 1998, 47(4):521-526.
[6] Socorro A P D, Gregg P C, Alter D, et al. Development of a synthetic plant volatile-based attracticide for female noctuid moths. I. potential sources of volatiles attractive to Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)[J]. Australian Journal of Entomology, 2010, 49(1):10-20.
[7] Peter C G, Socorro A P D, Henderson G S. Development of a synthetic plant volatile-based attracticide for female noctuid moths. II. Bioassays of synthetic plant volatiles as attractants for the adults of the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)[J]. Australian Journal of Entomology, 2010, 49(1):21-30.
[8] Socorro A P D, Gregg P C, Hawes A J. Development of a synthetic plant volatile-based attracticide for female noctuid moths. III. Insecticides for adult Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)[J]. Australian Journal of Entomology, 2010, 49(1):31-39.
[9] 韩宝瑜, 林金丽, 周孝贵, 等. 假眼小绿叶蝉卵及卵寄生蜂缨小蜂形态观查和寄生率考评[J]. 安徽农业大学学报, 2009, 36(1):13-17.
[10] 李慧玲, 林乃铨. 假眼小绿叶蝉卵缨小蜂的生物学特性研究[J]. 茶叶科学, 2008, 28(6):407-413.
[11] 毛迎新, 邹武, 马新华, 等. 假眼小绿叶蝉卵的寄生蜂种类及种群动态[J]. 昆虫知识, 2008, 45(3):472-474.
[12] 毛迎新, 谭荣荣, 龚自明, 等. 武汉地区茶园假眼小绿叶蝉及卵寄生蜂种群消长动态[J]. 湖北农业科学, 2013, 52(4):823-825.
[13] Han B Y, Chen Z M. Behavioral and electrophysiological responses of natural enemies to synomones from tea shoots and kairomones from tea aphids, Toxoptera aurantii[J]. Journal of Chemical Ecology, 2002, 28(11):2203-2219.
[14] 黄毅, 韩宝瑜, 唐茜, 等. 茶尺蠖绒茧蜂对茶梢挥发物的EAG和行为反应[J]. 昆虫学报, 2009, 52(11):1191-1198.
[15] Jetske G, de Boer, Marcel D. The role of methyl salicylate in prey searching behavior of the predatory mite Phytoseiulus persimilis[J]. Journal of Chemical Ecology, 2004, 30(2):255-271.
[16] James D G, Price T S. Field-testing of methyl salicylate for recruitment and retention of beneficial insects in grapes and hops[J]. Journal of Chemical Ecology, 2004, 30(8):1613-1628.
[17] 李名君. 茶叶化学//陈宗懋主编. 中国茶叶大辞典[M]. 北京:中国轻工业出版社, 2000, 323-359.
[18] Nadia Zekri N, Amalich S, Elbelghiti M A, et al. Phytochemical screening and chemical composition of essential oils and hydrosols of Mentha species from Morocco[J]. Advances in Environmental Biology, 2014, 8(17):10-18.
[19] Mu D, Cui L, Ge J, et al. Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper, Empoaca vitis[J]. Insect Science, 2012, 19(2):229-238.

假眼小绿叶蝉为害致茶梢挥发物变化及其引诱微小裂骨缨小蜂效应

Changes in Volatiles of Tea Shoots Damaged by Tea Green Leafhoppers and Their Attraction to Schizophragma parvula Ogloblin

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

联系我们

[1]	蔡晓明, 边磊, 李兆群, 罗宗秀, 修春丽, 陈宗懋. 田间背景气味对植物源引诱剂的干扰及相应研发策略[J]. 中国生物防治学报, 2020, 36(5): 646-655.
[2]	范培珍, 韩善捷, 韩宝瑜. 灰茶尺蠖为害诱导茶树释放的互利素的鉴定[J]. 中国生物防治学报, 2020, 36(1): 65-71.
[3]	杨苗苗, 李英梅, 张淑莲, 洪波, 王晶玲, 陈志杰. 烟粉虱MED隐种对植物挥发物的趋性行为[J]. 中国生物防治学报, 2018, 34(5): 663-669.
[4]	马娟, 王容燕, 李秀花, 高波, 问海月, 陈书龙. 甘薯对马铃薯腐烂茎线虫趋化性的影响[J]. 中国生物防治学报, 2018, 34(1): 141-147.
[5]	王海涛, 于娜, 王丹凤, 侯瑞, 刘振宇, 卢希平. 几种无公害技术对褐梗天牛的防治效果[J]. 中国生物防治学报, 2016, 32(5): 666-671.
[6]	李慧玲, 林乃铨, 郭剑雄, 王水金, 王庆森, 曾明森, 吴光远. 茶园间作绿肥对假眼小绿叶蝉及其天敌缨小蜂的影响[J]. 中国生物防治学报, 2016, 32(1): 50-54.
[7]	王新花1 , 庞献伟1 , 李冬梅1 , 朱翠英2 , 单长松3 , 王晓英1 . 一种缓释型双条杉天牛引诱剂缓释效果的研究[J]. , 2015, 31(3): 416-422.
[8]	马艳粉1,2 , 胥勇3 , 肖春2 . 10种寄主植物挥发物对马铃薯块茎蛾产卵的引诱作用[J]. , 2012, 28(3): 448-452.
[9]	展茂魁, 何玲敏, 陈名, 王滨, 李增智. 防治假眼小绿叶蝉的虫生真菌高毒菌株的筛选及田间防治效果[J]. , 2012, 28(1): 41-46.
[10]	王滨1,2 ;樊美珍2 ;李增智2 . 白僵菌无纺布结合引诱剂防治鞘翅目林业害虫研究初报 [J]. , 2003, 19(2): 91-92.
[11]	张庆贺1 ;陈国发1 ;赵晋龙2 . 华北落叶松鞘蛾性引诱剂及其应用技术研究[J]. , 1995, 11(4): 145-149.
[12]	陈银方. 浙江省山地茶园蜘蛛研究初报[J]. , 1992, 8(2): 68-71.
[13]	刘孟英1 ; 潘恒1 ; 伍德明1 ; 苏荣辉1 ; 孟和平2 . 合成性诱剂对粘虫雄蛾的引诱活性[J]. , 1985, 1(2): 1-5.