水稻防御反应相关基因对稻瘟病菌的响应

doi:10.16409/j.cnki.2095-039x.2017.04.010

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (4): 504-511.DOI: 10.16409/j.cnki.2095-039x.2017.04.010

水稻防御反应相关基因对稻瘟病菌的响应

刘潮^1,2, 王慧¹, 刘林¹, 杨静¹, 李成云¹

1. 云南农业大学/省部共建云南生物资源保护与利用国家重点实验室, 昆明 650201;
2. 曲靖师范学院/云南高原生物资源保护与利用研究中心/云南省高校云贵高原动植物多样性及生态适应性进化重点实验室, 曲靖 655011

收稿日期:2017-01-13 出版日期:2017-08-08 发布日期:2017-08-11
通讯作者: 李成云,教授,E-mail:licheng_yun@163.com
作者简介:刘潮,讲师,E-mail:liuchao@mail.qjnu.edu.cn
基金资助:
国家重点研发计划（2012CB722901）

Response of Rice Defense-related Genes to Blast Fungus

LIU Chao^1,2, WANG Hui¹, LIU Lin¹, YANG Jing¹, LI Chengyun¹

1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan/Yunnan Agricultural University, Kunming 650201, China;
2. Center for Yunnan Plateau Biological Resources Protection and Utilization/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on YunGui Plateau/Qujing Normal University, Qujing 655011, China

Received:2017-01-13 Online:2017-08-08 Published:2017-08-11

摘要/Abstract

摘要： 水稻与稻瘟病菌的互作已成为研究植物与病原真菌互作的模式系统。利用RT-PCR技术检测了6个水稻品种（分别含有抗病基因Pik-s、Pita、Pit、Pi1、Pi9的近等系及回交亲本丽江新团黑谷LTH）与稻瘟病菌互作过程中多个信号相关及PR基因的表达。结果表明带有稻瘟病抗性基因Pik-s、Pita、Pit的水稻品种和LTH对稻瘟病菌#626侵染表现为亲和互作，带Pi1和Pi9的水稻品种表现为非亲和互作；稻瘟病菌接种后，亲和互作中MAPK6和MAPK12表现为上调表达，带有抗性基因Pi9的水稻品种IRBL22中BIMK2表现为上调表达。总体来看，含有不同抗病基因的水稻近等系中的PR基因对稻瘟病菌的响应较为多样，非亲和互作中在早期或早中期表现为PR基因上调表达，而亲和互作中主要在晚期上调表达，说明这些PR基因表达的时间在植物与病原互作的不同时期发挥着不同的作用。

关键词: 稻瘟病, 防御相关基因, 水稻近等基因系, 互作

Abstract: The rice-Magnaporthe oryzae pathosystem has become a model in the study of plant-fungal interactions. The expression of several signal associated and PR genes were analyzed in six rice lines (harboring resistance genes Pik-s, Pita, Pit, Pi1, Pi9 respectively with the backcross parent LTH) under M. oryzae infection by RT-PCR. The results showed that the rice lines harboring blast resistance gene Pik-s, Pita, Pit were compatible interaction with M. oryzae #626, the rice lines harboring Pi1 and Pi9 were incompatible interaction. The expression of MAPK12 and MAPK6 were up-regulated under susceptible interaction. BIMK2 showed higher expression under Pi9 mediated incompatible interaction. In conclusion, the PR genes expression pattern was more diversity to M. oryzae infection in harboring different resistance genes in rice monogenic lines. The up-regulation response of PR genes appeared in the early infection period in incompatible interactions, and at the late period in compatible interactions, indicating that the temporal expression pattern of these PR genes play an important role in plant pathogen interactions.

Key words: rice blast disease, defense-related gene, rice near isogenic line, interaction

中图分类号:

S435.11

刘潮, 王慧, 刘林, 杨静, 李成云. 水稻防御反应相关基因对稻瘟病菌的响应[J]. 中国生物防治学报, 2017, 33(4): 504-511.

LIU Chao, WANG Hui, LIU Lin, YANG Jing, LI Chengyun. Response of Rice Defense-related Genes to Blast Fungus[J]. journal1, 2017, 33(4): 504-511.

参考文献

[1] van Loon L C, Rep M, Pieterse C M. Significance of inducible defense-related proteins in infected plants[J]. Annual Review of Phytopathology, 2006, 44:135-162.
[2] Yuan Y, Zhong S, Li Q, et al. Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility[J]. Plant Biotechnology Journal, 2007, 5(2):313-324.
[3] Kaku H, Nishizawa Y, Ishii-Minami N, et al. Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor[J]. Proceedings of the National Academy of Sciences, 2006, 103(29):11086-11091.
[4] Liu B, Li J F, Ao Y, et al. Lysin motif-containing proteins LYP4 and LYP6 play dual roles in peptidoglycan and chitin perception in rice innate immunity[J]. The Plant Cell, 2012, 24(8):3406-3419.
[5] Sinha A K, Jaggi M, Raghuram B, et al. Mitogen-activated protein kinase signaling in plants under abiotic stress[J]. Plant Signaling and Behavior, 2011, 6(2):196-203.
[6] Adachi H, Ishihama N, Nakano T, et al. Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea[J].Plant Signaling and Behavior, 2016, 11(6):e1183085.
[7] Birkenbihl R P, Kracher B, Somssich I E. Induced genome-wide binding of three Arabidopsis WRKY transcription factors during early MAMP-triggered immunity[J]. The Plant Cell, 2017, 29(1):20-38.
[8] Shimono M, Koga H, Akagi A Y A, et al. Rice WRKY45 plays important roles in fungal and bacterial disease resistance[J]. Molecular Plant Pathology, 2012, 13(1):83-94.
[9] Pandey S P, Somssich I E. The role of WRKY transcription factors in plant immunity[J]. Plant Physiology, 2009, 150(4):1648-1655.
[10] Wang H, Hao J, Chen X, et al. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants[J]. Plant Molecular Biology, 2007, 65(6):799-815.
[11] Breen S, Williams S J, Winterberg B, et al. Wheat PR-1 proteins are targeted by necrotrophic pathogen effector proteins[J]. The Plant Journal, 2016, 88(1):13-25.
[12] Bufe A, Spangfort M D, Kahlert H, et al. The major birch pollen allergen, Bet v 1, shows ribonuclease activity[J]. Planta, 1996, 199(3):413-415.
[13] Liu C, Cheng F, Sun Y, et al. Structure-function relationship of a novel PR-5 protein with antimicrobial activity from soy Hulls[J]. Journal of Agricultural and Food Chemistry, 2016, 64(4):948-959.
[14] Mahdavi F, Sariah M, Maziah M. Expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to Fusarium wilt[J]. Applied Biochemistry and Biotechnology, 2012, 166(4):1008-1019.
[15] Misra R C, Sandeep M K, Kumar S, et al. A thaumatin-like protein of Ocimum basilicum confers tolerance to fungal pathogen and abiotic stress in transgenic Arabidopsis[J]. Scientific Reports, 2016, 6:25340
[16] 杨祁云, 朱小源, 雷财林, 等. 华南籼稻稻瘟病菌致病型单基因鉴别寄主筛选[J]. 植物保护学报, 2004, 31(2):113-120.
[17] 李进斌, 李成云, 张庆, 等. 二十二个抗稻瘟病基因在云南的利用价值评价[J]. 植物保护学报, 2005, 32(2):113-119.
[18] 杨健源, 陈深, 曾列先, 等. 稻瘟病主效抗性基因对广东省籼稻稻瘟病菌的抗性评价[J]. 中国水稻科学, 2008, 22(2):190-196.
[19] Shimizu T, Nakano T, Takamizawa D, et al. Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice[J]. The Plant Journal, 2010, 64(2):204-214.
[20] Kim S H, Oikawa T, Kyozuka J, et al. The bHLH Rac immunity1(RAI1) is activated by OsRac1 via OsMAPK3 and OsMAPK6 in rice immunity[J]. Plant and Cell Physiology, 2012, 53(4):740-754.
[21] Lieberherr D, Thao N P, Nakashima A, et al. A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice[J]. Plant Physiology, 2005, 138(3):1644-1652.
[22] Yoo S D, Cho Y H, Tena G, et al. Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling[J]. Nature, 2008, 451(7180):789-795.
[23] Kawano Y, Akamatsu A, Hayashi K, et al. Activation of a Rac GTPase by the NLR family disease resistance protein Pit plays a critical role in rice innate immunity[J]. Cell Host and Microbe, 2010, 7(5):362-375.

水稻防御反应相关基因对稻瘟病菌的响应

Response of Rice Defense-related Genes to Blast Fungus

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

联系我们

[1]	荆玉玲, 郭荣君, 李世东. 黄瓜枯萎病菌菌丝际细菌种群丰度变化及其影响黄瓜生长的原因初探[J]. 中国生物防治学报, 2020, 36(5): 751-760.
[2]	朱峰, 王继春, 田成丽, 祁山颜, 王东元, 任金平, 王义山. 枯草芽胞杆菌GB519抗菌蛋白的理化性质及生防效果[J]. 中国生物防治学报, 2020, 36(5): 778-785.
[3]	吴希阳, 罗路云, 谭新球, 张德咏, 陈岳, 刘勇. 沼泽红假单胞菌Atp2蛋白的原核表达及稻瘟病菌的互作蛋白初步筛选[J]. 中国生物防治学报, 2020, 36(3): 421-428.
[4]	徐佳, 金武, 陈春. 有翅桃蚜蚜群中新蚜虫疠霉和烟蚜茧蜂的互作关系初探[J]. 中国生物防治学报, 2020, 36(3): 437-442.
[5]	周瑚, 胡玲, 余曦玥, 朱华珺, 任佐华, 刘二明. 特基拉芽胞杆菌JN-369抗菌粗蛋白的理化性质和抑菌效果[J]. 中国生物防治学报, 2020, 36(2): 211-219.
[6]	沙月霞, 张昂, 伍顺华, 沈瑞清. 防治稻瘟病假单胞菌的筛选及效果评价[J]. 中国生物防治学报, 2020, 36(2): 249-257.
[7]	吴圣勇, 杨清坡, 徐长春, 徐学农, 雷仲仁. 昆虫病原真菌和捕食螨间的互作关系及二者联合应用研究进展[J]. 中国生物防治学报, 2019, 35(1): 127-133.
[8]	王玉双, 肖蓉, 郭照辉, 曾奥, 付祖姣, 杨华, 罗容珺, 胡展, 单世平, 雷平. 来源于中药的稻瘟病菌拮抗解淀粉芽胞杆菌鉴定及其活性成分特性[J]. 中国生物防治学报, 2018, 34(5): 746-752.
[9]	马佳, 李颖, 胡栋, 彭杰丽, 贾楠, 张翠绵, 王旭, 王占武. 芽胞杆菌生物防治作用机理与应用研究进展[J]. 中国生物防治学报, 2018, 34(4): 639-648.
[10]	张荣胜, 王法国, 齐中强, 杜艳, 刘永锋. 生防菌解淀粉芽胞杆菌Jt84干悬浮剂加工及田间评价[J]. 中国生物防治学报, 2018, 34(3): 407-413.
[11]	沙月霞, 曾庆超, 王昕, 沈瑞清, 刘浩, 王喜刚. 防治稻瘟病芽胞杆菌的筛选及效果评价[J]. 中国生物防治学报, 2018, 34(3): 414-422.
[12]	甘玉姿, 肖贵, 邓启云, 吴俊, 柏斌, 卢向阳, 周波. 菲律宾稻瘟病菌生理小种中AVR-Pita及其同源基因的序列与功能分析[J]. 中国生物防治学报, 2018, 34(3): 488-498.
[13]	王法国, 许萍萍, 张荣胜, 陆凡, 刘永锋, 李虹. 解淀粉芽胞杆菌干悬浮剂对稻瘟病的防治效果及安全性评价[J]. 中国生物防治学报, 2017, 33(2/3): 241-247.
[14]	徐文, 黄媛媛, 黄亚丽, 贾振华, 宋水山. 木霉-植物互作机制的研究进展[J]. 中国生物防治学报, 2017, 33(2/3): 408-414.
[15]	邹秋霞, 任佐华, 高诗涵, 周瑚, 赵建辉, 刘二明. 枯草芽胞杆菌YN145分离鉴定及抑菌活性[J]. 中国生物防治学报, 2017, 33(2/3): 421-426.