防治稻瘟病芽胞杆菌的筛选及效果评价

doi:10.16409/j.cnki.2095-039x.2018.03.008

中国生物防治学报 ›› 2018, Vol. 34 ›› Issue (3): 414-422.DOI: 10.16409/j.cnki.2095-039x.2018.03.008

防治稻瘟病芽胞杆菌的筛选及效果评价

沙月霞¹, 曾庆超², 王昕³, 沈瑞清¹, 刘浩¹, 王喜刚¹

1. 宁夏农林科学院植物保护研究所, 银川 750011;
2. 中国农业大学植物保护学院, 北京 100193;
3. 宁夏农林科学院农作物研究所, 永宁 750105

收稿日期:2017-12-13 出版日期:2018-06-08 发布日期:2018-06-14
通讯作者: 沙月霞,博士,副研究员,E-mail:yuexiasha@126.com
作者简介:沙月霞,博士,副研究员,E-mail:yuexiasha@126.com
基金资助:
宁夏农林科学院科技先导资金项目（NKYJ-16-26）；宁夏自然科学基金项目（NZ17120）；国家重点研发专项（2017YFD0201600）

Screening and Control Efficiency Evaluation of Bacillus against Rice Blast Magnaporthe oryzae

SHA Yuexia¹, ZENG Qingchao², WANG Xin³, SHEN Ruiqing¹, LIU Hao¹, WANG Xigang¹

1. Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750011, China;
2. College of Plant Protection, China Agricultural University, Beijing 100193, China;
3. Institute of Crop Research, Ningxia Academy of Agriculture and Forestry Sciences, Yongning 750105, China

Received:2017-12-13 Online:2018-06-08 Published:2018-06-14

摘要/Abstract

摘要： 从232株细菌中筛选获得2株对稻瘟病菌具有明显抑菌活性的菌株S09和S170，对稻瘟病菌Magnaporthe oryzae P131菌丝的抑制率分别为90.44%和92.38%；离体叶片法测定表明，菌株S09和S170抑菌效果分别为74.62%和75.52%，与绿地康1号无显著差异。16S rDNA序列分析比对，菌株S09与短小芽胞杆菌Bacillus pumilus基因序列的同源性最高；gyrA基因测序结果表明，菌株S170与解淀粉芽胞杆菌B.amyloliquefaciens基因序列的同源性最高。结合形态观察，确定菌株S09为短小芽胞杆菌，菌株S170为解淀粉芽胞杆菌。菌株S09和S170对稻瘟病的温室防治效果分别是76.56%和80.57%。田间试验结果表明，菌株S09和S170对水稻有明显的促生和增产作用，分别提高水稻种子发芽17.37%和12.34%、促进根长18.68%和33.85%、增加株高12.44%和28.49%、降低空秕率25.17%和35.59%，还可以有效改善稻米的垩白粒率和垩白度。短小芽胞杆菌S09和解淀粉芽胞杆菌S170对叶瘟的田间防治效果分别是70.59%和73.19%，对穗颈瘟的田间防治效果分别是71.55%和74.82%，菌株S170对稻瘟病的防治效果与75%三环唑可湿性粉剂、绿地康1号处理无显著差异。

关键词: 稻瘟病, 芽胞杆菌, 筛选, 生物防治, 田间防效

Abstract: 232 bacterial strains were isolated from rice rhizosphere of cultivar Ningjin NO. 48 in Ningxia Hui Autonomous Region. Only two isolates, S09 and S170 strongly inhibited the growth of Magnaporthe oryzae in dual culture technique and leaf blade in vitro. The hyphal growth inhibition rate of strains S09 and S170 on M. oryzae cultured after 4 d were 90.44% and 92.38%, respectively. The control efficacies in leaf blades were 74.62% and 75.52%, respectively, without significant difference from that of Lvdikang NO. 1. 16S rDNA sequence analysis indicated that strain S09 had the highest homology with B. pumilus. gyrA gene sequence analysis indicated that S170 had the highest homology with B. amyloliquefacien. Combining with its the morphological characteristic, the strains S09 and S170 were identified as B. pumilus and B. amyloliquefaciens, respectively. The control efficacies of strains S09 and S170 against rice blast were 76.56% and 80.57% under greenhouse condition, respectively. Field trials showed that strains S09 and S170could promote rice growth, increase the germination rate by 17.37% and 12.34%, promote rice root length by 18.68% and 33.85%, enhance rice plant height by 12.44% and 28.49%, and reduce empty-grain percentage by 25.17% and 35.59%, respectively. Moreover, both trains could improve chalky grain rate and chalkiness ratio. Field control efficacies of B. pumilus S09 and B. amyloliquefaciens S170 were 70.59% and 73.19% against leaf blast, and 71.55% and 74.82% against neck blast, respectively. The control efficacy of strain S170 had no significant difference with that of 75% tricyclazole wettable powder or Lvdikang NO. 1.

Key words: rice blast, Bacillus, screen, bio-control, field efficacy

中图分类号:

S476

沙月霞, 曾庆超, 王昕, 沈瑞清, 刘浩, 王喜刚. 防治稻瘟病芽胞杆菌的筛选及效果评价[J]. 中国生物防治学报, 2018, 34(3): 414-422.

SHA Yuexia, ZENG Qingchao, WANG Xin, SHEN Ruiqing, LIU Hao, WANG Xigang. Screening and Control Efficiency Evaluation of Bacillus against Rice Blast Magnaporthe oryzae[J]. journal1, 2018, 34(3): 414-422.

参考文献

[1] Muthayya S, Sugimoto J D, Montgomery S, et al. An overview of global rice production, supply, trade, and consumption[J]. Annals of the New York Academy of Sciences, 2014, 1324(1):7-14.
[2] Yasin F D, Kae Y, Gulay D. Septin-Mediated plant cell invasion by the rice blast fungus, Magnaporthe oryzae[J]. Science, 2012, 446(22):1589-1595.
[3] Spence C, Alff E, Johnson C, et al. Natural rice rhizospheric microbes suppress rice blast infections[J]. BMC Plant Biology, 2014, 14(1):130.
[4] Saikia R, Gogoi D K, Mazumder S, et al. Brevibacillus laterosporus strain BPM3, a potential biocontrol agent isolated from a natural hot water spring of Assam, India[J]. Microbiological Research, 2011, 166(3):216-225.
[5] Suryadi Y, Susilowati D N, Riana E, et al. Management of rice blast disease (Pyricularia oryzae) using formulated bacterial consortium[J]. Emirates Journal of Food and Agriculture, 2013, 25(5):349-357.
[6] Suryadi Y, Susilowati D N, Kadir T S, et al. Bioformulation of antagonistic bacterial consortium for controlling blast, sheath blight and bacterial blight diseases on rice[J]. Asian Journal of Plant Pathology, 2013, 7(3):92-108.
[7] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[8] 迟莉. 水稻稻瘟病拮抗菌筛选及施用后对水稻植株的影响[D]. 北京:中国农业科学院, 2014.
[9] FAO. The State of Food Insecurity in the World 2012:Economic growth is necessary but not sufficient to accelerate reduction of hunger and malnutrition[R]. Rome:Food and Agriculture Organization of the United Nations, 2012.
[10] 张芬, 刘邮洲, 于俊杰, 等. 水稻稻瘟病菌拮抗细菌的筛选与鉴定[J]. 江苏农业学报, 2011, 27(3):505-509.
[11] Meng X K, Yu J J, Yu M N, et al. Dry flowable formulations of antagonistic Bacillus subtilis strain T429 by spray drying to control rice blast disease[J]. Biological Control, 2015, 85(1):46-51.
[12] Shan H Y, Zhao M M, Chen D, et al. Biocontrol of rice blast by the phenaminomethylacetic acid producer of Bacillus methylotrophicus strain BC79[J]. Crop Protection, 2013, 44(1):29-37.
[13] 王琦, 沙月霞, 李燕, 等. 一株防治稻瘟病的枯草芽孢杆菌2012SYX04:中国, 201410357380.4[P]. 2014-12-10.
[14] 王琦, 沙月霞, 李燕, 等. 用于防治稻瘟病的枯草芽孢杆菌:中国, 201410356669.4[P]. 2014-11-26.
[15] 沙月霞, 王琦, 李燕. 稻瘟病生防芽胞杆菌的筛选及防治效果[J]. 中国生物防治学报, 2016, 32(4):474-484.
[16] Ng L C, Sariah M, Sariam O, et al. Bio-efficacy of microbial-fortified rice straw compost on rice blast disease severity, growth and yield of aerobic rice[J]. Australasian Plant Pathology, 2012, 41(5):541-549.
[17] Zhang C X, Zhang X X, Shen S H. Proteome analysis for antifungal effects of Bacillus subtilis KB-1122 on Magnaporthe grisea P131[J]. World Journal of Microbiology and Biotechnology, 2014, 30(6):1763-1774.
[18] Sha Y X, Wang Q, Li Y. Suppression of Magnaporthe oryzae and interaction between Bacillus subtilis and rice plants in the control of rice blast[J]. SpringerPlus, 2016, 5(1):1238.
[19] Gutierrez-Manero F J, Ramos-Solano B, Probanza A, et al. The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins[J]. Physiologla Plantarum, 2001, 111(2):206-211.
[20] Ryu H Y, Kim S Y, Park H M, et al. Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death[J]. Biochemical and Biophysical Research Community, 2009, 379(2):417-422.
[21] Wang W D, Chen L N, Wu H, et al. Comparative proteomic analysis of rice seedlings in response to inoculation with Bacillus cereus[J]. Letters in Applied Microbiology, 2013, 56(3):208-215.
[22] Chandler S, Van Hese N, Coutte F, et al. Role of cyclic lipopeptides produced by Bacillus subtilis in mounting induced immunity in rice (Oryza sativa L.)[J]. Physiological and Molecular Plant Pathology, 2015, 91:20-30.
[23] Idris E E, Iglesias D J, Talon M, et al. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42[J]. Molecular Plant-Microbe Interactions, 2007, 20(6):619-626.
[24] Saleem M, Arshad M, HussainS, et al. Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture[J]. Journal of Industrial Microbiology and Biotechnology, 2007, 34(10):635-648.

防治稻瘟病芽胞杆菌的筛选及效果评价

Screening and Control Efficiency Evaluation of Bacillus against Rice Blast Magnaporthe oryzae

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

联系我们

[1]	阿尔孜姑丽·肉孜, 吐尔逊·阿合买提, 付开赟, 丁新华, 阿地力·沙塔尔, 郭文超. 新疆玉米种植区瓢虫资源调查及多样性分析[J]. 中国生物防治学报, 2020, 36(5): 697-707.
[2]	孙元星, 郝亚楠, 李明凌. 贮藏前补充人工饲料对七星瓢虫低温耐受力的影响[J]. 中国生物防治学报, 2020, 36(5): 708-713.
[3]	饶文华, 占亚婷, 方云, 游雨欣, 聂丹玥, 郭雪萍, 张顶洋, 关雄, 潘晓鸿. 生物炭对Bt Cry1Ac蛋白抗紫外能力影响[J]. 中国生物防治学报, 2020, 36(5): 714-720.
[4]	许帅, 谢学文, 张昀, 石延霞, 柴阿丽, 李磊, 李宝聚. 马铃薯枯萎病生防芽胞杆菌筛选及生防效果研究[J]. 中国生物防治学报, 2020, 36(5): 761-770.
[5]	李小杰, 刘畅, 李成军, 姚晨虓, 白静科, 于冰娜, 邱睿, 陈玉国, 李淑君. 多粘类芽胞杆菌LB-9的诱变及抑菌防病效果研究[J]. 中国生物防治学报, 2020, 36(5): 771-777.
[6]	朱峰, 王继春, 田成丽, 祁山颜, 王东元, 任金平, 王义山. 枯草芽胞杆菌GB519抗菌蛋白的理化性质及生防效果[J]. 中国生物防治学报, 2020, 36(5): 778-785.
[7]	何伟, 罗文芳, 许建军, 孙晓军. 贝莱斯芽胞杆菌JTB8-2的筛选、鉴定及其对瓜列当拮抗作用研究[J]. 中国生物防治学报, 2020, 36(5): 786-794.
[8]	郭义, 赵灿, 李君摘, 李敦松. 蠋蝽对荔枝蝽一龄若虫的捕食功能反应[J]. 中国生物防治学报, 2020, 36(5): 826-831.
[9]	田俊策, 鲁艳辉, 王国荣, 郑许松, 杨亚军, 徐红星, 方琦, 叶恭银, 臧连生, 吕仲贤. 种赤眼蜂对草地贪夜蛾卵的寄生能力研究[J]. 中国生物防治学报, 2020, 36(4): 485-490.
[10]	路子云, 杨小凡, 马爱红, 冉红凡, 刘文旭, 李建成. 管侧沟茧蜂对不同日龄草地贪夜蛾幼虫的寄生效果[J]. 中国生物防治学报, 2020, 36(4): 491-495.
[11]	杨磊, 李芬, 吴少英. 草地贪夜蛾寄生蜂资源及其调控寄主免疫反应的研究[J]. 中国生物防治学报, 2020, 36(4): 496-506.
[12]	黄潮龙, 汤印, 何康来, 王振营. 双斑青步甲幼虫对草地贪夜蛾幼虫的捕食能力[J]. 中国生物防治学报, 2020, 36(4): 507-512.
[13]	李萍, 李玉艳, 向梅, 王孟卿, 毛建军, 陈红印, 张礼生. 大草蛉幼虫对草地贪夜蛾低龄幼虫的捕食能力评价[J]. 中国生物防治学报, 2020, 36(4): 513-519.
[14]	王亚楠, 赵胜园, 何运转, 吴孔明, 李国平, 封洪强. 黄带犀猎蝽对草地贪夜蛾幼虫的捕食作用[J]. 中国生物防治学报, 2020, 36(4): 525-529.
[15]	张亮, 吕翠, 段彩琛, 王运生, 黄军, 杜杰, 黎定军, 刘清术, 陈武. 转录调控因子AbrB对生防短短芽胞杆菌X23中抗生素edeines生物合成的影响[J]. 中国生物防治学报, 2020, 36(4): 564-574.