马铃薯块茎蛾幼虫致病黏质沙雷氏菌的分离鉴定及杀虫活性

doi:10.16409/j.cnki.2095-039x.2020.03.003

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (3): 361-370.DOI: 10.16409/j.cnki.2095-039x.2020.03.003

马铃薯块茎蛾幼虫致病黏质沙雷氏菌的分离鉴定及杀虫活性

苏造堂¹, 张凌英¹, 徐天梅¹, 杜广祖¹, 陈斌¹, 肖关丽²

1. 云南农业大学植物保护学院/云南生物资源保护与利用国家重点实验室, 昆明 650201;
2. 云南农业大学农学与生物技术学院, 昆明 650201

收稿日期:2019-11-05 出版日期:2020-06-08 发布日期:2020-06-12
通讯作者: 陈斌,教授,E-mail:chbins@163.com;肖关丽,教授,E-mail:glxiao9@163.com。
作者简介:苏造堂,硕士研究生,E-mail:1107636590@qq.com;张凌英,硕士研究生,E-mail:956813024@qq.com
基金资助:
国家自然科学基金（31760519，31660537）

Isolation, Identification and Insecticidal Activity of Pathogenic Serratia marcescens from Potato Tuber Moth Larvae

SU Zaotang¹, ZHANG Lingying¹, XU Tianmei¹, DU Guangzu¹, CHEN Bin¹, XIAO Guanli²

1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan/College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
2. College of Agriculture & Biology Technology, Yunnan Agricultural University, Kunming 650201, China

Received:2019-11-05 Online:2020-06-08 Published:2020-06-12

摘要/Abstract

摘要： 从马铃薯田自然罹病马铃薯块茎蛾幼虫上，分离到1株对马铃薯块茎蛾幼虫具有较强致病作用的菌株ML-1，通过形态及16S rRNA序列测定分析，确定该菌株ML-1为黏质沙雷氏菌Serratia marcescens。采用饲喂法分别测定了该菌株ML-1菌悬液、发酵液及发酵上清液对马铃薯块茎蛾初孵3日龄幼虫的毒力。结果表明，用ML-1菌悬液、发酵液及发酵上清液饲喂后第7 d时，马铃薯块茎蛾3日龄幼虫的累积校正死亡率分别为63.16%、80.70%和49.12%，在3.0×10⁹ cfu/mL浓度下的LT₅₀分别为4.71、3.04和6.47 d，在发酵液处理后第3、5和7 d的LC₅₀分别为9.784×10¹⁰、1.855×10⁸和5.434×10⁵ cfu/mL。综上所述，黏质沙雷氏菌ML-1菌株对马铃薯块茎蛾幼虫有较强的毒力，在马铃薯块茎蛾绿色防控中具有一定的开发潜能。

关键词: 马铃薯块茎蛾, 黏质沙雷氏菌, 毒力测定, 致病力, 杀虫活性

Abstract: A strain ML-1 showing strong pathogenicity to larvae of potato tuber moth was isolated from the natural diseased larvae of potato tuber moth collected from potato fields. The strain ML-1 was identified as S. marcescens by morphological observation of pathogens and 16S rRNA sequencing analysis. The virulence of the strain ML-1 suspension, fermentation broth and fermentation supernatant to the 3-d-old larvae of potato tuber moth was determined by feeding method. The cumulative corrected mortalities were 63.16%, 80.70% and 49.12% on the 7th day after feeding, respectively, and the LT₅₀ were 3.04, 4.71 and 6.47 d at 3.0×10⁹ cfu/mL, respectively. The LC₅₀ at the 3rd, 5th and 7th day after treatment with fermentation broth were 9.784x10¹⁰, 1.855x10⁸ and 5.434x10⁵ cfu/mL, respectively. In summary, S. marcescens ML-1 strain has strong virulence to potato tuber moth larvae and has good prospective for development of biological control agents against the pest.

Key words: potato tuber moth, Serratia marcescens, virulence determination, pathogenicity, insecticidal activity

中图分类号:

S476

苏造堂, 张凌英, 徐天梅, 杜广祖, 陈斌, 肖关丽. 马铃薯块茎蛾幼虫致病黏质沙雷氏菌的分离鉴定及杀虫活性[J]. 中国生物防治学报, 2020, 36(3): 361-370.

SU Zaotang, ZHANG Lingying, XU Tianmei, DU Guangzu, CHEN Bin, XIAO Guanli. Isolation, Identification and Insecticidal Activity of Pathogenic Serratia marcescens from Potato Tuber Moth Larvae[J]. Chinese Journal Of Biological Control, 2020, 36(3): 361-370.

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马铃薯块茎蛾幼虫致病黏质沙雷氏菌的分离鉴定及杀虫活性

Isolation, Identification and Insecticidal Activity of Pathogenic Serratia marcescens from Potato Tuber Moth Larvae

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