桃蚜电压门控钠离子通道基因cDNA克隆及其生物信息学分析

doi:10.16409/j.cnki.2095-039x.2020.03.029

摘要/Abstract

摘要： 克隆获得桃蚜电压门控钠离子通道基因cDNA序列，明确钠离子通道的典型特征，为研究桃蚜抗性分子机理奠定基础。采用实验技术主要有RT-PCR和PCR，克隆桃蚜钠离子通道基因cDNA序列，利用相关软件对其序列进行生物信息学分析。克隆得到两段cDNA序列MpNa_v-1（NCBI登录号：MN124170）和MpNa_v-2（NCBI登录号：MN176136）。MpNa_v-1长度为2945 bp，包括2877 bp的完整开放阅读框，共编码958个氨基酸；MpNa_v-2长度为3546 bp，包括3486 bp的完整开放阅读框，共编码1161个氨基酸。MpNa_v-1和MpNa_v-2共同组成桃蚜的钠离子通道α亚基，MpNa_v-1包含同源结构域Ⅰ和同源结构域Ⅱ，MpNa_v-2包含同源结构域Ⅲ和同源结构域Ⅳ。同源比对发现，桃蚜与豌豆蚜和高粱蚜钠离子通道基因相似度分别高达97.67%和97.65%，所克隆序列包含昆虫钠离子通道α亚基典型特征，具有MFM模块，并含有蚜虫类钠通道特有模块DENS。成功地克隆桃蚜钠离子通道基因，为阐明其对拟除虫菊酯类药剂产生靶标抗性的分子机制奠定基础。

关键词: 桃蚜, 钠离子通道, 基因克隆, 生物信息分析, 系统发育分析

Abstract: The cDNA sequence of the voltage-gated sodium channel gene of Myzus persicae was cloned and the typical characteristics of sodium channel were determined, which laid a foundation for studying the molecular mechanism of resistance to Aphis gossypii. The experimental techniques were mainly RT-PCR and PCR, and the cDNA sequence of the sodium ion channel gene was cloned, and the sequence bioinformatics analysis was carried out by using relevant software. Cloning two cDNA sequences MpNa_v-1 (NCBI accession number:MN124170) and MpNa_v-2 (NCBI accession number:MN176136). MpNa_v-1 is 2945 bp in length and includes a 2877 bp complete open reading frame encoding a total of 958 amino acids. MpNa_v-2 is 3546 bp in length and includes a 3486 bp complete open reading frame encoding a total of 1161 amino acids. MpNa_v-1 and MpNa_v-2 together form the sodium channel of Myzus persicae, MpNa_v-1 contains a homologous domain I and a homologous domain II MpNa_v-2 contains a homology domain III and a homologous domain IV. The homology alignment found that the similarity between the Acyrthosiphon pisum and the Melanaphis sacchari sorghum sodium channel was as high as 97.67% and 97.65%, respectively. The cloned sequence contains typical features of the insect sodium ion channel and contains DENS and MFM. Successfully cloned the sodium ion channel gene of Myzus persicae, laying the foundation for elucidating its molecular mechanism of target resistance to pyrethroids.

Key words: Myzus persicae, sodium channel, gene cloning, bioinformatics analysis, phylogenetic analysis

中图分类号:

S476

王颢, 段文波, 李芬, 李为争, 吴少英. 桃蚜电压门控钠离子通道基因cDNA克隆及其生物信息学分析[J]. 中国生物防治学报, 2020, 36(3): 443-451.

WANG Hao, DUAN Wenbo, LI Fen, LI Weizheng, WU Shaoying. Cloning and Bioinformatics Analysis of Voltage-gated Sodium Channel Gene of Myzus persicae[J]. Chinese Journal Of Biological Control, 2020, 36(3): 443-451.

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