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中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (6): 891-898.DOI: 10.16409/j.cnki.2095-039x.2020.06.011

• 研究论文 • 上一篇    

七星瓢虫不同虫态的低温贮藏条件

王小强1,2, 曹馨月3, 周永航1, 陈四维1, 牟方生1, 蒲德强2   

  1. 1. 四川省农业科学院经济作物育种栽培研究所, 成都 610300;
    2. 四川省农业科学院植物保护研究所, 成都 610066;
    3. 四川省农业科学院茶叶研究所, 成都 610066
  • 收稿日期:2019-03-08 发布日期:2021-01-09
  • 通讯作者: 蒲德强,副研究员,E-mail:pdqpudeqiang@163.com。
  • 作者简介:王小强,硕士,E-mail:jim9112008@163.com
  • 基金资助:
    国家重点研发计划(2018YFD0201300);四川省农业科学院青年基金(2019QNJJ-004);四川省烟草公司重点科技项目(SCYC201805)

Low Temperature Storage of Different Stages of Coccinella septempunctata

WANG Xiaoqiang1,2, CAO Xinyue3, ZHOU Yonghang1, CHEN Siwei1, MOU Fangsheng1, PU Deqiang2   

  1. 1. Industrial Crop Research Institute, Sichuan Academy of Agricultural Science, Chengdu 610300, China;
    2. Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, China;
    3. Tea Research Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, China
  • Received:2019-03-08 Published:2021-01-09

摘要: 为了探究七星瓢虫不同虫态的低温贮藏条件,本研究在不同恒定低温-时间与变温预处理后恒定低温-时间组合下分别测定了七星瓢虫卵、蛹及成虫的孵化率、羽化率及成虫存活率。结果表明,无论恒定低温或变温预处理,卵在常温下3 d均可孵化,恒温和变温的孵化率分别为72.56%和73.93%,且当保存时间不超过5 d时,卵在8℃、10℃及12℃时孵化率均在50%以上,保存时间为10 d时,卵在10℃及12℃时孵化率均在50%以上。七星瓢虫蛹在恒温和变温预处理条件下,在10℃保存3 d时均可完全羽化,且羽化率与对照组无显著差异,但当保存时间达到50 d,恒定温度在2℃、4℃、6℃和8℃下畸形率达到了100%,而其余保存天数下均未出现畸形率,当变温预处理后,仅在2℃下保存20、30和50 d分别出现100%、50%和100%的畸形率。在25℃保存时,恒温和低温预处理下的成虫保存40 d时,全部死亡,但恒温8℃下保存60 d和变温预处理10℃下保存60 d的成虫存活率分别达到60.46%和63.29%。综合分析表明,一定时间下的恒定低温和变温预处理保存均能延长七星瓢虫卵、蛹及成虫寿命,从而为七星瓢虫不同虫态商品化及其货架期的延长提供有效支持。

关键词: 七星瓢虫, 低温贮藏, 不同虫态, 存活率, 货架期

Abstract: In order to explore the suitable condition of low temperature storage of Coccinella septempunctata, the hatching rate of egg, pupal abnormal rate and adult survival rate were measured under the combination of constant temperatures and storage duration and the combination of varying temperatures and storage duration. The results showed that eggs experienced constant low temperature storage or varying temperature hatched in three days, the hatching rate was 72.56% at constant temperature and 73.93% at varying temperature. The egg hatching rate exceeded 50% when the eggs were stored for less than 5 days at 8, 10 and 12 ℃ or for less than 10 days at 10 and 12 ℃. The adult emergence rate did not differ between the pupae stored for 3 d at 10 ℃ constant temperature and varying temperature and the pupae in the control. The adult abnormal rate was 100% in the pupae stored for 50 days at the constant temperatures of 2, 4, 6 and 8 ℃ while the adult abnormal rates were 100%, 50% and 100%, respectively, in the pupae stored for 20, 30 and 50 days at the varying temperature of 2 ℃. The adult survival rate was 60.46% at the constant temperature 8 ℃ for 60 d while the survival rate was 63.29% at the constant temperature 10 ℃ for 60 d. But the adults stored for 40 days all died at constant and varying temperature of 25 ℃. Therefore, the lifetimes of egg, pupa and adult stored at the condition of low temperatures can be extended, which provides support to the commercialized production and shelf-life of different stages of C. septempunctata.

Key words: Coccinella septempunctata, low temperature store, different stage, survival rate, shelf-life

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