贮藏前补充人工饲料对七星瓢虫低温耐受力的影响

doi:10.16409/j.cnki.2095-039x.2020.05.006

摘要/Abstract

摘要： 七星瓢虫是一种重要的捕食性天敌，在生物防治中应用广泛。为了探究贮藏前补充人工饲料对该瓢虫低温耐受力的影响，本研究通过在贮藏前对新羽化成虫饲喂人工饲料（简称AD）及豌豆蚜（对照，简称AP），以6℃下不同时间后的适合度来评价两个处理的低温耐受力。结果表明：随着贮藏时间延长，AD成虫的存活率呈下降趋势，在60 d时降至80%，但AP在整个贮藏期均具有较高存活率（>95%）且AP-45显著高于AD-45；AD成虫体质量损失随贮藏时间延长也显著增加，但均低于AP；AD-60仍具较高生殖力（产卵量为46.4粒，孵化率为80.6%），与AP-60差异不显著。结果表明人工饲料可作为七星瓢虫成虫贮藏前的一种替代食物，将在进一步开发经济、高效的低温贮藏技术中发挥重要作用。

关键词: 低温贮藏, 七星瓢虫, 人工饲料, 生物防治

Abstract: Coccinella septempunctata is an important biocontrol agent and play critical roles in many biological control programs. In order to explore the effect of supplementation of artificial diet before storage on the cold tolerance of C. septempunctata, in this study, the newly emerged adults were fed with artificial diet (called AD) or pea aphid (served as control, and called AP) and then their post-storage fitness was measured after being stored at 6℃ for different periods. After that, the cold tolerance of the two treatments was evaluated. The results showed that the survival of AD adults decreased with prolonged storage and decreased to 80% at 60 days after storage, but AP adults had sustained high survival (>95%) during the whole storage periods, and survival of AP-45 was significantly higher than that of AD-45. The weight loss of AD adults was greatly decreased with prolonged storage, but was less than AP. AD-60 still had relatively high reproductive capabilities (number of oviposition was 46.4, and egg hatch rate was 80.6%), which were similar to those of AP-60. These results indicated that artificial diet could be used as an effective alternative pre-storage diet, and would play important roles in further developing cost-effective storage techniques.

Key words: cold storage, Coccinella septempunctata, artificial diet, biological control

中图分类号:

S476.2

孙元星, 郝亚楠, 李明凌. 贮藏前补充人工饲料对七星瓢虫低温耐受力的影响[J]. 中国生物防治学报, 2020, 36(5): 708-713.

SUN Yuanxing, HAO Yanan, LI Mingling. Effect of Supplementation of Artificial Diet before Storage on Cold Tolerance of Coccinella septempunctata[J]. Chinese Journal Of Biological Control, 2020, 36(5): 708-713.

参考文献

[1] Rathee M, Ram P. Impact of cold storage on the performance of entomophagous insects:an overview[J]. Phytoparasitica, 2018, 46:421-449.
[2] Costa V H D, Soares M A, Junior S L, et al. Egg storage of Podisus nigrispinus (Hemiptera:Pentatomidae) predators at low temperatures[J]. Revista Arvore, 2016, 40(5):877-884.
[3] Riddick E W, Wu Z. Potential long-term storage of the predatory mite Phytoseiulus persimilis[J]. Biocontrol, 2010, 55:639-644.
[4] Rathee M, Ram P, Mehra S. Response of Aenasius bambawalei Hayat (Hymenoptera:Encyrtidae) adults to low temperature storage in relation to key biological parameters[J]. Journal of Biological Control, 2015, 29(1):119-121.
[5] Singhamuni S A A, Hemachandra K S, Sirisena U G A I. Potential for mass rearing of the egg parasitoids, Trichogramma chilonis and Tricogramma achaeae (Hymenoptera:Trichogrammatidae) on Corcyra cephalonica eggs[J]. Tropical Agricultural Research, 2015, 27(1):1-12.
[6] 沈祖乐, 李翌菡, 周雅婷, 等. 天敌昆虫低温贮藏研究进展[J]. 热带作物学报, 2017, 2:191-197.
[7] Sun Y X, Tong C, Liu T X. Influence of larval rearing temperature on the quality of cold-stored Oomyzus sokolowskii Kurdjumov (Hymenoptera:Eulophidae)[J]. Journal of Applied Entomology, 2017, 141(3):172-180.
[8] Bowler K, Terblanche J S. Insect thermal tolerance:what is the role of ontogeny, ageing and senescence?[J]. Biological Reviews, 2008, 83(3):339-355.
[9] Jeno K, Brokordt K. Nutritional status affects the capacity of the snail Concholepas concholepas to synthesize Hsp70 when exposed to stressors associated with tidal regimes in the intertidal zone[J]. Marine Biology, 2014, 161:1039-1049.
[10] Colinet H, Boivin G. Insect parasitoids cold storage:a comprehensive review of factors of variability and consequences[J]. Biological Control, 2011, 58(2):83-95.
[11] Liu Z D, Gong P Y, Wu K J, et al. Effects of larval host plants on over-wintering preparedness and survival of the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)[J]. Journal of Insect Physiology, 2007, 53(10):1016-1026.
[12] Coudron T A, Ellersieck M R, Shelby K S. Influence of diet on long-term cold storage of the predator Podisus maculiventris (Say) (Heteroptera:Pentatomidae)[J]. Biological Control, 2007, 42(2):186-195.
[13] 程英, 郅军锐, 周宇航, 等. 非昆虫源人工饲料饲养的七星瓢虫对豆蚜的捕食功能[J]. 中国生物防治学报, 2018, 34(2):209-213.
[14] 曾凡荣. 昆虫人工饲料研究[J]. 中国生物防治学报, 2018, 34(2):184-197.
[15] Zou D Y, Wu H H, Coudron T A, et al. A meridic diet for continuous rearing of Arma chinensis (Hemiptera:Pentatomidae:Asopinae)[J]. Biological Control, 2013, 67(3):491-497.
[16] Sun Y X, Hao Y N, Riddick E W, et al. Factitious prey and artificial diets for predatory lady beetles:current situation, obstacles, and approaches for improvement:a review[J]. Biocontrol Science and Technology, 2017, 27(5):601-619.
[17] Sun Y X, Hao Y N, Liu C Z, et al. Artificial diet is fruitful pre-storage nutrition for long-term cold storage of laboratory-reared Harmonia axyridis (Pallas) adults[J]. Biological Control, 2019, 139:104075.
[18] 程英, 李忠英, 李凤良. 七星瓢虫的研究进展[J].贵州农业科学, 2006, 34(5):117-119.
[19] 肖达, 郭晓军, 张帆, 等. 环境颜色对七星瓢虫产卵的影响[J].中国生物防治学报, 2017, 33(1):44-48.
[20] 肖达, 杜晓艳, 陈旭, 等. 七星瓢虫卵的低温贮藏条件研究[J].环境昆虫学报, 2019, 41(2):288-293.
[21] Hamalainen M, Markkula M. Cool storage of Coccinella septempunctata and Adalia bipunctata (Col.:Coccinellidae) eggs for use in the biological control in greenhouses[J]. Annales Agriculturae Fenniae, 1977, 16:132-136.
[23] Hodek I, Ruzicka Z, Sehnal F. Termination of diapause by juvenoids in two species of ladybirds (Coccinellidae)[J]. Experientia, 1973, 29:1146-1147.
[23] Hamalainen M. Storing dormant Coccinella septempunctata and Adalia bipunctata (Col.:Coccinellidae) adults in the laboratory[J]. Annales Agriculturae Fenniae, 1977, 16:184-187.
[24] SunY X, Hao Y N, Liu T X. A β-carotene-amended artificial diet increases larval survival and be applicable in mass rearing of Harmonia axyridis[J]. Biological Control, 2018, 123:105-110.
[25] Ruan C C, Du W M, Wang X M, et al. Effect of long-term cold storage on the fitness of pre-wintering Harmonia axyridis (Pallas)[J]. Biocontrol, 2012, 57:95-102.
[26] Osman, M Z, Selman B J. Storage of Chrysoperla carnea Steph. (Neuroptera:Chrysopidae) eggs and pupae[J]. Journal of Applied Entomology, 1993, 115(1-5):420-424.
[27] Nedvĕd O. Comparison of cold hardiness in two ladybird beetles (Coleoptera:Coccinellidae) with contrasting hibernation behavior[J]. European Journal of Entomology, 1993, 90:465-470.
[28] 邬梦静, 徐青叶, 刘雅, 等. 异色瓢虫低温胁迫下过冷却点变化及抗寒基因表达分析[J]. 中国农业科学, 2016, 49(4):677-685.
[29] 孙毅, 万方浩, 姬金红, 等. 利用人工卵赤眼蜂蛹规模化饲养七星瓢虫的可行性研究[J]. 植物保护学报, 2001, 28(2):139-145.