猪毛蒿精油及其活性成分萜品-4-醇对黄曲霉的抑制作用研究

doi:10.16409/j.cnki.2095-039x.2021.02.013

摘要/Abstract

摘要： 为了探究猪毛蒿Artemisia scoparia精油及其活性成分萜品—4—醇对黄曲霉Aspergillus flavus的抗菌活性及其在花生贮藏保鲜中的应用前景，通过气相色谱—质谱联用（Gas Chromatography—Mass Spectrometer， GC—MS）技术对猪毛蒿精油进行成分分析；采取熏蒸法检测猪毛蒿精油及萜品—4—醇对黄曲霉菌丝生长，孢子萌发和花生体内霉菌数的影响；通过扫描电镜观察猪毛蒿精油和萜品—4—醇对黄曲霉显微结构的影响。结果显示猪毛蒿精油由24种化合物组成，其主要成分为桉叶油醇（43.8%），萜品—4—醇在猪毛蒿精油中的含量为2.81%；猪毛蒿精油和萜品—4—醇可显著抑制黄曲霉菌丝的生长，并导致菌丝皱缩、扭曲和凹陷；体内抑菌试验发现，猪毛蒿精油和萜品—4—醇能显著降低花生体内霉菌数。

关键词: 猪毛蒿精油, 成分分析, 萜品-4-醇, 黄曲霉, 抑菌活性

Abstract: In order to explore the antibacterial activity of essential oil from Artemisia scoparia and its active ingredient terpinen−4−ol against Aspergillus flavus and its application prospects in the storage and preservation of peanuts, the composition of essential oil from A. scoparia were detected by gas-phase mass spectrometry (GC−MS); Fumigation method was used to detect the effects of essential oil from A. scoparia and terpinen−4−ol on A. flavus mycelial growth and mold counts in peanuts; Scanning electron microscope (SEM) was used to observe the effects of essential oil from A. scoparia and terpinen−4−ol on A. flavus influence of microstructure. The results showed that essential oil from A. scoparia contains 24 compounds, the main component of which is eucalyptol (43.8%), the content of terpinen−4−ol in A. scoparia essential oil was 2.81%; Essential oil from A. scoparia and terpinen−4−ol can significantly inhibit the growth of A. flavus hyphae, and cause the hyphae to shrink, twist and dent; In vivo antibacterial experiments found that essential oil from A. scoparia and terpinen−4−ol can significantly reduce the number of mold in peanuts.

Key words: essential oil from Artemisia scoparia, component analysis, terpine-4-ol, Aspergillus flavus, antifungal activity

中图分类号:

S476

令利军, 赵云花, 马稳霞, 冯升来, 杨彩云, 涂艺馨, 张继. 猪毛蒿精油及其活性成分萜品-4-醇对黄曲霉的抑制作用研究[J]. 中国生物防治学报, 2021, 37(3): 584-591.

LING Lijun, ZHAO Yunhua, MA Wenxia, FENG Shenglai, YANG Caiyun, TU Yixin, ZHANG Ji. Study on the Inhibitory Effect of Essential Oil from Artemisia scoparia and Its Active Ingredient Terpine-4-ol on Aspergillus flavus[J]. Chinese Journal of Biological Control, 2021, 37(3): 584-591.

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