解淀粉芽胞杆菌XJ5挥发性物质抑菌活性及对苹果褐腐病防效测定

doi:10.16409/j.cnki.2095-039x.2020.04.018

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (4): 575-580.DOI: 10.16409/j.cnki.2095-039x.2020.04.018

解淀粉芽胞杆菌XJ5挥发性物质抑菌活性及对苹果褐腐病防效测定

郭洁心, 张育铭, 朱洪磊, 任璐, 张宝俊

山西农业大学植物保护学院, 太谷 030801

收稿日期:2020-02-05 出版日期:2020-08-08 发布日期:2020-08-07
通讯作者: 张宝俊,副教授,E-mail:zhangbj992@163.com。
作者简介:郭洁心,女,硕士研究生,E-mail:15234483340@163.com
基金资助:
山西省科技基础条件平台项目（201605D121024）；山西省应用基础研究计划（201801D121244）

Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena

GUO Jiexin, ZHANG Yuming, ZHU Honglei, REN Lu, ZHANG Baojun

College of Agronomy, Shanxi Agricultural University, Taigu 030801, China

Received:2020-02-05 Online:2020-08-08 Published:2020-08-07

摘要/Abstract

摘要： 解淀粉芽胞杆菌Bacillus amyloliquefaciens XJ5挥发性物质对苹果褐腐病菌Monilinia fructigena等多种病原真菌具有良好的拮抗活性。通过固相微萃取-气相色谱质谱联用仪（Solid-phase microextraction-Gas chromatography mass spectrometry，SPME-GC-MS）对菌株XJ5产生的挥发性物质进行检测，利用平板对扣法测定挥发性化合物的抑菌活性，并离体接种评价菌株XJ5挥发性物质对苹果褐腐病的防效。结果表明，XJ5菌株挥发性物质对9种植物病原真菌的抑菌率在47.9%~84.8%，其中对苹果褐腐病菌的抑菌率最高为84.8%。SPME-GC-MS检测及抑菌活性测定表明，菌株XJ5分泌的主要挥发性物质为十二醛（Dodecanal），具有抑菌活性的挥发性物质主要为2-壬醇（2-Nonanol）、2-乙基己醇（2-Ethyl-1-hexanol）和2-十一醇（2-Undecanol）。其中2-壬醇抑制苹果褐腐病菌的EC₅₀为3.43 μg/mL，且XJ5菌液离体熏蒸与2-壬醇离体熏蒸均可有效抑制苹果褐腐病菌丝生长及病斑扩展，其对苹果褐腐病的离体防效分别在14.4%~71.2%和66.4%~97.6%，与对照差异显著。

关键词: 解淀粉芽胞杆菌, 挥发性物质, SPME-GC-MS, 抑菌活性, 离体防效

Abstract: Volatile organic compounds (VOCs) of Bacillus amyloliquefaciens XJ5 have good antifungal activity against various pathogenic fungi such as Monilinia fructigena. The VOCs produced by strain XJ5 were detected by SPME-GC-MS, their antifungal activities were determined by two-sealed-base-plates method, and the control effects against M. fructigena were evaluated in vitro. The results indicated that inhibition rate of XJ5 VOCs against nine pathogenic fungi were ranged between 47.9% and 84.8%, and the highest inhibition rate against M. fructigena was 84.8%. SPME-GC-MS analysis and antifungal activity determination showed that the major VOCs produced by strain XJ5 were Dodecanal, and the major VOCs with antifungal activity were 2-Nonanol, 2-Ethyl-1-hexanol and 2-Undecanol. The EC₅₀ value of 2-Nonanol inhibiting M. fructigena was 3.43 μg/mL. In vitro fumigation with XJ5 bacteria suspension and 2-Nonanol can effectively inhibited the mycelial growth of M. fructigena and the lesion development. The fumigation control efficacies of XJ5 bacteria suspension and 2-Nonanol against M. fructigena were 14.4%~71.2% and 66.4%~97.6%, respectively, significantly different from those of the control.

Key words: Bacillus amyloliquefaciens, volatile organic compounds, SPME-GC-MS, antifungal activity, control effectin in vitro

中图分类号:

S476

郭洁心, 张育铭, 朱洪磊, 任璐, 张宝俊. 解淀粉芽胞杆菌XJ5挥发性物质抑菌活性及对苹果褐腐病防效测定[J]. 中国生物防治学报, 2020, 36(4): 575-580.

GUO Jiexin, ZHANG Yuming, ZHU Honglei, REN Lu, ZHANG Baojun. Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena[J]. Chinese Journal Of Biological Control, 2020, 36(4): 575-580.

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解淀粉芽胞杆菌XJ5挥发性物质抑菌活性及对苹果褐腐病防效测定

Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena

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