无毒稻瘟菌株AM16诱导水稻稻瘟病抗性研究

doi:10.16409/j.cnki.2095-039x.2023.02.014

摘要/Abstract

摘要： 利用病原菌无毒或弱毒菌株诱导植物抗病性是植物病害生物防控的重要研究方向。前期研究中，鉴定了一株对所有测试水稻都不致病的无毒稻瘟菌株AM16。本研究用AM16菌株与致病稻瘟菌株对峙培养，结果表明AM16菌株对致病稻瘟菌株生长无拮抗作用。本研究利用AM16培养液喷施水稻叶片诱导稻瘟病抗性，初步研究结果表明，AM16培养液增强了幼苗期水稻植株抗瘟性，且水稻植株在喷施摇瓶培养5 d以内的AM16培养液48-72 h稻瘟病抗性较稳定地维持。温室和大田试验结果表明，喷施AM16培养液增强分蘖期和抽穗期水稻植株抗瘟性且防效均在40%以上；水稻植株体内防御相关酶PPO、PAL、SOD、POD、APX和CAT活性不同程度上升，SA和JA通路关键基因（OsPAL1、OsNPR1和OsAOS2）和防御相关基因（OsChit1、OsPR10b、OsPR1a和OsPR1b）表达上调。该研究为AM16菌株在稻瘟病生物防治中的开发利用奠定了重要基础。

关键词: 水稻, 稻瘟病抗性, 无毒稻瘟菌株AM16, 诱导抗性, 生物防治

Abstract: It is an important research direction in biocontrol of plant diseases to induce disease resistance of plant by avirulent or attenuated strains. In previous study, we identified an avirulent Magnaporthe oryzae strain AM16 which was avirulent to all tested rice varieties. In this study, AM16 strain was cultured with pathogenic rice blast strain, and it had no antagonistic effect on the growth of pathogenic strain. When rice leaves were sprayed with AM16 culture medium, the medium enhanced the blast resistance of rice plants at seedling stage, and the resistance was maintained stably 48-72 h after spraying AM16 culture medium within 5 days of shake flask culture. Greenhouse and field experiments showed that spraying AM16 culture solution enhanced the blast resistance of rice plants at tillering stage and heading stage, and the control efficacy was more than 40%. The activity of defense-related enzymes (PPO, PAL, SOD, POD, APX and CAT), the expression level of key genes in SA- and JA- signaling pathways (OsPAL1, OsNPR1 and OsAOS2), and defense-related genes (OsChit1, OsPR10b, OsPR1a and OsPR1b) were up-regulated in rice plants treated with AM16 culture solution. This study laid an important foundation for further development and utilization of AM16 strains in biological control of rice blast.

Key words: rice, rice blast resistance, avirulent rice blast strain AM16, induced resistance, biocontrol

中图分类号:

S476

焦泽宇, 陈亚莉, 王波, 胡吉会, 曾千春, 罗琼. 无毒稻瘟菌株AM16诱导水稻稻瘟病抗性研究[J]. 中国生物防治学报, 2023, 39(2): 366-379.

JIAO Zeyu, CHEN Yali, WANG Bo, HU Jihui, ZENG Qianchun, LUO Qiong. Study on Blast Resistance of Rice Induced by Avirulent Magnaporthe Oryzae Strain AM16[J]. Chinese Journal of Biological Control, 2023, 39(2): 366-379.

参考文献

[1] 张启发. 绿色超级稻培育的设想[J]. 分子植物育种, 2005, 3(5):601-602.
[2] Liu W, Liu J, Triplett L, et al. Novel insights into rice innate immunity against bacterial and fungal pathogens[J]. Annual Review Phytopathology, 2014, 52(1):213-241.
[3] Meng Q, Guptar R, Min C W, et al. Proteomics of rice-Magnaporthe oryzae Interaction:What have we learned so far?[J]. Frontiers in Plant Science, 2019, 10(1383).
[4] Skamnioti P, Gurr S J. Against the grain:safeguarding rice from rice blast disease[J]. Trends in Biotechnology, 2009, 27(3):141-150.
[5] Talbot N J. On the trail of a cereal killer:Exploring the biology of Magnaporthe grisea[J]. Annual Review of Microbiology, 2003, 57(1):177-202.
[6] Wilson R A, Talbot N J. Under pressure:investigating the biology of plant infection by Magnaporthe oryzae[J]. Nature Review Microbiology, 2009, 7(3):185-195.
[7] Groth D E, Bond J A. Effects of cultivars and fungicides on rice sheath blight, yield, and quality[J]. Plant Disease, 2007, 91(12):1647-1650.
[8] 殷得所, 夏明元, 李进波, 等. 抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用[J]. 中国水稻科学, 2011, 25(1):25-30.
[9] Onaga G, Wydra KD, Koopmann B, et al. Elevated temperature increases in planta expression levels of virulence related genes in Magnaporthe oryzae and compromises resistance in Oryza sativa cv. nipponbare[J]. Functional Plant Biology. 2017, 44(3):358-371.
[10] 沙月霞, 王琦, 李燕. 稻瘟病生防芽胞杆菌的筛选及防治效果[J]. 中国生物防治学报, 2016, 32(4):474-484.
[11] 陈捷. 木霉菌诱导植物抗病性研究新进展[J]. 中国生物防治学报, 2015, 31(5):733-741.
[12] Manandhar H K, Lyngs Jorgensen H J, Mathur S B, et al. Suppression of rice blast by preinoculation with avirulent Pyricularia oryzae and the nonrice pathogen Bipolaris sorokiniana[J]. Phytopathology, 1998, 88(7):735-739.
[13] Omoboye O O, Oni F E, Batool H, et al. Pseudomonas cyclic lipopeptides suppress the rice blast fungus Magnaporthe oryzae by induced resistance and direct antagonism[J]. Frontiers in Plant Science, 2019, 10:901.
[14] 范志金, 刘秀封, 刘凤丽, 等. 植物抗病激活剂诱导植物抗病性的研究[J]. 植物保护学报, 2005(1):87-92.
[15] 陈桂华, 柏连阳, 肖艳松. 水稻稻瘟病诱导抗病性的研究进展[J]. 中国农学通报, 2005, 21(6):326-330.
[16] Wu Z, Wang G, Zhang B, et al. Mechanism of plant defense against rice blast induced by probenazole[J]. Metabolites, 2021, 11(4):246.
[17] Yoshioka K, Nakashita H, Klessig DF, et al. Probenazole induces systemic acquired resistance in Arabidopsis with a novel type of action[J]. Plant Journal, 2001, 25(2):147-157.
[18] 戴晗. 利用稻瘟病菌致病缺陷突变体诱导水稻抗病性[D]. 浙江大学, 2020.
[19] 范静华, 周慧萍, 王洪珍, 等. 稻瘟菌诱导的广谱抗性[J]. 石河子大学学报(自然科学版), 2004(S1):30.
[20] Iwai, T, Seo, et al. Probenazole-induced accumulation of salicylic acid confers resistance to Magnaporthe grisea in adult rice plants[J]. Plant & Cell Physiology, 2007, 48:915-924.
[21] Dangol S, Chen Y, Hwang B K, et al. Iron-and reactive oxygen species-dependent ferroptotic cell death in rice-Magnaporthe oryzae interactions[J]. Plant Cell, 2019, 31(1):189
[22] 吴成龙, 靳学慧, 张亚玲, 等. 不同抗性水稻品种幼苗接种稻瘟病菌弱毒菌株后的生化反应[J]. 黑龙江八一农垦大学学报, 2008, 20(2):27-30.
[23] Wang X, Xiao Q, Xu Y, et al. Genome sequence resource of an avirulent Magnaporthe oryzae field strain AM16[J]. Plant Disease, 2022, 106(8):2243-2246.
[24] 凌忠专, 蒋琬如, 王久林, 等. 水稻品种丽江新团黑谷普感特性的研究和利用[J]. 中国农业科学, 2001, 34(1):116.
[25] 张袆颖, 洪汝科, 张锦文, 等. 花药培养获得优质抗稻瘟病水稻[J]. 西南农业学报, 2008, 21(1):75-79.
[26] 张锦文, 洪汝科,范静华, 等. 一份云南地方稻广谱持久抗稻瘟病初步分析[J]. 西南农业学报, 2011, 24(4):1323-1326.
[27] 李开斌, 张天春, 阮文忠, 等. 超级粳稻楚粳27号的选育及应用[J]. 中国稻米, 2012, 18(5):71-72.
[28] Arora R, Wisniewski M E. Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica[L.] Batsch). II. A 60-kilodalton bark protein in cold-acclimated tissues of peach is heat stable and related to the dehydrin family of proteins[J]. Plant Physiology, 1994, 105(1):95-101.
[29] Zhou W, Zhao D, Lin X. Effects of waterlogging on nitrogen accumulation and alleviation of waterlogging damage by application of nitrogen fertilizer and mixtalol in winter rape (Brassica napus L.)[J]. Journal of Plant Growth Regulation, 1997, 16(1):47-53.
[30] Muñoz-Muñoz J L, García-Molina F, García-Ruiz P A, et al. Enzymatic and chemical oxidation of trihydroxylated phenols[J]. Food Chemistry, 2008, 113(2):435-444.
[31] 裴斌, 张光灿, 张淑勇, 等. 土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J]. 生态学报, 2013, 33(5):1386-1396.
[32] 孙云, 江春柳, 赖钟雄, 等. 茶树鲜叶抗坏血酸过氧化物酶活性的变化规律及测定方法[J]. 热带作物学报, 2008, 29(5):562-566.
[33] 赵飞, 李园莉, 杨亦扬, 等. 远红外辐射对萎凋叶氧化酶活性及红茶品质的影响[J]. 中国农学通报, 2021, 37(7):119-125.
[34] Jing L. On the change of enzyme activites of cucumber leaf infected by Pseudoperonospora cubensis (berk.et ctrt) rosws[J]. Acta Phytopathologica Sinica, 1991.
[35] 霍建玲, 邢雪莹, 杨雪, 等. 干旱对黑龙江省大豆品种农艺性状的影响[J]. 分子植物育种, 2018, 16(5):1668-1676.
[36] Nzojiyobiri Jean-Berchmans, 徐同, 宋凤鸣, 等. 哈茨木霉NF9菌株对水稻的诱导抗病性[J]. 中国生物防治, 2003(3):111-114.
[37] Fridovich I. The biology of oxygen radicals[J]. Science, 1978, 201(4359):875-880.
[38] Chen Z, Silva H, Klessig D F. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid[J]. Science, 1993, 262:1882-1886.
[39] 葛秀春, 宋凤鸣. 水稻-稻瘟病菌互作中抗氧化物质含量及相关酶活性的变化[J]. 浙江大学学报(农业与生命科学版), 1998, 24(4):387-391.
[40] Wang G, Ding X, Yuan M, et al. Dual function of rice OsDR8 gene in disease resistance and thiamine accumulation[J]. Plant Molecular Biology, 2006, 60(3):437-449.
[41] Kim E J, Hong W J, Tun W, et al. Interaction of OsRopGEF3 Protein With OsRac3 to Regulate Root Hair Elongation and Reactive Oxygen Species Formation in Rice (Oryza sativa)[J]. Frontiers in Plant Science, 2021, 12:661352.
[42] 王瑞霞, 王振中, 纪春艳, 等. 水杨酸诱导水稻抗菌物质对稻瘟病菌的抑制作用[J]. 华中农业大学学报, 2011, 30(2):193-196.
[43] 李艳, 高宝嘉, 陈连涛. 植物诱导抗性的分子机制研究进展[J]. 河北果树研究, 2013, 28(4):427-430.
[44] Yin L, Gan X, Shi J, et al. Induced resistance mechanism of novel curcumin analogs bearing a quinazoline moiety to plant virus[J]. International Journal of Molecular Sciences, 2018, 19(12):4065.
[45] Paudel S, Lin P A, Foolad M R, et al. Induced plant defenses against herbivory in cultivated and wild tomato[J]. Journal of Chemical Ecology, 2019, 45(8):693-707.
[46] 邹志燕, 王振中. 茉莉酸诱导水稻幼苗对稻瘟病抗性作用研究[J]. 植物病理学报, 2006, 36(5):432-438.
[47] 刘晓光, 高克祥, 康振生, 等. 生防菌诱导植物系统抗性及其生化和细胞学机制[J]. 应用生态学报, 2007, 18(8):1861-1867.
[48] 刘鹤, 陈建光, 姜军, 等. 生防细菌SYL-3调控叶际微生物群落防治烟草病害研究[C]//植物病理科技创新与绿色防控-中国植物病理学会2021年学术年会, 中国贵州贵阳, 2021, 594.
[49] Slaughter A, Daniel X, Flors V, et al. Descendants of primed Arabidopsis plants exhibit resistance to biotic stress[J]. Plant Physiology, 2012, 158(2):835-843.
[50] Ramírez-Carrasco G, Martínez-Aguilar K, Alvarez-Venegas R. Transgenerational defense priming for crop protection against plant pathogens:a hypothesis[J]. Frontiers in Plant Science, 2017, 8:696.