一株拮抗菜豆普通细菌性疫病链霉菌的筛选、鉴定及防效研究

doi:10.16409/j.cnki.2095-039x.2023.02.060

摘要/Abstract

摘要： 为寻找有潜力的生防菌株，本研究通过共培养初筛和牛津杯复筛从土壤中分离筛选获得123株放线菌。最终获得1株对菜豆普通细菌性疫病病菌Xanthomonas axonopodis pv. phaseoli（Xap）抑制能力最强的菌株Sa-4，发酵液抑菌圈直径为（31.62±3.23）mm。根据显微形态观察、生理生化特性测定并结合16S rDNA序列分析，鉴定菌株Sa-4为阿维丁链霉菌Streptomyces avidinii。抗菌谱测定结果表明，菌株Sa-4发酵液对多种植物病原菌均具有较强的拮抗作用。稳定性试验结果表明，菌株Sa-4的发酵液具有较好的光稳定性和热稳定性。抑菌机理研究结果显示，菌株Sa-4发酵液对Xap的基因组DNA无明显作用；对Xap蛋白质合成有一定的影响；傅立叶变换红外光谱结果显示，菌株Sa-4发酵液会导致Xap细胞膜表面的物质发生改变；电镜观察结果显示Xap细胞出现皱缩、凹陷等现象。防效结果表明，菌株Sa-4发酵液对菜豆普通细菌性疫病的相对防效可达68.69%。本研究结果为阿维丁链霉菌菌株Sa-4在菜豆普通细菌性疫病的生物防治产品开发提供基础参考数据。

关键词: 阿维丁链霉菌, 菜豆普通细菌性疫病, 筛选鉴定, 生物防治

Abstract: In order to find potential biocontrol strains, 123 strains of actinomycetes were isolated and screened from soil by preliminary co-culture screening and Oxford cup re-screening. Ultimately, the strain Sa-4 was obtained, which had the strongest inhibitory ability against the pathogen of common bacterial blight of beans, Xanthomonas axonopodis pv. phaseoli (Xap). The antibacterial ring diameter of its fermentation broth was (31.62±3.23) mm. Based on microscopic observation, physiological and biochemical characteristics determination combined with 16S rDNA sequence analysis, the strain Sa-4 was identified as Streptomyces avidinii. The results of antibacterial spectrum determination showed that the fermentation broth of strain Sa-4 had strong antagonistic effects on various plant pathogens. The results of stability test showed that the fermentation broth of strain Sa-4 had good light stability and thermal stability. The results of antibacterial mechanism study showed that the fermentation broth of strain Sa-4 had no significant effect on the genomic DNA of Xap but had a certain influence on the protein synthesis of Xap. Fourier-transform infrared spectroscopy showed that the fermentation broth of strain Sa-4 would cause changes in the substances on the surface of Xap cell membrane, while results of electron microscopy showed that Xap cells were shrinked and depressed. The relative control efficacy of strain Sa-4 fermentation broth against common bacterial blight of beans could reach 68.69% by pot experiment. The results of this study provided basic reference data for the biocontrol products development of Streptomyces avidinii strain Sa-4 in common bacterial blight of beans.

Key words: Streptomyces avidinii, common bacterial blight of beans, screening and identification, biocontrol

中图分类号:

S476

袁歆瑜, 邵嘉朱, 宋振, 廖鑫琳, 蒋冬花. 一株拮抗菜豆普通细菌性疫病链霉菌的筛选、鉴定及防效研究[J]. 中国生物防治学报, 2023, 39(6): 1408-1417.

YUAN Xinyu, SHAO Jiazhu, SONG Zhen, LIAO Xinlin, JIANG Donghua. Screening, Identification and Control Efficacy Study of a Streptomyces Strain Antagonistic to Common Bacterial Blight of Beans[J]. Chinese Journal of Biological Control, 2023, 39(6): 1408-1417.

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