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Clone, Expression and CDA Activity of Chitin Deacetylase 1 from Hyphantria cunea (Drury)

YAN Xiaoping1, ZHAO Dan1, SUN Xiaotong1, GUO Wei1,2, LI Shaoya1, LI Jing2   

  1. 1. College of Life Sciences, Agricultural University of Hebei/Crop Diseases and Pests Biological Control Engineering Technology Center of Hebei, Baoding 071000, China;2. Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China
  • Received:2014-09-29 Revised:1900-01-01 Online:2015-06-08 Published:2015-06-08

Abstract: HcCDA1 cDNA sequence was obtained by PCR and rapid amplification of cDNA ends (RACE). HcCDA1 was expressed in Escherichia coli BL21 and Pichia pastoris GS115, respectively. The HcCDA1 was identified to be a 61.8 kD protein in E.coli BL21 and 80 kD protein in P. pastoris GS115 by Western blot analysis. The CDA activity of recombinant HcCDA1 expressed in P. pastoris GS115 was 1.685 U/mL. The expression of recombinant HcCDA1 will facilitate further investigation of physiological functions of HcCDA1 protein.

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