Abstract: Six pathogenic fungi were isolated from naturally infected plant of Digitaria sanguinalis. They were identified as Curvularia intermedia, C. lunata, Helminthosporium nodulosum, Piricularia grisea, Altarnaria alternata and Fusarium roseum. Among them, C. lunata and H. nodulosum infected corn and P. grisea infected rice slightly. Under the condition of 48h dew period, A. alternata and F. roseum did not cause death of D. sanguinalis seedlings at the 1-3 leaf stage, but C. intermedia provided good control (90%-100% in greenhouse and 75% in field) of the weed at the 1-3.5 leaf stage. Initial host range test were done with C. intermedia. The pathogen did not infect rice, corn, soybean, cotton, wheat, sunflower, peanut, and lawn. C. intermedia may be a potential agent as bioherbicide to control crabgrass.