Abstract: We reared oriental migratory locusts, Locusta migratoria manilensis (Meyen) in the laboratory from egg stage until egg-laying female adults, at six different degree-days (544.3, 662.2, 678.8, 760.2, 840.0 and 843.5 heat units), to reveal the influence of degree-days (DD) on diapause rate of overwintering locust eggs. Results showed that the diapause rate of eggs at the six treatments was 73.1%, 58.6%, 48.6%, 12.1%, 0 and 0, respectively. This indicated there were only parts of overwintering locust eggs could enter diapause during winter. The relationship between diapause rate of eggs (D) and DD of pre-overwintering period (Y) was D=-0.719Y+82.75, R²=0.774. When Y≥115.1 DD, all overwintering locust eggs would hatch. We further studied the sensitive temperature on diapause rate of eggs at the six treatments, results showed that DD based on temperature over than 27 ℃ (E) had the significant effect on diapause rate of eggs (D), the relationship was D=-0.058E+0.732, R²=0.967. The diapause rate of eggs (D) would decrease with negative DD (X) arising after eggs entering diapause, which was D=-0.155 ln(|X|)+1.136, R²=0.970. Our results reveal the relationship between DD and diapause rate of eggs at different overwintering stages, which would aid in oriental migratory locusts forecasting and prediction.