Colonization of Pseudomonas fluorescens PEF-5#18 to Control Fusarium Wilt Disease on Tomato

doi:10.16409/j.cnki.2095-039x.2017.05.012

Abstract

Abstract: In order to study the colonization in rhizosphere soil as well as root-stem inside, plasmid pEGFP20 expressing green fluorescens protien was transferred into wild type Pseudomonas fluorescens PEF-5#18 with great effect against Fusarium wilt disease on tomato by electrophoretic transfer method. The results indicated that both wild type and marked P. fluorescens PEF-5#18 bio-control treatments could remarkably control Fusarium wilt disease on tomato with the efficiency of 76.92% and increase plant fresh (194.10%) or dry weight (564.71%) after 25 days, if compared with Forl positive treatment. Inoculated P. fluorescens PEF-5#18 could colonize in rhizosphere soil and extend into tomato root-stem inside, and the distribution amounts ranked as:rhizosphere soil (1.05×10⁶ CFU/g) > root (6.75×10⁴ CFU/g) > stem (1.25×10² CFU/g). Compared with pathogen positive control, the pathogen population was remarkably reduced in rhizosphere soil and root-stem inside, the cultivable bacteria population (increased 8.42% inside root and 14.78% inside stem) and pH value (increased 1.61%) were remarkably increased. Confocal microscopy observation demonstrated that P. fluorescens PEF-5#18 existed on root surface, root xylem, root endodermis cells, root intercellular space, stem vascular tissue cells and the cells around.

Key words: Pseudomonas fluorescens, tomato wilt, marker, colonization.

CLC Number:

S476

ZHANG Liang, SHENG Hao, YUAN Hong, ZHAO Lanfeng, LI Huaxing. Colonization of Pseudomonas fluorescens PEF-5#18 to Control Fusarium Wilt Disease on Tomato[J]. journal1, 2017, 33(5): 658-666.

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