Effect of Quorum Sensing Signal Substance on Endogenous Colonization of Burkholderia pyrrocinia JK-SH007 in Poplar Seedlings

doi:10.16409/j.cnki.2095-039x.2019.06.023

Abstract

Abstract: In order to clarify whether the quorum sensing of Burkholderia pyrrocinia JK-SH007 with high biocontrol activity against poplar cancer is related to its endogenous colonization, the type of quorum sensing signal substance of JK-SH007 strain was determined by the quorum sensing indicator Chromobacterium violaceum CV026 and liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS). The effects of quorum sensing signal substance on the biofilm and endogenous colonization of JK-SH007 strain in poplar seedlings were studied by crystal violet staining, colonization recovery and GFP labeling. The results showed that the quorum sensing signal substance produced by JK-SH007 strain was N-octanoyl-DL-homoserine lactone (C₈-HSL) containing acyl side chain of 8 carbon atoms. The addition of quorum sensing signal substance C₈-HSL had a certain effect on the biofilm of the strain and the colonization ability of the strain in poplar seedlings, showing promotion at low concentrations and inhibition at higher concentrations. The addition of C₈-HSL was significantly correlated with the OD value and the amount of bacteria in the strain JK-SH007 biofilm, which were 0.923 and 0.756, respectively. When 1.0% (volume fraction) of C₈-HSL was added (final concentration reached 5×10^-8 g/L), the amount of JK-SH007 biofilm formation peaked. Fluorescence microscopy revealed that when 100 μL of C₈-HSL were added, the number of colonized GFP fluorescently labeled strain JK-SH007 in roots and stems of poplar tissue culture was significantly higher than that of CK. The above results indicate that the quorum sensing of JK-SH007 strain is closely related to its endogenous colonization ability in poplar seedlings. The quorum sensing signal substance C₈-HSL can enhance the endogenous colonization effect of strain JK-SH007 in poplar seedlings, and also indicates that the substance is beneficial to the biocontrol effect of the strain.

Key words: Burkholderia pyrrocinia, poplar canker disease, quorum sensing, N-acyl-homoserine lactones, endogenous colonization

CLC Number:

S476

YANG Xin, ZHANG Pengfei, BAI Bianxia, YE Jianren, REN Jiahong. Effect of Quorum Sensing Signal Substance on Endogenous Colonization of Burkholderia pyrrocinia JK-SH007 in Poplar Seedlings[J]. Chinese Journal Of Biological Control, 2019, 35(6): 949-957.

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