Sensitivity Evaluation of Coniothyrium minitans to Rapeseed Slow-released Special Formula Fertilizer

doi:10.16409/j.cnki.2095-039x.2020.03.025

Abstract

Abstract: Sclerotinia is an important disease in rapeseed (Brassica napus) production. Coniothyrium minitans is a mycoparasite of the plant-pathogenic fungus Sclerotinia sclerotiorum, which has important biocontrol potential in the prevention and treatment of sclerotinia stem rot. In order to clarify the feasibility of the mixture of C. minitans and rapeseed slow-released special formula fertilizer (RSF), the sensitivity of C. minitans to RSF was evaluated in this paper. The results showed that the low concentration of RSF (1.5 mg/mL and 7.5 mg/mL) had no significant effect on the growth, colony morphology, branch pattern and conidia germination of C. minitans, while high concentration of RSF had some effects on the growth and conidia germination of C. minitans. Under the condition of saturated concentration of RSF (560 mg/mL), the germination rate of C. minitans conidia was only 0.83% at 24 hours post inoculation (hpi) on culture medium, however, the germination rate reached to 95% at 96 hpi. Furthermore, the fertilizer had no obvious effect on conidiation and parasitic activity to sclerotia of S. sclerotiorum. In the presence of RSF, ZS-1 was still capable of colonizing sclerotia and the sclerotia rot index exceeded 50 after 30 days. Taken together, our results showed that C. minitans can be mixed with rapeseed slow-released special formula fertilizer in field production.

Key words: Coniothyrium minitans, Sclerotinia sclerotiorum, rapeseed sclerotinia rot, rapeseed slow-released special formula fertilizer, sensitivity

CLC Number:

S476

LIU Xiaofan, XIE Jiatao, FU Yanping, JIANG Daohong, CHENG Jiasen. Sensitivity Evaluation of Coniothyrium minitans to Rapeseed Slow-released Special Formula Fertilizer[J]. Chinese Journal Of Biological Control, 2020, 36(3): 465-471.

References

[1] Bolton M D, Thomma B P, Nelson B D. Sclerotinia sclerotiorum, (Lib) de Bary:biology and molecular traits of a cosmopolitan pathogen[J]. Molecular Plant Pathology, 2006, 7(1):1-16.
[2] Boland G J, Hall R. Index of plant hosts of Sclerotinia sclerotiorum[J]. Canadian Journal Plant Pathology, 1994, 16:93-108.
[3] Wang Y, Sun Y, Zhang Y, et al. Sensitivity and biochemical characteristics of Sclerotinia sclerotiorum to propamidine[J]. Pesticide Biochemistry and Physiology, 2016, 135:82.
[4] Abawi G S. Epidemiology of diseases caused by Sclerotinia species[J]. Phytopathology, 1979, 69(8):899.
[5] Li G Q, Huang H J, Miao H J, et al. Biological control of Sclerotinia diseases of rapeseed by aerial applications of the mycoparasite Coniothyrium minitans[J]. European Journal of Plant Pathology, 2006, 114(4):345-355.
[6] 羊国根, 程家森. 核盘菌致病机理研究进展[J]. 生物技术通报, 2018, 309(4):15-21.
[7] Xu D, Pan Y, Zhang H, et al. Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China[J]. Genetics and Molecular Research, 2015, 14(4):16627.
[8] Adams P B, Ayers W A. Ecology of Sclerotinia species[J]. Phytopathology, 1979, 69(8):896.
[9] Clarkson J P, Phelps K, Whipps J M, et al. Forecasting Sclerotinia disease on lettuce:A predictive model for carpogenic germination of Sclerotinia sclerotiorum sclerotia[J]. Phytopathology, 2007, 97(5):621-631.
[10] 游景茂, 谢甲涛, 付艳苹, 等. 除草剂精禾草克和乙草胺对油菜菌核病生防菌盾壳霉生长和寄生的影响[J]. 中国生物防治, 2010, 26(3):340-346.
[11] Campbell W A. A new species of Coniothyrium parasitic on sclerotia[J]. Mycologia, 1947, 39(2):190-195.
[12] Budge S P, Whipps J M. Glasshouse trials of Coniothyrium minitans and Trichoderma species for the biological control of Sclerotinia sclerotiorum in celery and lettuce[J]. Plant Pathology, 1991, 40:59-66.
[13] Jones E E, Rabeendran N, Stewart A. Biocontrol of Sclerotinia sclerotiorum infection of cabbage by Coniothyrium minitans and Trichoderma spp.[J]. Biocontrol Science and Technology, 2014, 24(12):20.
[14] 强翠翠, 程家森, 谢甲涛, 等. 盾壳霉对2种除草剂的敏感性评价[J]. 华中农业大学学报(自然科学版), 2018, 37(5):25-31.
[15] Budge S P, McQuilken M P, Fenlon J S, et al. Use of Coniothyrium minitans and Gliocladium virens for biological control of Sclerotinia sclerotiorum in glasshouse lettuce[J]. Biological Control, 1995, 5(4):513-522.
[16] Elsheshtawi M, Elkhaky M T, Sayed S R, et al. Integrated control of white rot disease on beans caused by Sclerotinia sclerotiorum using contans and reduced fungicides application[J]. Saudi Journal of Biological Sciences, 2016, 24(2):405-409.
[17] 周鹂, 鲁剑巍, 刘涛, 等. 油菜长效专用配方肥施用效果研究[J]. 中国土壤与肥料, 2015(4):71-75.
[18] Jiang D H, Li G Q, Yi X H, et al. Characterization of the sclerotial parasite Coniothyrium minitansⅡ.comparison of the cultural characteristics and the abilities to parasitize the sclerotia of Sclerotinia sclerotiorum[J]. Journal of Huazhong Agricultural University, 1996, 15(3):229-232.
[19] 杨龙, 罗倩, 李国庆, 等. 盾壳霉与硼肥的相容性及其混用对核盘菌菌核萌发的控制[C]//中国植物病理学会2011年学术年会论文集, 2011.
[20] Yang L, Li G, Zhang J, et al. Compatibility of Coniothyrium minitans with compound fertilizer in suppression of Sclerotinia sclerotiorum[J]. Biological Control, 2011, 59(2):221-227.