Function Analysis of LysR and GntR Family Genes in Lysobacter enzymogenes OH11

doi:10.16409/j.cnki.2095-039x.2021.01.003

Abstract

Abstract: Lysobacter enzymogenes OH11 can approach and adhere to the hypha of pathogenic fungi and oomycetes through twitching motility. Then OH11 secretes enzymes and secondary metabolites including HSAF (heat-stable antifungal factor), which is a new type of anti-fungal secondary metabolite and a potential resource for new bio-control fungicide, to inhibit pathogen growth. Transcription factor (TF) plays a key role in regulation of gene expression. LysR and GntR TF family regulate motility and pathogenicity in pathogenic bacteria. In this paper, we knocked out encoding genes of 3 LysR TFs (Le1404, Le2114 and Le3293) and 2 GntR TFs (Le1155 and Le1310) to investigate whether they regulate twitching motility and HSAF biosynthesis. ΔLe2114 and ΔLe1155 showed defect in twitching motility; ΔLe1155 produced lower amount of HSAF compared with wild-type. These results indicated Le2114 specifically regulated twitching motility and Le1155 regulated both twitching motility and HSAF biosynthesis.

Key words: Lysobacter enzymogenes, twitching motility, HSAF, LysR, GntR

CLC Number:

S476

LIN Long, GE Chengcheng, JIANG Jiandong, QIAN Guoliang. Function Analysis of LysR and GntR Family Genes in Lysobacter enzymogenes OH11[J]. Chinese Journal of Biological Control, 2021, 37(1): 130-138.

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