Electroantennogram and Behavioral Responses of Colposcelis signata Adults to Non-host Plant Volatiles

doi:10.16409/j.cnki.2095-039x.2021.03.001

Abstract

Abstract: The antennal and behavioral responses of Colposcelis signata adults to sixteen volatile compounds from non-host plants were measured using electroantennogram (EAG) and Y tube olfactometer to explore for behavioral manipulation agents for C. signata adults. The results showed that C. signata adults had strong EAG responses and obvious repellent responses to 6 terpenes volatile compounds at concentrations of 10^-1mol/L, the responses being stronger in males than females. The EAG responses to (1R)-(+)-α-pinene and (-)-β-pinene were significantly stronger than those to the other four terpenes, and the two compounds elicited male repellent rates of 86.7% and 81.3%, respectively. Colposcelis signata showed significantly different EAG responses to the five alcohols and aldehydes volatiles, which were all attractive responses except for the response to linalool. Compared to the males, the females exhibited significantly stronger attractive responses to the three green leaf volatiles, trans-2-hexen-1-ol, 2-ethyl-1-hexanol, and trans-2-hexenal. The attractive rate of trans-2-hexen-1-ol to the females was the highest, 68.9%. These green leaf volatiles may be related to the location of oviposition sites in this insect. Adults showed relatively low EAG responses and attractive responses to 5 other volatiles, but stronger in males than in females. Both females and males showed higher EAG responses and attractive responses to methyl salicylate than to the other 4 volatiles. The concentration response results indicated that, when the concentration increased from 10^-2mol/L to 10^-1mol/L, the EAG responses of adults to (1R)-(+)-α-pinene and (-)-β-pinene decreased slightly, and the repellent responses of adults to (-)-β-pinene and β-myrcene decreased and showed a concentration threshold. However, (1R)-(+)-α-pinene, (-)-β-pinene, (R)-(+)-limonene and β-myrcene even exhibited obvious repellent effects at a concentration of 10^-5mol/L. Therefore, these volatiles can be exploited as repellent components.

Key words: Colposcelis signata, non-host plant volatiles, electroantennogram (EAG), behavioral response

CLC Number:

S476

FAN Ruidong, ZHANG Yikai, BI Jiarui, XU Wei, ZANG Liansheng. Electroantennogram and Behavioral Responses of Colposcelis signata Adults to Non-host Plant Volatiles[J]. Chinese Journal of Biological Control, 2021, 37(4): 740-748.

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