Abstract: The pr1 gene, a potential virulence factor by virtue of its activity against insect cuticles, was cloned from isolate M2189 of Metarhizium anisopliae,
highly virulent to locusts. Expected DNA fragments were amplified by DNA-PCR and mRNA-RT-PCR respectively with a pair of specific primers. The fragments were introduced into isolate JM109 of Escherichia coli by pGEMT easy vector. Integrated DNA from recombinant plasmid was identified by electrophoresis after restriction enzyme digestion. Nucleotide of the expected DNA fragment was sequenced. Result showed that the fragment was 1369 bp in full length, in which there were 3 introns which were 76, 59 and 67 bp, respectively. Proteinencoding part was 1167 bp in length containing a full protein reading frame with an initiation codon and a termination codon at the two ends. The nucleotide number of the proteinencoding sequence was the same as that of pr1 from isolate Me1 of M. anisopliae published by St. Leger, and identity of their nucleotide get to 92.2%. The two sequences predicted a protein of 389 amino acids respectively， and there are 48 different amino acids between them amounting to 12.3% of the total.