Abstract: 1975 soil samples were collected from different areas in Hebei Province and the entomopathogenic nematodes (EPN) were extracted with Galleria mellonella trap method. A total of 66 populations were isolated from soil samples, among which 2 populations are Steinernema glaseri, 49 populations are Heterorhabditis bacteriophora. One population (G6) isolated from Gu'an county is a new steinernematide species that can be distinguished from all known species by morphology, morphometrics and ITSrDNA sequence analysis, and the others were confirmed to be another new steinernematide species. It was determined that occurrence of EPN was closely related to plant species and soil types. The prevalence of EPN in uncultivated soil, orchard, vegetable fields and crop fields were 6.59%, 5.13%, 3.15% and 2.90%, respectively; and in sandy, sandy loam, loam and clay soils, 8.57%, 3.17%, 3.36% and 0, respectively.