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Resistance of Transgenic Bt Poplar to Clostera anachorta

KANG Wei1;ZHENG Jin2;LI Bing1;HONG Hua-zhu1
  

  1. 1.Key Laboratory of Pesticide and Chemical Biology;Ministry of Education,Central China Normal University Wuhan 430079;2.Huangshi Institute of Technology,Huangshi 435003,China
  • Received:2008-07-01 Revised:1900-01-01 Online:2009-05-08 Published:2009-05-08
  • Contact: HONG Hua-zhu

Abstract: Bio-assays and tissue section examinations were conducted for determining the resistance of transgenic poplar(Populus deltoides,1-63×1-69)expressing Bt CrylA toxin to Clostera anachorta.The results showed that larvae fed with the transgenic poplar leaves showed increasing mortalities and weight inhibition rates with the extension of exposure period,being 10%and 6.85%at 2 d,48.33%and 27.93%at 4 d,and 81.67%,63.03%at 10 d,respectively. Pupation rate of larvae fed with non-Bt and Bt transgenic poplar for 12 d was 100%and 18.18%,respectively.The midgut epithelium cell of the larvae exposed to transgenic popular was loose and partially vacuolar,and the cell boundary was vague.It is suggested that the transgenic Bt poplar shows insecticidal and growth-inhibitory effects on C. anachorta.
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