Abstract: Hpfr1, a homological sequence of hrf1 , was cloned from a japonica cultivar R109 by PCR analysis. The homologies of hpfr1 compared with hrf1 are 92% and inferred homologies of amino acid from hpfr1 compared with that from hrf1 are 74.8%. Hpfr1 gene was ligated into expression vector pET30a(+) which has T7 promoter to construct recombinant plasmid pHOSJ4. The function of this protein is similar to harpin_Xoo and causes HR in non-host plant. The seguences of hpfr1 and hrf1 are the same from the initiation code ATG to 225 bp site. The sequences from ATG to 225 bp site were cloned and constructed expression vector pHOSJ4-225. The crude recombinant proteins also cause HR in Xanthi tobacco. Sprays of the proteins onto Xanthi tobacco induce the resistance of tobacco to TMV remarkably. The contrast analyses of LSD indicate that the protein extraction of BL21 (DE3)/pHOSJ4 and BL21(DE3)/pHOSJ4-225 can dramatically enhance tobacco resistance to TMV.