藿香精油对朱砂叶螨的触杀毒力及几种保护酶活性的影响

doi:10.16409/j.cnki.2095-039x.2016.06.002

摘要/Abstract

摘要： 本研究旨在探明藿香精油对朱砂叶螨的触杀毒力及几种保护酶活性的影响。采用喷雾法测定了藿香精油对朱砂叶螨5日龄雌成螨和卵的触杀毒力及雌成螨的产卵抑制作用，分析比较了LC₃₀、LC₅₀和LC₉₀藿香精油喷雾处理雌成螨4、8、12、16、20和24 h后，其体内蛋白酶和3种保护酶活性的变化。结果表明，经藿香精油处理后，雌成螨的死亡率和卵的孵化抑制率与供试浓度呈正相关，LC₅₀和IC50分别为0.60%、0.62%。随着供试浓度的增加，藿香精油的触杀作用增强，对雌成螨的产卵抑制率和产卵抑制指数呈上升趋势，且对雌成螨的触杀作用高于卵。用浓度LC₃₀、LC₅₀和LC₉₀藿香精油处理雌成螨4 h后，蛋白酶和POD活性被激活，SOD活性被抑制，蛋白酶的激活率分别为10.96%、47.70%和62.92%，POD活性分别为对照的2.3、1.5和0.3倍，SOD的抑制率分别为44.52%、23.91%和49.08%。LC₃₀和LC₅₀处理4 h后CAT活性被激活，激活率分别为20.26%和52.22%；LC₉₀处理后CAT活性被抑制，抑制率为62.21%。藿香精油对朱砂叶螨有一定的触杀毒力，能有效抑制雌成螨产卵，可通过扰乱蛋白酶和保护酶的动态平衡来影响其正常生理代谢，从而起到毒害作用。因此，藿香精油在农业防治朱砂叶螨方面有一定的开发潜力。

关键词: 藿香精油, 朱砂叶螨, 触杀毒力, 蛋白酶, 保护酶

Abstract: This study aims to determine the contact toxicity of Agastache rugosus essential oil against Tetranychus cinnabarinus and its effects on several protective enzymes activities. The contact toxicity of A. rugose essential oil against 5-day-old old female adults and eggs, and its oviposition inhibition activities were measured by spray application. The activities of protease and three protective enzymes (SOD, POD and CAT) were also measured in the female adults treated with the essential oil at three different concentrations (LC₃₀, LC₅₀ and LC₉₀) at 4, 8, 12, 16, 20 and 24 h post treatment. The mortality of female adults and inhibition of egg incubation were positively correlated with the concentrations applied, for which LC₅₀ values were 0.60% and 0.62%, respectively. Moreover, with the increasing of concentration, the contact toxicity, the oviposition inhibition rate and index all increased. Female adults were more sensitive to the treatment than eggs. At 4 h after treatment with different doses, protease and POD were activated, while SOD activity was inhibited. Protease was activated by 10.96%, 47.70% and 62.92% at the LC₃₀, LC₅₀ and LC₉₀, respectively; POD activities were 2.3, 1.5 and 0.3 times higher than those in the control group, respectively; SOD inhibition rates were 44.52%, 23.91% and 49.08%, respectively. CAT activities were activated at LC₃₀ and LC₅₀, the activation rates were 20.26% and 52.22%, respectively; at LC₉₀, CAT activity was inhibited by 62.21%. It is obvious that A. rugosus essential oil has a potential to kill the female adults and inhibit oviposition, which may be the results of abnormal physiological metabolism of protease and protective enzymes. Therefore, A. rugosus essential oil might be developed into a control agent against T. cinnabarinus.

Key words: Agastache rugosus essential oil, Tetranychus cinnabarinus, contact toxicity, protease, protective enzymes

中图分类号:

Q965.9

马新耀, 程作慧, 刘耀华, 李锐, 李生才. 藿香精油对朱砂叶螨的触杀毒力及几种保护酶活性的影响[J]. 中国生物防治学报, 2016, 32(6): 689-697.

MA Xinyao, CHENG Zuohui, LIU Yaohua, LI Rui, LI Shengcai. Contact Toxicities of Agastache rugosus Essential Oil against Tetranychus cinnabarinus (Acari: Tetranychidae) and Effects on Several Protective Enzymes Activities[J]. journal1, 2016, 32(6): 689-697.

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