野生和栽培茭白二化螟感病率与微生物多样性分析

doi:10.16409/j.cnki.2095-039x.2020.02.004

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (2): 189-195.DOI: 10.16409/j.cnki.2095-039x.2020.02.004

野生和栽培茭白二化螟感病率与微生物多样性分析

刘樾¹, 郭昊¹, 黄新芳², 张小丽¹, 李多¹, 王永模¹

1. 华中农业大学植物科学技术学院, 武汉 430070;
2. 武汉市农业科学院蔬菜研究所, 武汉 430345

收稿日期:2019-09-18 出版日期:2020-04-08 发布日期:2020-04-16
通讯作者: 王永模,副教授,E-mail:ymwang@mail.hzau.edu.cn
作者简介:刘樾,硕士研究生,E-mail:1994346353@qq.com
基金资助:
国家重点研发计划（2017YFD0201103）；武汉市农业科学院开放性课题（KFKT201903）

Morbidity of Chilo suppressalis in Wild and Cultivated Water Oat (Zizania latifolia) and Microorganism Diversity in Dead Insects

LIU Yue¹, GUO Hao¹, HUANG Xinfang², ZHANG Xiaoli¹, LI Duo¹, WANG Yongmo¹

1. College of plant science & technology, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Vegetables, Wuhan Institute of Agricultural Sciences, Wuhan 430345, China

Received:2019-09-18 Online:2020-04-08 Published:2020-04-16

摘要/Abstract

摘要： 为了明确茭白二化螟的感病死亡率以及携带的优势微生物种类，本文在野生茭白和栽培茭白中共采集二化螟越冬幼虫6000余头，发现二化螟的感病死亡率分别为19.98%和17.00%，二者之间没有显著差异，表明栽培措施没有影响昆虫病原菌的流行。采用18S rRNA和16S rRNA测序方法对死虫标本中的真菌和细菌组成和丰度进行了研究，结果表明95%以上个体的优势真菌为白僵菌，相对丰度在90%以上，没有检出绿僵菌；优势细菌科为肠杆菌科、肠球菌科、根瘤菌科和丛毛单胞菌科，丰度分别为19.37%、17.37%、7.04%和5.04%，含昆虫病原细菌的芽胞杆菌科和梭菌科细菌的丰度只有1.44%和0.41%，表明细菌不是致死的主要原因。本研究证明白僵菌是越冬期茭白二化螟的优势病原菌。因此，在茭白田中开展以菌治虫首选使用白僵菌。

关键词: 茭白, 二化螟, 生物防治, 昆虫病原菌, 白僵菌

Abstract: Water oat Zizania latifolia is an aquatic vegetable in China, with high nutritional value and good taste. In recent years, the vegetable has been cultivated increasingly. However, water oat is susceptible to insect pests among which Chilo suppressalis is one of the most serious. Insect pathogens are effective biological control agents of pest insects. In order to find effective insect pathogens that prevail in water oat field, 6039 overwintering C. suppressalis larvae were collected from wild and cultivated water oat, and were observed for the mortality due to insect pathogens, which revealed no difference in pathogenic mortality of 19.98%in wild water oat and of 17.00% in cultivated water oat, indicating that farming practices do not affect the prevailing of insect pathogens. Microorganism community in the dead specimens (>1000) were identified by high-throughput sequencing of 18S rRNA and 16S rRNA gene. Results showed that 96.67% of dead specimens from wild water oat were predominated by Beauveria fungi, and 100% of dead specimens from cultivated water oat were predominated by this genus. Except for one sample, the abundance of Beauveria in each specimen was more than 90%. Metarhizium fungi were not detected in any of the specimens. Enterobacteriaceae, Rhizobiaceae, Enterococcaceae and Comamonadaceae were the top four bacteria family with abundances of 19.37%, 17.37%, 7.04% and 5.04%, respectively. Insect pathogenic bacteria were seldom reported in those four dominant families. Bacillaceae and Clostridiaceae that most likely contain insect pathogenic bacteria were only 1.44% and 0.41% in abundance. This study shows that Beauveria is the dominant pathogen of C. suppressalis during the overwintering stage, and is of potential as biocontrol agent against C. suppressalis in water oat field.

Key words: Zizania latifolia, Chilo suppressalis, biological control, insect pathogen, Beauveria

中图分类号:

S476.1

刘樾, 郭昊, 黄新芳, 张小丽, 李多, 王永模. 野生和栽培茭白二化螟感病率与微生物多样性分析[J]. 中国生物防治学报, 2020, 36(2): 189-195.

LIU Yue, GUO Hao, HUANG Xinfang, ZHANG Xiaoli, LI Duo, WANG Yongmo. Morbidity of Chilo suppressalis in Wild and Cultivated Water Oat (Zizania latifolia) and Microorganism Diversity in Dead Insects[J]. Chinese Journal Of Biological Control, 2020, 36(2): 189-195.

参考文献

[1] 刘义满, 柯卫东, 钟兰, 等. 茭白北移栽培的有关技术问题[J]. 中国蔬菜, 2010, 1(23):48-50.
[2] Huang C, Hu B, Li J, et al. Water-oats harbors two strains of the striped stem borer Chilo suppressalis (Lepidoptera:Crambidae) with temporal divergence in mating behavior[J]. Applied Entomology and Zoology, 2016, 51(3):457-463.
[3] 胡美华, 俞朝, 沈学根等. 茭白田应用性诱剂防治二化螟试验初报[J]. 长江蔬菜, 2009(16):62-64.
[4] Lacey L A, Frutos R, Kaya H K, et al. Insect pathogens as biological control agents:do they have a future?[J]. Biological Control, 2001, 21(3):230-248.
[5] Leger R J S, Wang C, Fang W. New perspectives on insect pathogens[J]. Fungal Biology Reviews, 2011, 25(2):84-88.
[6] Jaber L R, Ownley B H. Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens?[J]. Biological Control, 2017, 107:50-59.
[7] 蒲蛰龙, 李增智. 昆虫真菌学[M]. 合肥:安徽科学技术出版社, 1996.
[8] 何恒果, 李正跃, 陈斌, 等. 虫生真菌对害虫防治的研究与应用[J]. 云南农业大学学报, 2004, 19(2):167-173.
[9] 来有鹏, 张登峰. 白僵菌和绿僵菌在农业害虫防治中的应用研究进展[J]. 青海农林科技, 2011(1):40-42.
[10] 张小霞, 尹新明, 梁振普. 害虫生物防治技术基础与应用[M]. 北京:科学出版社, 2010.
[11] Macielvergara G, Jensen A, Eilenberg J. Cannibalism as a possible entry route for opportunistic pathogenic bacteria to insect hosts, exemplified by Pseudomonas aeruginosa, a pathogen of the giant mealworm Zophobas morio[J]. Insects, 2018, 9(3):88.
[12] AndreʼLuiz de Almeida Melo, Soccol V T, Soccol C R. Bacillus thuringiensis:mechanism of action, resistance, and new applications:a review[J]. Critical Reviews in Biotechnology, 2016, 36(2):317-326.
[13] 束长龙, 曹蓓蓓, 袁善奎, 等. 微生物农药管理现状与展望[J]. 中国生物防治学报, 2017, 33(3):4-10.
[14] 符泽, 邱光. 微生物农药的研发推广及未来发展趋势[J]. 农药市场信息, 2018(6):6-10.
[15] Lacey L A, ed. Manual of Techniques in Insect Pathology[M]. San Diego:Academic Press, 1997.
[16] Klingen I, Eilenberg J, Meadow R. Effects of farming system, field margins and bait insect on the occurrence of insect pathogenic fungi in soils[J]. Agriculture, Ecosystems & Environment, 2002, 91(1-3):191-198.
[17] Vogelweith F, Thiéry D, Quaglietti B, et al. Host plant variation plastically impacts different traits of the immune system of a phytophagous insect[J]. Functional Ecology, 2011, 25(6):1241-1247.
[18] Wang Y, Huang C, Hu B, et al. Gene flow across host-associated populations of the rice stem borer Chilo suppressalis Walker (Lepidoptera:Crambidae)-implications for Bt resistance management in rice[J]. Pest Management Science, 2019, 76(2):695-703.
[19] 艾铄, 张丽杰, 肖芃颖, 等. 高通量测序技术在环境微生物领域的应用与进展[J]. 重庆理工大学学报(自然科学), 2018, 32(9):117-127.
[20] 李亦菲, 郑亚强, 徐天梅, 等. 西双版纳自然保护区白僵菌属虫生真菌多样性[J]. 中国生物防治学报, 2018, 34(4):64-72.
[21] 陈名君, 章西, 侯囡嵩, 等. 安徽大别山区绿僵菌属物种多样性[J]. 中国生物防治学报, 2018, 34(2):274-279.
[22] Kwak K W, Nam S H, Choi J Y, et al. Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis[J]. International Journal of Industrial Entomology, 2015, 30(2):64-74.
[23] Imoulan A, Hussain M, Kirk P M, et al. Entomopathogenic fungus Beauveria:Host specificity, ecology and significance of morpho-molecular characterization in accurate taxonomic classification[J]. Journal of Asia-Pacific Entomology, 2017, 20(4):1204-1212.
[24] Lo Verde G, Torta L, Mondello V, et al. Pathogenicity bioassays of isolates of Beauveria bassiana on Rhynchophorus ferrugineus[J]. Pest Management Science, 2015, 71(2):323-328.
[25] 农向群, 张泽华. 昆虫病原真菌的生态适应性及其生物防治应用策略[J]. 中国生物防治学报, 2013, 29(1):133-141.

野生和栽培茭白二化螟感病率与微生物多样性分析

Morbidity of Chilo suppressalis in Wild and Cultivated Water Oat (Zizania latifolia) and Microorganism Diversity in Dead Insects

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