内生解淀粉芽胞杆菌BEB33脂肽类化合物分析及对香蕉枯萎病菌拮抗作用研究

doi:10.16409/j.cnki.2095-039x.2019.05.022

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (5): 785-792.DOI: 10.16409/j.cnki.2095-039x.2019.05.022

内生解淀粉芽胞杆菌BEB33脂肽类化合物分析及对香蕉枯萎病菌拮抗作用研究

王宝^1,2, 杨扬², 蔡吉苗², 陈奕鹏², 黄贵修²

1. 华中农业大学植物科学技术学院, 武汉 430070;
2. 中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室/海南省热带作物病虫害生物防治工程技术研究中心, 海口 571101

收稿日期:2019-02-18 出版日期:2019-10-08 发布日期:2019-10-26
通讯作者: 黄贵修,博士,研究员,E-mail:hgxiu@vip.163.com。
作者简介:王宝,硕士研究生,E-mail:924740138@qq.com
基金资助:
国家重点研发计划（2017YFD0202104）；中央级公益性科研院所基本业务费专项（1630042018016）

Identification of Lipopeptides of Endophytic Bacillus amyloliquefaciens BEB33 and Antifunal Activity against Fusarium oxysporum f. sp. cubense

WANG Bao^1,2, YANG Yang², CAI Jimiao², CHEN Yipeng², HUANG Guixiu²

1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, P. R. China/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests/Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Received:2019-02-18 Online:2019-10-08 Published:2019-10-26

摘要/Abstract

摘要： 为解析内生解淀粉芽胞杆菌Bacillus amyloliquefaciens BEB33的生防机理，检测了该菌株脂肽类化合物合成相关基因，利用基质协助激光解吸附离子化-飞行时间质谱分析了该菌株脂肽类化合物的种类，评价了脂肽类粗提物对香蕉枯萎病菌（FOC4）形态、镰刀菌酸合成的影响，以及粗提物对香蕉枯萎病的生防潜力分析。结果显示，BEB33基因组中编码有surfactin、fengycin、iturin和bacillomycin D 4种脂肽类物质合成酶基因。使用M9培养基发酵能产生surfactin、fengycin和bacillomycin D 3种脂肽类物质；脂肽类粗提物可导致香蕉枯萎病菌菌丝细胞膨大，细胞膜穿孔，镰刀菌酸产量降低70%；并发现脂肽类粗提物对香蕉枯萎病具有明显的防治效果。在不同处理中，枯萎病发病率分别降低47.67%、23.22%和36.56%，病情指数分别降低38.09、28.56和35.87。本文所获研究结果为进一步利用该菌株进行香蕉枯萎病生防制剂的研发提供理论基础。

关键词: 内生细菌, 解淀粉芽胞杆菌, 脂肽类化合物, 香蕉枯萎病菌

Abstract: To investigate biocontrol mechanism of endophytic Bacillus amyloliquefaciens strain BEB33, the genes related to the synthesis of lipopeptides were detected and the lipopeptides were analyzed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The effects of crude lipopeptide extracts on the morphology of Fusarium oxysporum f. sp. cubense race4 (FOC4) and fusaric acid synthesis were investigated, and the biocontrol potential of crude extracts against banana wilt was also evaluated. The results showed that there 4 lipopeptide synthases (surfactin, fengycin, iturin and bacillomycin D) were detected in BEB33 genome. However, only three different lipopeptides (surfactin, fengycin and bacillomycin D) were detected in BEB33 lipopeptide crude extraction from M9 culture of BEB33. After treatment with BEB33 lipopeptide crude extraction, FOC4 hyphae became enlargement and could be dyed with trypan blue. The crude lipopeptide extract could reduce the yield of fusaric acid by 70%. The crude lipopeptide extract had obvious control effect on banana wilt. The disease incidence was reduced by 47.67%, 23.22% and 36.56%, respectively, and the disease index was decreased by 38.09, 28.56 and 35.87, respectively. These results provide a theoretical basis for further research and development of biocontrol agents for banana wilt disease.

Key words: endophytic bacteria, Bacillus amyloliquefaciens, lipopeptide, Fusarium wilt of banana

中图分类号:

S476

王宝, 杨扬, 蔡吉苗, 陈奕鹏, 黄贵修. 内生解淀粉芽胞杆菌BEB33脂肽类化合物分析及对香蕉枯萎病菌拮抗作用研究[J]. 中国生物防治学报, 2019, 35(5): 785-792.

WANG Bao, YANG Yang, CAI Jimiao, CHEN Yipeng, HUANG Guixiu. Identification of Lipopeptides of Endophytic Bacillus amyloliquefaciens BEB33 and Antifunal Activity against Fusarium oxysporum f. sp. cubense[J]. Chinese Journal Of Biological Control, 2019, 35(5): 785-792.

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内生解淀粉芽胞杆菌BEB33脂肽类化合物分析及对香蕉枯萎病菌拮抗作用研究

Identification of Lipopeptides of Endophytic Bacillus amyloliquefaciens BEB33 and Antifunal Activity against Fusarium oxysporum f. sp. cubense

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