木寡糖提高拟南芥抗病性

doi:10.16409/j.cnki.2095-039x.2021.01.030

摘要/Abstract

摘要： 木寡糖是一种植物源寡糖，对动物体的生理功能多且显著，而其是否作为寡糖素诱发植物防卫尚不明确。本文分析了不同浓度木寡糖诱导拟南芥植株对不同营养型病原的抗性。结果表明，木寡糖能诱发拟南芥对半活体病原细菌丁香假单胞菌番茄致病变种、活体病原烟草花叶病毒和死体病原真菌核盘菌的抗性，且具有剂量依赖效应，随着木寡糖浓度的提高到100 μg/mL，对Pst DC3000和TMV诱导抗病效果逐渐增强；当木寡糖浓度提高到50 μg/mL，对核盘菌的诱导抗病效果不再升高；说明木寡糖是一种新的可诱发拟南芥广谱抗病性的寡糖素。本研究为进一步解析木寡糖诱发植物免疫的信号传导和开发新的病害控制策略提供了依据。

关键词: 木寡糖, 寡糖素, 抗病性, 病原

Abstract: Plant-origined xylo-oligosaccharides (XOS) have many excellent physiological and biological properties in animals. However, whether XOS acts as an oligosaccharins to induce plant defense remains unclear. In this study, the induced disease resistance to different pathogens upon XOS with different concentrations was investigated. Here, we found that XOS can induce plant resistance against hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000, biotrophic pathogen Tobacco mosaic virus and necrotrophic pathogen Sclerotinia sclerotiorum in a dose-dependent manner. The induced resistance effect to Pst DC3000 and TMV increased as XOS concentration increased to 100 μg/mL. While XOS concentration increased to 50 μg/mL, the induced resistance effect to S. sclerotiorum was no longer rising. The results indicated that another new biotic sugar, XOS, can broadly elicit disease resistance in Arabidopsis, which provide a basis for elucidating the signal transduction of disease resistance induced by XOS and designing efficient strategies for plant disease control.

Key words: xylo-oligosaccharides, oligosaccharins, disease resistance, pathogen

中图分类号:

S476

缪金露, 张华建. 木寡糖提高拟南芥抗病性[J]. 中国生物防治学报, 2021, 37(1): 150-155.

MIU Jinlu, ZHANG Huajian. Xylo-oligosaccharides Elicits Disease Resistance in Arabidopsis[J]. Chinese Journal of Biological Control, 2021, 37(1): 150-155.

参考文献

[1] Zhang W, Zhao F, Jiang L, et al. Different pathogen defense strategies in Arabidopsis:more than pathogen recognition[J]. Cells, 2018, 7(12):252.
[2] Rocafort M, Fudal I, Mesarich C H. Apoplastic effector proteins of plant-associated fungi and oomycetes[J]. Current Opinion in Plant Biology, 2020, 56:9-19.
[3] Fonseca J P, Mysore K S. Genes involved in nonhost disease resistance as a key to engineer durable resistance in crops[J]. Plant Science, 2019, 279:108-116.
[4] Ingle R A, Carstens M, Denby K J. PAMP recognition and the plant-pathogen arms race[J]. Bioessays, 2006, 28(9):880-889.
[5] Garcia-Brugger A, Lamotte O, Vandelle E, et al. Early signaling events induced by elicitors of plant defenses[J]. Molecular Plant-Microbe Interactions, 2006, 19(7):711-724.
[6] Darvill A G, Albersheim P, McNeil M, et al. Structure and function of plant cell wall polysaccharides[J]. Journal of Cell Science, 1985, 2(S):203-217.
[7] Gravino M, Savatin D V, Macone A, et al. Ethylene production in Botrytis cinerea- and oligogalacturonide-induced immunity requires calcium-dependent protein kinases[J]. Plant Journal, 2015, 84(6):1073-1086.
[8] Brutus A, Sicilia F, Macone A, et al. A domain swap approach reveals a role of the plant wall-associated kinase 1(WAK1) as a receptor of oligogalacturonides[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(20):9452-9457.
[9] Sharp J K, Valent B, Albersheim P. Purification and partial characterization of a beta-glucan fragment that elicits phytoalexin accumulation in soybean[J]. Journal of Biological Chemistry, 1984, 259(18):11312-11320.
[10] Sharp J K, McNeil M, Albersheim P. The primary structures of one elicitor-active and seven elicitor-inactive hexa(beta-D-glucopyranosyl)-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f. sp. glycinea[J]. Journal of Biological Chemistry, 1984, 259(18):11321-11336.
[11] Yamaguchi T, Yamada A, Hong N, et al. Differences in the recognition of glucan elicitor signals between rice and soybean:beta-glucan fragments from the rice blast disease fungus Pyricularia oryzae that elicit phytoalexin biosynthesis in suspension-cultured rice cells[J]. Plant Cell, 2000, 12(5):817-826.
[12] Brule D, Villano C, Davies L J, et al. The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide- triggered immunity[J]. Plant Biotechnology Journal, 2018, 17(4):812-825.
[13] Azevedo Carvalho A F, de Oliva Neto P, Da Silva D F, et al. Xylo-oligosaccharides from lignocellulosic materials:Chemical structure, health benefits and production by chemical and enzymatic hydrolysis[J]. Food Research International, 2013, 51:75-85.
[14] Aachary A A, Prapulla S G. Xylooligosaccharides (XOS) as an emerging prebiotic:microbial synthesis, utilization, structural characterization, bioactive properties, and applications[J]. Comprehensive Reviews in Food Science and Food Safety, 2011, 10:2-16.
[15] Broekaert W F, Courtin C M, Verbeke K, et al. Prebiotic and other health-related effects of cereal-derived arabinoxylans, arabinoxylan-oligosaccharides, and xylooligosaccharides[J]. Critical Reviews in Food Science and Nutrition, 2011, 51(2):178-194.
[16] Yuan L, Li W, Huo Q, et al. Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens[J]. Peerj, 2018, 6:e4435.
[17] Gibson G R. From probiotics to prebiotics and a healthy digestive system[J]. Journal of Food Science, 2004, 69(5):141-143.
[18] Nabarlatz D, Montane D, Kardosova A, et al. Almond shell xylo-oligosaccharides exhibiting immunostimulatory activity[J]. Carbohydrate Research, 2007, 342(8):1122-1128.
[19] Chen W, Guo C, Hussain S, et al. Role of xylo-oligosaccharides in protection against salinity-induced adversities in Chinese cabbage[J]. Environmental Science and Pollution Research, 2016, 23(2):1254-1264.
[20] Steffen J G, Kang I H, Portereiko M F, et al. AGL61 interacts with AGL80 and is required for central cell development in Arabidopsis[J]. Plant Physiology, 2008, 148(1):259-268.
[21] Htet A N, Noguchi M, Ninomiya K, et al. Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid[J]. Journal of Bioscience and Bioengineering, 2018, 125(6):717-722.
[22] Chen L, Zhang S J, Zhang S S, et al. A fragment of the Xanthomonas oryzae pv. oryzicola harpin HpaG (Xooc) reduces disease and increases yield of rice in extensive grower plantings[J]. Phytopathology, 2008, 98(7):792-802.
[23] Le M H, Cao Y, Zhang X C, et al. LIK1, a cerk1-interacting kinase, regulates plant immune responses in Arabidopsis[J]. Plos One, 2014, 9(7):e102245.
[24] Jia X, Meng Q, Zeng H, et al. Chitosan oligosaccharide induces resistance to tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway[J]. Scientific Reports, 2016, 6:26144.
[25] Jia X, Zeng H, Wang W, et al. Chitosan oligosaccharide induces resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana by activating both salicylic acid- and jasmonic acid-mediated pathways[J]. Molecular Plant-Microbe Interactions, 2018, 31(12):1271-1279.
[26] Liu J, Zhang X, Kennedy J F, et al. Chitosan induces resistance to tuber rot in stored potato caused by Alternaria tenuissima[J]. International Journal of Biological Macromolecules, 2019, 140:851-857.