洋葱伯克霍尔德氏菌JX-1防治番茄青枯病机理的初步分析

doi:10.16409/j.cnki.2095-039x.2021.02.003

摘要/Abstract

摘要： 本研究分离得到一株对茄科雷尔氏菌Ralstonia solanacearum具有明显拮抗作用的根围细菌JX-1，其温室防治番茄青枯病的防效达80.89%。形态学、生理生化、16S rDNA序列和gyrB序列分析表明，该菌株属于洋葱伯克霍尔德氏菌Burkholderia cepacia。生防相关性状分析表明，菌株JX-1可产生嗜铁素、蛋白酶等抗菌物质。抗生素合成相关基因分析发现，菌株JX-1还可能产生硝吡咯菌素和藤黄绿脓菌素。转座子随机突变获得2株明显影响菌株JX-1拮抗能力的突变菌株，其中突变体M1710对茄科雷尔氏菌拮抗作用完全消失，序列分析表明Tn5破坏了与非核糖体寡肽产生相关基因tlpks/nrps；而突变体M645则对茄科雷尔氏菌的拮抗作用显著增强，序列分析表明Tn5破坏了gntR基因。上述结果表明，菌株JX-1可产生多种次生代谢产物，并且tlpks/nrps基因和gntR基因在防治番茄青枯病过程中起到重要调整作用。

关键词: Burkholderia cepacia, 番茄青枯病, 拮抗作用, GntR转录调控因子

Abstract: Strain JX-1, which was isolated form the rhizosphere soil, exhibited high levels of biocontrol efficacy against tomato bacterial wilt caused by Ralstonia solanacearum under greenhouse conditions. Strain JX-1 was identified as Burkholderia cepacia based on morphological, physio-biochemistry features, and sequence analysis of 16S rDNA and gyrB. Strain JX-1 produces several antimicrobial compounds, such as siderophores and extracellular proteinase. PCR analysis demonstrated that the specific genes within the biosynthetic loci for pyrrolnitrin and pyoluterin are conserved in strain JX-1. Random mutagenesis was performed to identify novel regulator of antimicrobial traits in strain JX-1, and the mutants with significantly increased or decreased the antimicrobial activity were identified. Our data showed that the antimicrobial activity was negatively regulated by gntR gene, and positively influenced by the tlpks/nrps gene in strain JX-1. Our findings indicated that JX-1 could produce multiple secondary metabolites and the tlpks/nrps and gntR genes played a vital role on its biocontrol capacity.

Key words: Burkholderia cepacia, tomato bacterial wilt, antagonism, GntR transcriptional regulator

中图分类号:

S476.3

许萌杏, 李凤芳, 袁高庆, 黎起秦, 吴小刚. 洋葱伯克霍尔德氏菌JX-1防治番茄青枯病机理的初步分析[J]. 中国生物防治学报, 2021, 37(2): 304-314.

XU Mengxing, LI Fengfang, YUAN Gaoqing, LI Qiqin, WU Xiaogang. Identification and Characterization of Burkholderia cepacia JX-1 against the Tomato Bacterial Wilt[J]. Chinese Journal of Biological Control, 2021, 37(2): 304-314.

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