广双瘤蚜茧蜂对棉蚜脂质合成通路的影响

doi:10.16409/j.cnki.2095-039x.2021.03.007

摘要/Abstract

摘要： 棉蚜是棉花的重要害虫，广双瘤蚜茧蜂是棉蚜的重要寄生性天敌，可有效控制棉蚜的数量。寄生蜂调节寄主的脂质合成代谢已多有报道，但在广双瘤蚜茧蜂与棉蚜的相互作用中脂质合成相关基因的变化尚不清楚。本研究针对脂肪酸合成通路、甘油酯合成通路、糖酵解以及氨基酸代谢通路共包含36个与棉蚜脂质合成相关的基因，利用实时荧光定量PCR技术研究了其在被寄生棉蚜体内的转录变化。结果表明，被广双瘤蚜茧蜂寄生后的棉蚜，2个糖酵解通路基因、5个脂肪酸合成通路基因、6个甘油酯合成通路基因和5个氨基酸代谢通路的关键基因全部显著上调，其中甘油酯合成通路中的甘油激酶表达量显著性下降，其他基因表达量均无显著性变化。本研究明确了广双瘤蚜茧蜂对棉蚜脂质合成的影响，该结果对深入了解寄生蜂与寄主互作关系，指导人工繁育寄生蜂和害虫防控具有重要的理论和实践价值。

关键词: 棉蚜, 广双瘤蚜茧蜂, 脂质合成, 荧光定量PCR, 害虫防控

Abstract: Cotton aphid, Aphis gossypii Glover is an important pest of cotton, and the wasp, Binodoxys communis Gahan is an important naturally parasitic enemy of A. gossypii, which can effectively control the quantity of A. gossypii. It has been reported that parasitic wasps regulate the lipid anabolism of their hosts, but the changes of lipid-synthesis-related genes in the interaction between B. communis and A. gossypii are still unclear. In this study, we focused on the fatty acid synthesis pathway, the glyceride synthesis pathway, the glycolysis pathway and the amino acid metabolism pathway contained 36 genes related to the lipid synthesis of cotton aphid, and the real-time fluorescence quantitative PCR technology was used to study the transcription changes of these genes in the body of the parasitical cotton aphid. The results showed that A. gossypii parasitized by B. communis, 2 glycolysis pathway genes, 5 fatty acid synthesis pathway genes, 6 glyceride synthesis pathway genes and 5 amino acid metabolism pathway key genes were all significantly up-regulated. Among them, the expression of glycerol kinase in the glyceride synthesis pathway decreased significantly, and there was no significant change in the expression of other genes. This study clarified the influence of B. communis on lipid synthesis of A. gossypii. The results are of great theoretical and practical value for in-depth understanding of the interaction between parasitic wasps and their hosts, and guiding artificial breeding of parasitoids and pest control.

Key words: Aphis gossypii, Binodoxys communis, lipid synthesis, fluorescence quantitative PCR, pest prevention and control

中图分类号:

S476.3

薛汇, 高雪珂, 常国凤, 崔金杰, 陈利珍. 广双瘤蚜茧蜂对棉蚜脂质合成通路的影响[J]. 中国生物防治学报, 2021, 37(6): 1204-1212.

XUE Hui, GAO Xueke, CHANG Guofeng, CUI Jinjie, CHEN Lizhen. Effects of Binodoxys communis Gahan on Aphis gossypii Glover Lipid Synthesis[J]. Chinese Journal of Biological Control, 2021, 37(6): 1204-1212.

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